The objective of this research was to study the effect of ECS on cumulus expansion and rate of nucleus maturation of bovine oocytes. Media maturation were used: (1) TCM 199 + FCS 10%; (2) TCM 199 + FCS 10% + E...The objective of this research was to study the effect of ECS on cumulus expansion and rate of nucleus maturation of bovine oocytes. Media maturation were used: (1) TCM 199 + FCS 10%; (2) TCM 199 + FCS 10% + ECS 5%; (3) TCM 199 + FCS 10% + ECS 7, 5%; (4) TCM 199 + FCS 10% + ECS 10%; (5) TCM 199 + ECS 10%. Supplementation of ECS had significantly difference (P 〈 0.05) on expansion of cumulus cells and rate of nucleus maturation. Supplementation of ECS 5% was the best result in expanded cumulus cells and metaphase II rate: 82% and 72% respectively. It was concluded that medium of TCM 199 + FCS 10%o + ECS 5% was the best maturation medium展开更多
文摘The objective of this research was to study the effect of ECS on cumulus expansion and rate of nucleus maturation of bovine oocytes. Media maturation were used: (1) TCM 199 + FCS 10%; (2) TCM 199 + FCS 10% + ECS 5%; (3) TCM 199 + FCS 10% + ECS 7, 5%; (4) TCM 199 + FCS 10% + ECS 10%; (5) TCM 199 + ECS 10%. Supplementation of ECS had significantly difference (P 〈 0.05) on expansion of cumulus cells and rate of nucleus maturation. Supplementation of ECS 5% was the best result in expanded cumulus cells and metaphase II rate: 82% and 72% respectively. It was concluded that medium of TCM 199 + FCS 10%o + ECS 5% was the best maturation medium