[Objective]This study was to screen out suitable genotypes and basic medium for the culture of maize mature embryos.[Method]Using mature embryos of nine maize genotypes as explants,the effects of genotypes and basic m...[Objective]This study was to screen out suitable genotypes and basic medium for the culture of maize mature embryos.[Method]Using mature embryos of nine maize genotypes as explants,the effects of genotypes and basic medium on callus induction and subculture were investigated.[Result]The genotypes performed better in callus induction and subculture were found in turn 853-35,853-209,Dan 34 and 81162.MS medium is better than N6 medium in the callus induction from maize embryos,while N6 medium is more suitable for callus subculture.[Conclusion]Our study further improved the tissue culture system in maize with mature embryos as explants.展开更多
Long juvenile phase and lack of effective protocols for large scale vegetative propagation are limitations to domestication and improvement of the shea tree. The present study seeks to develop a protocol for plant reg...Long juvenile phase and lack of effective protocols for large scale vegetative propagation are limitations to domestication and improvement of the shea tree. The present study seeks to develop a protocol for plant regeneration of shea （Vitellaria paradoxa） from immature cotyledon explants. Embryogenic callus cultures were induced on Murashige and Skoog medium （MS） containing 3% sucrose, 0.24% Phytagel, and various concentrations of 2,4-Dichlorophenoxyacetic acid （2,4-D） after four weeks of culture in darkness. Rates of embryogenic callus induction were significantly affected by the addition of 2, 4-D to the medium. Within 28 days of culture, the highest percentage of embyogenic calli （77.61%） occurred on MS media containing 0.45 ~tM of 2,4-D in the dark. Somatic embryos were obtained by culturing embryogenic callus （in the dark） on MS medium fortified with 3% sucrose, 0.24% phytagel and devoid of growth regulators. Culturing at 16 h photoperiod restricted both the induction of embryogenic calli cultures and somatic embryos. Somatic embryos germinated, developed shoots and rooted vigorously on MS medium devoid of growth regulators. Germinated plantlets were acclimatized, successfully.展开更多
A micropropagation technique is developed for the multiplication of Dendrocalamus strictus （D. strictus）, Dendrocalamus asper （19. asper） and Bambusa bambos （B. bambos） through shoot proliferation. Nodal explant...A micropropagation technique is developed for the multiplication of Dendrocalamus strictus （D. strictus）, Dendrocalamus asper （19. asper） and Bambusa bambos （B. bambos） through shoot proliferation. Nodal explants obtained from field gown clumps were used to initiate cultures. Shoots were induced on Murashige and Skoog＇s （MS） medium supplemented with 5 mg L^-1 6-benzylamino purine （BAP）. Rapid shoot multiplication was obtained on MS medium containing 3 mg Lt BAP in D. asper, B. bambos and 2 mg L^-1 BAP in D. strictus. In vitro multiplied shoots showed best root induction on half strength MS supplemented with 1 mg L^-1 indole-3 butyric acid （IBA） and 0.5 mg L^-1 1-naphthalene acetic acid （NAA） in D. asper. Pre-rooting conditioning followed by culturing on half strength MS supplemented with 1 mg L^-1 IBA and 2 mg L^-1 IBA showed maximum root induction in D. strictus and B. bambos, respectively. Further root proliferation was obtained on hormone free medium. The micropropagated plantlets were acclimatized and successfully transferred on soil in green house.展开更多
基金Supported by National Natural Science Foundation of China(31070224)National Natural Science Foundation of China(30970219)Key Project from Science and Technology Department in Jilin Province(20080203)~~
文摘[Objective]This study was to screen out suitable genotypes and basic medium for the culture of maize mature embryos.[Method]Using mature embryos of nine maize genotypes as explants,the effects of genotypes and basic medium on callus induction and subculture were investigated.[Result]The genotypes performed better in callus induction and subculture were found in turn 853-35,853-209,Dan 34 and 81162.MS medium is better than N6 medium in the callus induction from maize embryos,while N6 medium is more suitable for callus subculture.[Conclusion]Our study further improved the tissue culture system in maize with mature embryos as explants.
文摘Long juvenile phase and lack of effective protocols for large scale vegetative propagation are limitations to domestication and improvement of the shea tree. The present study seeks to develop a protocol for plant regeneration of shea （Vitellaria paradoxa） from immature cotyledon explants. Embryogenic callus cultures were induced on Murashige and Skoog medium （MS） containing 3% sucrose, 0.24% Phytagel, and various concentrations of 2,4-Dichlorophenoxyacetic acid （2,4-D） after four weeks of culture in darkness. Rates of embryogenic callus induction were significantly affected by the addition of 2, 4-D to the medium. Within 28 days of culture, the highest percentage of embyogenic calli （77.61%） occurred on MS media containing 0.45 ~tM of 2,4-D in the dark. Somatic embryos were obtained by culturing embryogenic callus （in the dark） on MS medium fortified with 3% sucrose, 0.24% phytagel and devoid of growth regulators. Culturing at 16 h photoperiod restricted both the induction of embryogenic calli cultures and somatic embryos. Somatic embryos germinated, developed shoots and rooted vigorously on MS medium devoid of growth regulators. Germinated plantlets were acclimatized, successfully.
文摘A micropropagation technique is developed for the multiplication of Dendrocalamus strictus （D. strictus）, Dendrocalamus asper （19. asper） and Bambusa bambos （B. bambos） through shoot proliferation. Nodal explants obtained from field gown clumps were used to initiate cultures. Shoots were induced on Murashige and Skoog＇s （MS） medium supplemented with 5 mg L^-1 6-benzylamino purine （BAP）. Rapid shoot multiplication was obtained on MS medium containing 3 mg Lt BAP in D. asper, B. bambos and 2 mg L^-1 BAP in D. strictus. In vitro multiplied shoots showed best root induction on half strength MS supplemented with 1 mg L^-1 indole-3 butyric acid （IBA） and 0.5 mg L^-1 1-naphthalene acetic acid （NAA） in D. asper. Pre-rooting conditioning followed by culturing on half strength MS supplemented with 1 mg L^-1 IBA and 2 mg L^-1 IBA showed maximum root induction in D. strictus and B. bambos, respectively. Further root proliferation was obtained on hormone free medium. The micropropagated plantlets were acclimatized and successfully transferred on soil in green house.
