不同离心方式对成分血分离的影响

目的:探讨不同离心方式对成分血分离的影响。方法:选取2010年4月-2011年3月我站制备的4272袋全血,采用不同的离心方式和血袋摆放方式,观察两种方式对成分血离心效果的影响和血袋破损率。结果:观察组采用横袋摆放,对照组采用竖袋摆放,采用不同的离心力和离心时间,观察组离心效果和破损率明显优于对照组。结论:改进离心方式和血袋方式可有效提高成分血分离效率和减少血袋破损率。

全血成分分离机最佳参数的设定和影响因素分析

目的应用全血成分分离机采用白膜法制备浓缩血小板,寻找分离机的最佳参数。方法选择不同的运行参数制备血小板,对血小板及红细胞回收率进行比较,分析重新离心和轻微脂肪血对回收率的影响。结果确定参数posdet1(光电探头第1次探测的位置,决定富含血小板血浆层的转移量)为4时,血小板回收率最高约为70%,重新离心对血小板回收率无影响,轻微脂肪血的血小板回收率偏低。结论posdet1为4是白膜法机器分离血小板的最佳参数。

全自动全血成分分离机制备洗涤红细胞效果观察

目的观察全自动全血成分分离机制备洗涤红细胞的效果是否符合国家质量要求。方法设置全自动全血成分分离机的运行参数,制备30袋洗涤红细胞,检测洗涤红细胞的容量、血红蛋白含量、上清蛋白质含量和溶血率。结果 30袋洗涤红细胞的检测指标均符合国标质量要求,容量(m L):248.367±3.685,血红蛋白含量(g):43.727±1.681,上清蛋白质含量(g):0.014±0.005,溶血率(%):0.037±0.008。结论全自动全血成分分离机制备的洗涤红细胞符合国家质量要求。

全自动全血成分分离机白膜法在制备浓缩血小板中的应用效果

目的探讨全自动全血成分分离机白膜法在制备浓缩血小板中的应用效果。方法选取2020年1月至2020年12月茂名市中心血站收集的108袋400 ml全血作为研究对象,采用随机数字表法分为对照组(54袋)与实验组(54袋)。对照组在22℃的条件下离心,采用人工白膜法制备浓缩血小板;实验组在22℃的条件下离心,使用全自动全血成分分离机白膜法制备浓缩血小板。比较两组的浓缩血小板合格率、浓缩血小板计数、酸碱度。结果实验组的浓缩血小板计数大于对照组,差异有统计学意义(P<0.05);两组的酸碱度比较,差异无统计学意义(P>0.05);实验组红细胞计数小于对照组,差异有统计学意义(P<0.05);两组的浓缩血小板合格率比较,差异无统计学意义(P>0.05)。结论采用全自动全血成分分离机制备的浓缩血小板的效果优于人工方法。

全血成分分离机实现标识条码校验分离血液效率的分析

分析在制备程序中实现标识条码定位和条码比对法分析分离血液效率影响。方法:在制备程序中增加全血袋、转移袋的唯一定位标识条码,然后进行联袋条码比对,最后与血站采供血系统进行数据联网对接。结果:通过标识条码定位,使操作者操作更加规范、标准;通过联袋条码比对,保证血袋条码一致性;通过信息化接口,实现加工制备后的血液产品数据一次性批量转换,不需要在采供血系统进行逐袋入库扫描。结论:即实现了多联袋条码比对,又保证了血液产品质量,又缩短了加工制备时间,间接提高了工作效率。

EFFECT OF HERB-MEDICINE-CAKE-SEPARATED MOXIBUSTION ON SERUM LIPOPROTEIN CONTENTS AND RATIO OF HDL-Ch AND LDL-Ch IN HYPERLIPEMIA RABBITS

Objective: To observe the effect of herb-medicine-cake-separated moxibustion on serum lipoprotein in hyperlipemia rabbits. Methods: 55 New-Zealand rabbits were randomly divided into control group (n=13), model group (n=14), direct moxibustion group (n=14) and herb-medicine-cake-separated moxibustion (indirect moxibustion) group (n=14). Hyperlipemia model was established by feeding the animals with specialized forage (15% vitellus powder, 5% lard, 0.5% cholesterol and common forage) for 6 weeks. Moxibustion was applied to “Juque”(CV 14), “Tianshu”(ST 25), “Fenglong”(ST 40), etc., 4 moxa-cones for every acupoint, once daily and continuously for 40 days. Serum high density lipoprotein-cholesterol (HDL-Ch), low density lipoprotein-cholesterol (LDL-Ch) and total cholesterol (TCh) contents were assayed with colorimetric method. Results: Compared with control group, serum LDL-Ch content, HDL-Ch/LDL-Ch and HDL-Ch/TCh of model group were significantly higher (P<0.05～0.01), while compared with model group, LDL-Ch contents of two moxibustion groups were strikingly lower (P<0.01). No significant differences were found between two moxibustion groups in all the 4 indexes. Conclusion: Both direct and indirect moxibustion can effectively lower serum LDL-Ch, raise HDL-Ch, HDL-Ch/LDL-Ch and HDL-Ch/TCh, and regulate lipoprotein metabolism in hyperlipemia rabbits.

EFFECT OF HERB-MEDICINE-CAKE-SEPARATED MOXIBUSTION ON SERUM LIPOPROTEIN CONTENTS IN HYPERLIPEMIA RABBITS

Objective: To study the effect of medicinal herb-cake-separated moxibustion on serum lipoprotein levels in hyperlipemia rabbits. Methods: A total of 55 New-Zealand rabbits were randomly divided into control group (n=13), model group (n=14), direct moxibustion group (n=14) and medicinal herb-cake-separated moxibustion (indirect moxibustion) group (n=14). Hyperlipemia model was established by feeding the animals with specialized forage (15% vitellus powder, 5% lard, 0.5% cholesterol and common forage) for 6 weeks. Moxibustion was applied to “Juque”(CV 14), “Tianshu”(ST 25), “Fenglong”(ST 40), etc., 4 moxa-cones for every acupoint, once daily and continuously for 40 days. Serum triglyceride (TG) and total cholesterol (TCh) contents were assayed with colorimentric method. Results: Compared with control group, TCh and TG levels of model group increased significantly (P< 0.01). TCh and TG contents of direct moxibustion and indirect moxibustion groups were significantly lower than those of model group (P<0.01). Comparison between two moxibustion groups showed that serum TCh level of indirect moxibustion group was strikingly lower than that of direct moxibustion group (P<0.01). It indicated that both direct and indirect moxibustion could effectively lower hyperlipemia and the therapeutic effect of indirect moxibustion was significantly superior to that of direct moxibustion in lowering serum TCh level. Conclusion: Both direct and indirect moxibustion can regulate lipid metabolism and the therapeutic effect of medicinal herb-cake-separated moxibustion is superior to that of direction moxibustion in hyperlipemia rabbits.