本文以Lo and Mackinlay(1988,1989)[1][2]的方差比率为基础,构建了考察股市噪音成分的MCT指标,并对亚洲七个国家和地区的股市进行了检验。结果发现中国大陆和泰国股市具有更大的噪音成分,市场效率性较差;香港地区、台湾和日本股市则具...本文以Lo and Mackinlay(1988,1989)[1][2]的方差比率为基础,构建了考察股市噪音成分的MCT指标,并对亚洲七个国家和地区的股市进行了检验。结果发现中国大陆和泰国股市具有更大的噪音成分,市场效率性较差;香港地区、台湾和日本股市则具有较小的噪音交易成分,且无法拒绝不存在噪音交易的零假设;马来西亚和印度尼西亚的市场表现则处于前两者之间。最后我们通过Bootstrap对检验进行了重新评估,发现本文的检验结果是稳健的。展开更多
[Objective] This study aimed to establish a multiplex PCR system for de- tecting transgenic ingredients from Citrus. [Method] Based on the pBI121 plasmid sequences published in GenBank and actin gene sequence of Citru...[Objective] This study aimed to establish a multiplex PCR system for de- tecting transgenic ingredients from Citrus. [Method] Based on the pBI121 plasmid sequences published in GenBank and actin gene sequence of Citrus, the primers specific to CaMV35S promoter, NOS promoter, NOS terminator and actin gene were designed, to establish a multiple PCR system which could detect four types of sequences. In addition, orthogonal tests were performed to determine the optimal concentrations of all the components in PCR reaction system, as well as the optimal PCR cycle parameters. [Result] The optimal PCR reaction system should contain 2.5μl of 10xPCR buffer, 2.0μl of MgCI2 （25 mmol/L）, 2.0 μl of dNTP mixture （2.5 mmol/L of each dNTP）, 1.0 μl of actin gene primers （10μmol/L）, 1.0μl of 35S promoter primers （10 μmol/L）, 1.5 μl of NOS promoter primers （10 μmol/L） and 0.5 μl of NOS terminator primers （10μmol/L）, 0.1 μg of template DNA, 1.25 U of Taq DNA polymerase; ddH20 was added to the total reaction system of 25μl. The PCR reaction program consisted of pre-denaturing at 94℃ for 5 min; 31 cycles of denaturing at 94℃ for 30 s, annealing at 64.1℃ for 45 s and extension at 72℃ for 50 s; final extension at 72℃ for 10 min. The reaction system optimized with the orthogonal tests could detect as less as 0.1% transgenic component in the tested samples. [Conclusion] The MPCR detection system established in this study can meet the requirements in theory for detecting the genetically modified ingredients in Citrus or the deep-processed products.展开更多
[Objective] The aim was to compare the morphology difference between male and female individuals of Paramisgurnus dabryanus Sauvage. [Method] The comparison of 10 morphological characteristics between male and female ...[Objective] The aim was to compare the morphology difference between male and female individuals of Paramisgurnus dabryanus Sauvage. [Method] The comparison of 10 morphological characteristics between male and female individuals of P. dabryanus was conducted with principal component analysis and t-test methods. [Result] The results of t-test indicated that three of 10 morphological characteristics showed extremely significant difference（P0.01）and one of 10 morphological characteristics was significantly different（P0.05）. Principal component analysis revealed four principal components,the contribution rate of which was 31.151%,18.844%,12.324% and 10.169%,respectively. The cumulative contribution rate had reached 72.487%. The morphological variations between male and female individuals were not significant,which was consistent with that of t-test. [Conclusion] The result in this study had provided theoretical basis for the breeding of P. dabryanus.展开更多
文摘本文以Lo and Mackinlay(1988,1989)[1][2]的方差比率为基础,构建了考察股市噪音成分的MCT指标,并对亚洲七个国家和地区的股市进行了检验。结果发现中国大陆和泰国股市具有更大的噪音成分,市场效率性较差;香港地区、台湾和日本股市则具有较小的噪音交易成分,且无法拒绝不存在噪音交易的零假设;马来西亚和印度尼西亚的市场表现则处于前两者之间。最后我们通过Bootstrap对检验进行了重新评估,发现本文的检验结果是稳健的。
基金Supported by the Special Fund for Key Laboratories of Chongqing (CSTC)National Technology Research and Development Program of Ministry of Science and Technology for Countryside Field (863 Program,2011AA100205)+1 种基金Special Fund for Agro-scientific Research in the Public Interest of Ministry of Agriculture of China(201003067)Key Science and Technology Research Program of Ministry of Education of China (109131)~~
文摘[Objective] This study aimed to establish a multiplex PCR system for de- tecting transgenic ingredients from Citrus. [Method] Based on the pBI121 plasmid sequences published in GenBank and actin gene sequence of Citrus, the primers specific to CaMV35S promoter, NOS promoter, NOS terminator and actin gene were designed, to establish a multiple PCR system which could detect four types of sequences. In addition, orthogonal tests were performed to determine the optimal concentrations of all the components in PCR reaction system, as well as the optimal PCR cycle parameters. [Result] The optimal PCR reaction system should contain 2.5μl of 10xPCR buffer, 2.0μl of MgCI2 （25 mmol/L）, 2.0 μl of dNTP mixture （2.5 mmol/L of each dNTP）, 1.0 μl of actin gene primers （10μmol/L）, 1.0μl of 35S promoter primers （10 μmol/L）, 1.5 μl of NOS promoter primers （10 μmol/L） and 0.5 μl of NOS terminator primers （10μmol/L）, 0.1 μg of template DNA, 1.25 U of Taq DNA polymerase; ddH20 was added to the total reaction system of 25μl. The PCR reaction program consisted of pre-denaturing at 94℃ for 5 min; 31 cycles of denaturing at 94℃ for 30 s, annealing at 64.1℃ for 45 s and extension at 72℃ for 50 s; final extension at 72℃ for 10 min. The reaction system optimized with the orthogonal tests could detect as less as 0.1% transgenic component in the tested samples. [Conclusion] The MPCR detection system established in this study can meet the requirements in theory for detecting the genetically modified ingredients in Citrus or the deep-processed products.
基金Supported by Scientific and Technological Projects in Shandong Province~~
文摘[Objective] The aim was to compare the morphology difference between male and female individuals of Paramisgurnus dabryanus Sauvage. [Method] The comparison of 10 morphological characteristics between male and female individuals of P. dabryanus was conducted with principal component analysis and t-test methods. [Result] The results of t-test indicated that three of 10 morphological characteristics showed extremely significant difference（P0.01）and one of 10 morphological characteristics was significantly different（P0.05）. Principal component analysis revealed four principal components,the contribution rate of which was 31.151%,18.844%,12.324% and 10.169%,respectively. The cumulative contribution rate had reached 72.487%. The morphological variations between male and female individuals were not significant,which was consistent with that of t-test. [Conclusion] The result in this study had provided theoretical basis for the breeding of P. dabryanus.
