[Objective] This study aimed to clone and analyze the sequence of CHS gene from Acer truncatum leaves. [Method] Using A. truncatum cultivars No.1-6 as experimental materials, total RNA was extracted from A. truncatum ...[Objective] This study aimed to clone and analyze the sequence of CHS gene from Acer truncatum leaves. [Method] Using A. truncatum cultivars No.1-6 as experimental materials, total RNA was extracted from A. truncatum leaves with the modified CTAB method. CHS gene sequences were downloaded from the NCBI and aligned by BLAST. Degenerate primers were designed by DNAMAN and Primer- premier5 to amplify the target band. CHS gene fragment was amplified by RT-PCR and ligated to pMD18-T vector. The identified positive colonies were sequenced. [Result] A 1 365 bp fragment was amplified. Sequence analysis suggested that the obtained fragment encoded 365 amino acids and shared above 90% homology to nucleotide sequence of CHS gene from A. palmatum and A. [Conclusion] In this study, CHS gene was successfully cloned from A. truncatum for the first time, which laid the foundation for efficient utilization of CHS gene.展开更多
Thirteen promising clones from population B3C2 potato genotypes （bred for quantitative resistance to late blight） obtained from the International Potato Center and three control cultivars were evaluated for four pla...Thirteen promising clones from population B3C2 potato genotypes （bred for quantitative resistance to late blight） obtained from the International Potato Center and three control cultivars were evaluated for four planting dates within two cropping seasons at Kalengyere Research Station in Southwestern Uganda in order to determine performance and yield stability. The analysis of variance of the relative area under disease progress curve （rAUDPC） revealed significant difference （P 〈 0.001） among genotype x planting date interaction, and significant difference （P 〈 0.001） among genotypes x fungicide treatments x planting date interaction. The analysis of variance （ANOVA） of yield revealed also significant difference among genotypes x planting date interaction and significant difference （P 〈 0.05） among genotypes x fungicide treatments x planting date interaction, showing the variable response of genotypes and the need for stability analysis. The additive main effects and multiplicative interactive （AMMI） statistical model showed that the most stable and high yielding genotypes were 396038.107, 396026.103 and 393280.82. The cultivars Victoria, Nakpot 5 and Cruza recorded low yields （below the average）, but Nakpot 5 was generally more variable, and is therefore highly adaptable to some environments.展开更多
基金Supported by Agricultural Improved Variety Project of Shandong Province(LKZ[2014]No.96)
文摘[Objective] This study aimed to clone and analyze the sequence of CHS gene from Acer truncatum leaves. [Method] Using A. truncatum cultivars No.1-6 as experimental materials, total RNA was extracted from A. truncatum leaves with the modified CTAB method. CHS gene sequences were downloaded from the NCBI and aligned by BLAST. Degenerate primers were designed by DNAMAN and Primer- premier5 to amplify the target band. CHS gene fragment was amplified by RT-PCR and ligated to pMD18-T vector. The identified positive colonies were sequenced. [Result] A 1 365 bp fragment was amplified. Sequence analysis suggested that the obtained fragment encoded 365 amino acids and shared above 90% homology to nucleotide sequence of CHS gene from A. palmatum and A. [Conclusion] In this study, CHS gene was successfully cloned from A. truncatum for the first time, which laid the foundation for efficient utilization of CHS gene.
文摘Thirteen promising clones from population B3C2 potato genotypes （bred for quantitative resistance to late blight） obtained from the International Potato Center and three control cultivars were evaluated for four planting dates within two cropping seasons at Kalengyere Research Station in Southwestern Uganda in order to determine performance and yield stability. The analysis of variance of the relative area under disease progress curve （rAUDPC） revealed significant difference （P 〈 0.001） among genotype x planting date interaction, and significant difference （P 〈 0.001） among genotypes x fungicide treatments x planting date interaction. The analysis of variance （ANOVA） of yield revealed also significant difference among genotypes x planting date interaction and significant difference （P 〈 0.05） among genotypes x fungicide treatments x planting date interaction, showing the variable response of genotypes and the need for stability analysis. The additive main effects and multiplicative interactive （AMMI） statistical model showed that the most stable and high yielding genotypes were 396038.107, 396026.103 and 393280.82. The cultivars Victoria, Nakpot 5 and Cruza recorded low yields （below the average）, but Nakpot 5 was generally more variable, and is therefore highly adaptable to some environments.
