应用古地貌成因组合识别法恢复塔河油田主体区古岩溶地貌

针对古地貌控制了岩溶储集空间的形成与展布,探索应用古地貌成因组合法恢复塔河油田主体区前石炭纪古岩溶地貌。在岩溶台地、岩溶缓坡地、岩溶陡坡地、岩溶山间盆地等4类二级地貌单元的基础上,增加地貌识别因子,进一步划分出10种三级地貌单元,提高了古地貌形态识别的精度。结合微地貌单元岩溶发育特征和油气储集性能,分析油气产量与地貌的关系,为岩溶储集层预测提供依据。

下二门油田上层系三角洲前缘砂体成因组合和分布规律

下二门油田上层系为一套辨状河三角洲前缘沉积砂体组合。根据岩心资料、录井资料和测井资料共识别出了水下分流河道、河口坝、席状砂、远砂坝、前缘滑塌沉积5种成因砂体和叠置的水下分流河道砂体、叠置的河口坝砂体、水下分流河道-河口坝组合砂体、席状砂-河口坝组合砂体、席状砂-水下分流河道组合砂体、席状砂-滑塌重力流组合砂体等六种砂体组合。在此基础上。从盆底物源供给特征、湖平面变化和湖底地形特点,提出了进积式、退积式和局部低洼堆积式三种三角洲前缘沉积模式.分析了各模式砂体组合在时空上的分布特征.认为本区发育进积式和局部低洼堆积式两种沉积模式。

三角洲前缘储集砂体的成因组合与分布规律——以松辽盆地大老爷府地区白垩系泉头组四段为例

以松辽盆地大老爷府油田白垩系泉头组四段为例,运用高分辨率层序地层学短期基准面旋回原理,详细刻画了以水下分流河道和河口坝沉积为主导的三角洲前缘储集砂体的成因组合特征,提出将其划分为水下分流河道成因组合、水下分流河道与河口坝成因组合和河口坝成因组合三种类型,并对这些储集砂体成因组合进行了详细解剖,例如,将水下分流河道成因组合划分为完整型、废弃型和多期叠加型水下分流河道组合三种亚类型;河口坝成因组合划分为完整型和叠加型河口坝成因组合两种亚类型;将复合成因砂体描述为坝上河成因组合。研究表明各种成因组合的发育受沉积基准面升降,可容纳空间增加与沉积物补给通量比值变化的控制。在此基础上,文章讨论了各种成因组合砂体在高分辨率层序地层学格架内的时空分布规律。

Generating test cases for software configuration testing

Software configuration testing is used to test a piece of software with all kinds of hardware to ensure that it can run properly on them. This paper generates test cases for configuration testing with several common methods, such as multiple single-factor experiments, uniform design, and orthogonal experiment design used in other fields. This paper analyzes their merits and improves the orthogonal experiment design method with pairwise testing, and decreases the testing risk caused by incomplete testing with a method of multiple-factors-covering. It presents a simple factor cover method which can cover all the factors and pairwise combinations to the greatest degree. Some comparisons of these methods are made on the aspects of test suite scale, coverage, and usability, etc..

东南沿海中生代侵入岩及与火山岩的时空关系

东南沿海中生代侵入岩可分为三套,与火山活动有关的第二套,第三套侵入岩在浙东——闽东区有成分上对应的火山岩。下火山岩系和第二套侵入岩是挤压条件下地壳分熔的英安质岩浆经分离结晶形成;上火山岩系和第三套侵入岩是扩张条件上地幔和下地壳分熔形成的双峰式系列,形成高碱流纹岩和碱性花岗岩的A型岩浆地幔物质所占比例最高,由北而南,火山岩覆盖面积渐小,粤东区第二套侵入岩与下火山岩系相比,缺中性岩类,下地壳分熔形成同熔型二长花岗岩及其火山岩,黑云母花岗岩是熔融区上升迁移的结果,第三套侵入岩出露局限。

开合构造与岩浆活动

研究了开合构造与岩浆活动的关系。对与开合构造有关的火成岩的分类、命名、系列、组合、类型、成因进行了分析;对构造-岩浆活动的旋回进行了讨论,应用岩石地球化学图解法阐述了构造环境的演化,估算了开合作用的速度与距离;简介了中国一些地区开合构造-岩浆演化的实例。

Correlation Analysis Between Yield Components and Yield Per Plant of Double-low Hybrid Rapeseed

[Objective] The experiment aimed to study the relation between yield components and yield per plant of double-low hybrid rapeseed and provide reference for breaking yield limitation of rapeseed and culturing new double-low hybrid rapeseed variety. [Method]The yield components and yield per plant of two cross combination of double-low hybrid rapeseed (B02, D04) and Shuza 6 were correlatively analyzed and compared, besides, the path analysis was also carried on to them. [Result] Among B02, D04 and Shuza No.6, effective pod number per plant and seeds per silique, seeds per pod and 1 000-grain weight were all negative correlation. In high yield hybrid, pod number per plant, seeds per pod had more impaction on yield per plant than 1 000-grain weight and the difference was at 0.01 significant level. In the control variety Shuza No.6, the impactions of pod number per plant and seeds per pod on yield per plant were bigger than that of 1 000-grain weight on yield per plant, however, the difference was not significant. [Conclusion] The improvement of effective pod number per plant was an important aim of breeding work of double low rapeseed breeding in Sichuan ecological region.

Comparison of Newly Synthetic Hexaploid Wheat with Its Donors on SSR Products

Microsatellites or SSRs as powerful genetic markers have widely been used in genetics and evolutionary biology in common wheat. Because of the high polymorphism, newly synthesized hexaploid wheat has been used in the construction of genetic segregation population for SSR markers, However, data on the evolution of microsatellites during the polyploidization event of hexaploid wheat are limited. In this study, 66 pairs of specific to A/B genome SSR patterns among newly synthesized hexaploid wheat, the donor tetraploid wheat and Aegilops tauschii were compared. The results indicated that most SSR markers were conserved during the polyploidization events of newly synthetic hexaploid wheat, from Triticum turgidum and Ae. tauschii. Over 70% A/B genome specific SSR markers could amplify the SSR sequences from the D genome ofAe. tauschii. Most amplified fragments from Ae, tauschii were detected in synthetic hexaploid at corresponding positions with the same sizes and patterns as in its parental Ae. tauschii. This suggested that these SSR markers, specific for A/B genome in common wheat, could amplify SSR products of D genome besides A/B genome in the newly synthesized hexaploid wheat, that is, these SSR primers specific for A/B genome in common wheat were nonspecific for the A/B genome in the synthetic hexaploid wheat. In addition, one amplified Ae. tauschii product was not detected in the newly synthetic hexaploid wheat. An extra-amplified product was found in the newly synthetic hexaploid wheat. These results suggested that caution should be taken when using SSR marker to genotype newly synthetic hexaploid wheat.

安塞浅水三角洲前缘复合单砂体精细识别与划分

鄂尔多斯盆地东缘安塞浅水三角洲前缘长6储层单期河道砂体中心迁移频率较快,导致储层物性差异较大,由于对砂体展布规律认识不足,普遍存在注采不对应、宏观剩余油分布具强分散等问题。在野外露头的指导下,依据测井数据,以三大类单一成因砂体的7种12类接触组合关系,对研究区复合单砂体进行表征:水下分流河道砂体作为骨架砂体发育最广,强分流改道与汇流区域普遍发育"水下分流河道-水下分流河道"型;席状砂沟通其他类型砂体时油层连片分布,但侧向切叠的席状砂不具备挖潜条件;分离型砂体在下游区最为发育,砂体直接接触泥岩,却不为井网所控制。该项研究为油田开发中后期剩余油的挖潜提供可操作的地质依据。

Total Chemical Synthesis, Assembly of Human Torque Teno Virus Genome

Torque teno virus(TTV) is a nonenveloped virus containing a single-stranded,circular DNA genome of approximately 3.8kb.We completely synthesized the 3 808 nucleotides of the TTV(SANBAN isolate) genome,which contains a hairpin structure and a GC-rich region.More than 100 overlapping oligonucleotides were chemically synthesized and assembled by polymerase chain assembly reaction(PCA),and the synthesis was completed with splicing by overlap extension(SOEing).This study establishes the methodological basis of the chemical synthesis of a viral genome for use as a live attenuated vaccine or gene therapy vector.

Pore constitution and tortuosity factor of reactively synthesized porous TiAl intermetallic compound

The pore constitution and tortuosity factor of porous TiAl intermetallic were studied on the basis of the variation behavior of pore structure parameters and the discrete particle model. The pore formation mechanism of porous TiAl is mainly ascribed to three aspects: the clearance space in green compact, the diffusive pores in the reaction process and the phase transition pores, resulting in the open porosities of 5.6%, 42.9% and 1.3%, respectively. According to the Hagen-Poiseuille equation, the tortuosity factor of porous TiAl is determined in the range of 1.3-2.2. Based on the discrete particle model and the variation rule of the tortuosity factor, the tortuosity factor depends mainly on the parameters of fabrication constant, particle shape factor, clearance distance and powder particle size. The quantitative relationships among them have been established, which can be used as the basis for adjusting the pore structure of porous intermetallics.

Recent advances in the molecular genetics of resin biosynthesis and genetic engineering strategies to improve defenses in conifers

Since the first terpenoid synthase cDNA was obtained by the reverse genetic approach from grand fir, great progress in the molecular genetics of terpenoid formation has been made with angiosperms and genes encoding a monoterpene synthase, a sesquiterpene synthase, and a diterpene synthase. Tree killing bark beetles and their vectored fungal pathogens are the most destructive agents of conifer forests worldwide. Conifers defend against attack by the constitutive and inducible production of oleoresin that accumulates at the wound site to kill invaders and both flush and seal the injury. Although toxic to the bark beetle and fungal pathogen, oleoresin also plays a central role in the chemical ecology of these boring insects. Recent advances in the molecular genetics of terpenoid biosynthesis provide evidence for the evolutionary origins of oleoresin and permit consideration of genetic engineering strategies to improve conifer defenses as a component of modern forest biotechnology. This review described enzymes of resin biosynthesis, structural feathers of genes genomic intron and exon organization, pathway organization and evolution, resin production and accumulation, interactions between conifer and bark beetle, and engineering strategies to improve conifer defenses.

Relationship between Mutations in DNA Sequences Loci Coding Pre-miRNAs and Genes Related to Biogenesis of SncRNAs with MiRNA Expression in Endometrial Carcinoma Tissues

Endometrial cancer （EC） is the most common and lethal gynaecological cancer type in Europe and in North America. Frequently EC arises more in the corpus proper and manifests as round, polypoid expansile masses, but it may also originate in the lower uterine segment or spread in endometrium with necrosis and hemorrhage. The analysis was performed using a custom panel containing all DNA sequences loci coding pre-miRNAs and genes related to biogenesis and regulation of sncRNAs in normal and tumor tissues extracted from 6 unrelated patients with endometrial carcinoma. The identified variations were correlated with mature miRNAs differentially expressed in the same normal and tumor endometrial tissues. The comparison analysis confirmed the high degree of cellular and genetic intratumoral heterogeneity with a temporal and spatial miRNA expression distribution in association with genomic variants identified. The classification of specific DNA mutations, onto the loci identified, should be suitable to characterize possible instability genome regions and help classification of tumors to ameliorate the clinical management of patients affected by endometrial carcinoma.

Combinatorial effects of NaomaiYihao Capsules(脑脉一号胶囊) and vascular endothelial growth factor gene-transfected bone marrow mesenchymal stem cells on angiogenesis in cerebral ischemic tis sues in rats

OBJECTIVE:To investigate the combinatorial effects of Naomai Yihao(NMYH) Capsules(脑脉一号胶囊) and vascular endothelial growth factor(VEGF) gene-transfected bone marrow mesenchymal stem cells(BMSCs) on angiogenesis in cerebral ischemic tissues in rats and the mechanism.METHOD:BMSCs were isolated and cultured from bone marrow by an adherence method.Then,BMSCs were transfected with the eukaryotic expression plasmid pEGFP-VEGF 165 by positive ionic liposome transfection.A rat model of middle cerebral artery occlusion(MCAO) was established.Rats were allocated to six groups:model,BMSC,VEGF gene-transfected BMSC transplantation(BMSC/VEGF),NMYH,combined NMYH and BMSC/VEGF(combined treatment group) and sham operation groups.The behavioral rating score(BRS) of rat and the expression of CD34 and VEGF in brain tis sue were measured by immunohistochemistry on days 7,14 and 21 after reperfusion.Angiogenesi was observed and evaluated with laser scanning confocal microscopy.RESULTS:The BRS of rats in NMYH,BMSC transplan tation and combined treatment groups was significantly lower than that of the model group(P< 0.001),with no significant difference between NMYH and transplantation groups(P=0.619).The expression of CD34 andVEGF in NMYH,transplanta tion and combined treatment groups increased(P< 0.001),with a significant difference between NMYH and transplantation groups(P<0.001).The blood vessel area in NMYH,transplantation and com bined treatment groups was significantly increased(P<0.05),without a significant difference between NMYH and transplantation groups(P=0.873).CONCLUSIONS:VEGF gene-transfected BMSCs im prove angiogenesis in the cerebral ischemic area NMYH Capsules promote angiogenesis in MCAO rats treated with BMSC transplantation,which show an improved BRS.The mechanism of angio genesis may be related to up-regulation ofVEGF ex pression.

Conversion of the high-yield salinomycin producer Streptomyces albus BK3-25 into a surrogate host for polyketide production

An ideal surrogate host for heterologous production of various natural products is expected to have efficient nutrient utilization,fast growth,abundant precursors and energy supply,and a pronounced gene expression.Streptomyces albus BK3-25 is a high-yield industrial strain producing type-Ⅰ polyketide sahnomycin,with a unique ability of bean oil utilization.Its potential of being a surrogate host for heterologous production of PKS was engineered and evaluated herein.Firstly,introduction of a three-gene cassette for the biosynthesis of ethylmalonyl-CoA resulted in accumulation of ethylmalonyl-CoA precursor and sahnomycin,and subsequent deletion of the sahnomycin biosynthetic gene cluster resulted in a host with rich supplies of common polyketide precursors,including malonyl-CoA,methylmalonyl-CoA,and ethylmalonyl-CoA.Secondly,the energy and reducing force were measured,and the improved accumulation of ATP and NADPH was observed in the mutant.Furthermore,the strength of a series of selected endogenous promoters based on microarray data was assessed at different growth phases,and a strong constitutive promoter was identified,providing a useful tool for further engineered gene expression.Finally,the potential of the BK3-25 derived host ZXJ-6 was evaluated with the introduction of the actinorhodin biosynthetic gene cluster from Streptomyces coelicolor,and the heterologous production of actinorhodin was obtained.This work clearly indicated the potential of the high-yield sahnomycin producer as a surrogate host for heterologous production of polyketides,although more genetic manipulation should be conducted to streamline its performance.

Development and characterization of synthetic amphiploids of Oryza sativa and Oryza latifolia

Oryza sativa and Oryza latifolia belong to the AA and CCDD genomes of Oryza, respectively. In this study, amphiploids were obtained from the tube seedlings of O. sativa × O. latifolia F1 hybrids by treatment with colchicine, an agent for chromosome doubling. Subse- quently, amphiploids were investigated using the methods of morphology, genomic in situ hybridization, and molec- ular markers. Amphiploids were characterized by a shorter plant height, larger diameter of stem, longer and wider leaves, darker leaf color, decreased spikelets per panicle and panicle length, and larger spikelets and anthers than the original F1 hybrid. Based on the mitotic metaphase chro- mosome number of the investigated root tips, the somatic chromosome number of the amphiploid is 2n = 72. Additionally, the amphiploid is an allohexaploid, and its genomic constitution is AACCDD by genomic in situ hybridization analysis. Finally, the amphiploids were identified to be true using 37 polymorphic markers at the DNA level.

Amplified circularly polarized luminescence promoted by hierarchical selfassembly involving Pt···Pt interactions

The design and construction of highly effective circularly polarized luminescence(CPL)-active materials has aroused considerable attention due to their widespread applications in sensors,optical devices,and asymmetric synthesis.However,the exploration of novel CPL-active materials with high luminescence dissymmetry factor(glum)values is still a challenge.Herein,we describe a new approach for the preparation of supramolecular metallacycles with amplified CPL promoted by hierarchical self-assembly involving Pt···Pt interactions.Notably,the resultant metallacycles exhibited strong CPL signals with high glum values,while their corresponding precursors were CPL silent.The CPL amplification mechanism was comprehensively validated by ultravioletvisible absorption,emission spectroscopy,nuclear magnetic resonance spectroscopy,scanning electron microscopy,transmission electron microscopy,atomic force microscopy,coarse-grained molecular dynamics simulations,and timedependent density functional theory calculations.This work thus provides the first example of preparing highly effective CPL-active materials based on hierarchical self-assembly involving Pt···Pt interactions.