新型超滤系统处理的成牛血清在细胞培养的应用及其对产毒效果的影响

为得到符合或优于牛血清质量标准的成牛血清,利用新型超滤系统对细胞培养用成牛血清原料进行处理。经过对3批已处理的成牛血清进行无菌检验、细胞促生长试验及接毒后产毒效果等各项检验,结果表明,经该超滤纯化工艺处理的成牛血清可替代新生牛血清用于细胞培养,且对产毒效果无影响。

胎牛及成牛皮胶原性质的研究

采用低温酶解法分别从胎牛及成牛二层皮中提取了可溶性胶原,对其进行了表面形貌、含水量、等电点、热稳定性的测定,并采用红外光谱对其结构特性进行了表征。结果表明:胎牛皮胶原的多孔网状结构明显,内部含水量高于成牛,但热稳定性低于成牛。红外光谱结果表明:与胎牛皮胶原相比,成牛皮胶原中含有更多成熟稳定的交联结构。因此,说明随着动物的年龄增长,由于胶原类型及交联含量的改变,胶原的可溶性逐渐降低。

成牛血清低分子量成分在杂交瘤细胞体外“无血清”培养中的利用

利用适量的(3.5—14%)成牛血清低分子成分超滤液(LMW-CS,排阻M=300 00)、10mg/L转铁蛋白(T)、10 mg/L胰岛素(Ⅰ)、20μ mol/L乙醇胺(E)、40 n mol/L硒酸钠(S,当以RPMT1640为基础液时用)等诸补充成分替代胎牛血清加到基础液RPMI1640或1:1(v/v)的IMDM与Ham's F_(12)混合液中构成本实验性'无血清'培养基。培养在此种培养基中的杂交瘤细胞其最高细胞密度可以达到甚至超过培养在含胎牛血清的生长培养基中的最高细胞密度。对于杂交癌细胞生长,4%的LMW-CS基本满足要求,T、I、E、S四种补充成分中乙醇胺(E)是首位影响因子;LMW-CS与TIES两部分之间的协同作用极为显著,两者缺一不可。

成牛逆呕食团治疗犊牛下痢

下痢是威胁哺乳犊牛生长发育的主要疾病之一,若病程稍拖长或时而好转时而拉痢,都将影响犊牛的生长发育,对其体型、机能、健康及生产性能都会产生不同程度的影响,甚至导致死亡,造成不必要的经济损失.近几年来,笔者采用成年健康牛逆呕出来的食团喂服下痢哺乳犊牛,效果较理想,现叙述如下.

Composition Analysis on Intermuscular Fatty Acid of Qinghai Datong Yak

[ Objective] The study aimed to provide a theoretical basis for rational utilization of Datong yak resource. [ Method] By qualitative and quantitative determination with gas chromatograph, the composition and content of intermuscular fatty acid of Datong yak were compared with those of Datong yellow cattle. [ Result] Comparing with Datong yellow cattle, Datong yak had lower saturated fatty acid （SFA） content and higher polyunsaturated fatty acid （PUFA） content at extremely significant level （P 〈0.01 ）, while the difference of monounsaturated fatty acid （MUFA） contents between them was not significant （P〉0.05）. The PUFA/SFA and n-6/n-3 PUFA of Datong yak were in accordance with the recommended proportions. [ Conclusion] The intermuscular fatty acid composition of Datong yak is superior to that of local yellow cattle, which indicates that Datong yak meat has high nutritive value and wide development prospect.

肉牛育肥饲料配方(供参考)

现在牛的价格暂时走向低谷,人们一时卖不出好价钱就灰心丧气,有的甚至越低越卖,而我认为价格越低越要养,越要养好,肉牛育肥牛膘肥,肉又多又嫩,肯定会卖出个好价钱,现在是好牛好价,次牛次价,为此,特向养殖场、户介绍几种育肥牛的饲料配方,供参考.

Optimizing Yanbian Cow Oocytes Mature in vitro and Cleavage System after Nuclear Transfer Based on Uniform Design

[Objective] The aim was to optimize Yanbian cow oocytes mature in vitro and cleavage system after nuclear transfer based on uniform design. [Method] Oocytes were recovered by aspiration method, and oocytes were matured in vitro （IVM） with different conditions, and then carried out nucleus transfer, fusion, activation and in vitro culture （IVC） of embryo. Effects of ovary storage temperature, maturation time and follicular diameter size on in vitro maturation and cleavage rates of cow oocytes were compared. [ Result] The best conditions of IVM of Yanbian cow oocytes was that： the oocytes of 8 mm diameter were matured in vitro for 24 hours when the ovaries were stored at 26℃ or 31 ℃. The best cleave conditions after nucleus transfer of oocytes was that： the oocytes of 6 mm or 8 mm diameter were cultured in vitro for 24 hours when the ovaries were stored at 26℃. [ Conclusion] The result has some reference to Yanbian cow and other cow breeding and population expanding propagation.

Improvement Effect of Beef Cattle Frozen Semen of Different Varieties for Local Yellow Cattle

In the improvement process of local yellow cattle with frozen semen of beef cattle, the cows were grouped according to different cross combinations. Then, the conception rate, dystocia rate and reproductive survival rate of the cows in each group, as well as the body size and body weight of new-born calves, were ana- lyzed. The results showed that no significant differences were found in conception rate among different groups （P〉0.05）; there was no significant difference in dystocia rate between the A and C groups （P〉0.05）, but the dystocia rate in the B group was significantly different from those in the A and C groups （P〈0.05）. There were positive correlations between calf forehead width, chest circumference and the dys- tocia rate.

Nutritional Analysis and Physiological Activity Evaluation of Pneumatophorus japonicus Sauce

The nutritional composition of Pneumatophorus japonicus sauce was analyzed and compared with other fish sauces. The sodium chloride content of the sample Pneumatophorus japonicus sauce was greater than 25%. The total soluble nitrogen content was greater than 2.1 g/100mL, and the amino nitrogen content was greater than 1.2 g/100mL. The mineral content was abundant in the Pneumatophorus japonicus sauce, the calcium content and the magnesium content were 391.50±0.03 and 375.00±0.02 mg/L respectively, much higher than those in other samples. The iron content and the zinc content were 22.30±0.04 and 2.80±0.03 mg/L, respectively. The content of free amino acids was 5 552.17 mg/100mL, the content of essential amino acids was 2 666.00 mg/100mL,The taurine content was 113.05 mg/100mL. The antioxidant activity of the fermentation broth was 90.48%, while the TCA soluble peptide content was 29.04 mg/mL. Pneumatophorus japonicus sauce is nutritionally rich and has special physiological activity.

Synthesis of Some More New Fluorinated 1,2,4-triazino[3,4-b][1,3,4]Thiadiazolonesand Their Molluscicidal Against Selective Snails - Part I

Some more new fluorinated 1,2,4-triasino[3,4-b][1,3,4]thiadiazolones （6-9 and 11-16） have been synthesized from cyclocondensation of 5-hydrazino-2-（4＇-fluorophenyl）-1,3,4-thiadiazole with bifunctional oxygen and halogen compounds. Structures of the products have been deduced from their elemental, chemical and spectral data （UV, IIL 1H/13C NMR （nuclear magnetic resonance） and mass）. All the new compounds evaluated as molluscicidal against towards snails which responsible for Bilharziasis diseases.

The Relationships Between Milk Constituents and Various Milk Properties in Anatolian Buffaloes

The objectives of this study were to investigate the relationships among milk composition, renneting time, urea concentration, acidity, density and pH of Anatolian Buffaloes＇ milk. As a total of 115 individual milk samples from 53 Anatolian buffalo cows that calved in 2004 and 2005 on days of their lactations 30 ± 15, 60 ± 15, 90 ±15, 120 ± 15, 150 ± 15, 180± 15, 210 ±15, 240 ± 15 and 270 ± 15 in 8 units of lhkpmar village were collected in morning milkings in June, September, December and March. Samples were analysed for total dry matter （TDM）, fat, protein, ash, density, pH, acidity, renneting time and urea content. Data were classified according to the following environmental factors： lactation stages： （30 ± 15, 60 i 15, 90 ±15 days）： 2 （120±15, 150 ± 15, 180 ± 15 days）： 3 （210 ± 15, 240 ± 15, 270± 15 days）; calving year： 1 （2004）, 2 （2005）; calving season： 1 （January-May）, 2 （September and October）; month of samples collection： 1 （June）, 2 （September）, 3 （December）, 4 （March）; lactation order： 1 and 2：1, 3 and 4：2, 5 and 6：3. Means and correlation coefficients for the characteristics investigated were calculated. There were negative significant correlations between daily milk yield with TDM, fat and protein percentages, and between pH and all of the milk constituents. Density reduces as TDM, fat and protein contents increase. Relationships between density and coagulation time with milk yield and pH were not significant. Relationships between milk urea concentration with none of milk constituents, milk yield, density, pH and titratable acidity were not significant statistically. It was concluded that genetic selection has to be directed towards increasing fat, protein and total not fat dry matter yields. Under selection programs in which milk yield is taken into consideration, fat and protein yields also increase, but fat and protein concentrations decrease.

Study of Estrus Cow Serum （ECS） in Maturation Media on in vitro Maturation Rate of Bovine Oocytes

The objective of this research was to study the effect of ECS on cumulus expansion and rate of nucleus maturation of bovine oocytes. Media maturation were used： （1） TCM 199 ＋ FCS 10%; （2） TCM 199 ＋ FCS 10% ＋ ECS 5%; （3） TCM 199 ＋ FCS 10% ＋ ECS 7, 5%; （4） TCM 199 ＋ FCS 10% ＋ ECS 10%; （5） TCM 199 ＋ ECS 10%. Supplementation of ECS had significantly difference （P 〈 0.05） on expansion of cumulus cells and rate of nucleus maturation. Supplementation of ECS 5% was the best result in expanded cumulus cells and metaphase II rate： 82% and 72% respectively. It was concluded that medium of TCM 199 ＋ FCS 10%o ＋ ECS 5% was the best maturation medium

Effects of High-Pressure Technology on the Functional Properties of Milk and Fermented Milk Products

HPP （high pressure processing） is one of the novel technologies to produce microbiologically safe food. HPP is a non-thermal food processing method, wherein the food is subjected to a very high pressure ranging between 100-800 MPa in order to prevent undesirable chemical and microbiological reactions, and hence, prolong the shelf-life. HPP is also called as ＂high hydrostatic processing, ultra-high pressure processing or isostatic processing＂. In dairy products, HHP has the potential to modify the functional properties of proteins, polysaccharides and alter biochemical reactions without significantly affecting the nutritional and sensory properties. HPP treatment induces significant changes in milk components particularly in proteins （whey proteins and caseins）, as well as on their applicability in innovative dairy productions. HPP influences technological properties of various milk products such as firmness, water-holding capacity of the gel and network structure, cheese yield, rennet coagulation time and ripening.

Analysis of the Mechanical Behavior of Bovine Hoof Horn Using Digital Image Correlation

In this study Digital Image Correlation (DIC) was adopted to evaluate the mechanical properties of bovine hoof horn. Using specific process, tensile specimens comprised of Zones 4 and 5 of the sole were excised from the claws of the front and rear hooves of a mature Black Angus cow. After extraction the specimens were conditioned to obtain horn with relative moisture content of either 0% (dry) or 100% (wet) and then subjected to uniaxial tension to failure. The average elastic modulus (E) of all "dry" and "wet" hoof horn was 2870 ± 460 MPa and 102 ± 34 MPa respectively. Similarly, the average proportional limit stress (σ) was 29.0 ± 4.8 MPa and 1.4 ± 0.3 MPa respectively. Although the mechanical properties were dependent on distance from the dermal-epidermal junction, the influence of moisture on mechanical behavior was far greater.

Development of an Anaerobic Digestion Unit for Biogas Production from Cow Dung Substrate

An anaerobic digestion unit for producing biogas from cow dung in the rural communities was designed, fabricated and tested for performance, durability and throughput. The major components of the digester included the substrate holding tank, tank cover, agitator, debris collector, inlet and outlet pipes, gas reception tank, hose and heat source. The digester is a vertical cylindrical tank with an inlet pipe for the introduction of substrate and an outlet pipe to collect the digested substrate. An agitator is incorporated inside the digester to break scum on the substrate and create uniform temperature profile in the digester while a pressure gauge was fitted to the gas outlet valve to measure the gas pressure in the tank. The agitator shaft is extended outside to be driven by an electric motor through belt and pulley system. The criteria considered in the design of the digester included air tightness of the system, mesophilic and thermophilic temperature, nature and type of substrate used, substrate retention period, number of crank turns per minute and volumetric capacity of the digestion tank. Other considerations included the desire to make the digestion tank and gas reception tank of galvanized steel to ensure good quality of the product and the need for a strong structural support to ensure structural stability of the system. After construction and assembly, the biogas digestion unit was tested with 40 kg of cow dung diluted with 80 kg of water and subjected to a retention period to make a substrate （slurry） of 10 % total solid （TS）. Daily gas yield was determined; gas pressure in the tank was measured by the pressure gauge, while the ambient temperature was taken at five hours interval. Results showed that a cumulative gas yield of 0.415 litres after 22 d retention period at average substrate temperature and pH of 29 ℃ and 6.2, respectively. The digester has a substrate holding capacity of 330.8 litres and a production cost of $375 with all the construction materials being available locally.

Effects of Feeding Siamese neem leaves and Zanthoxylum Pods, on Dry Matter Intake, Dry Matter Digestibility, Milk Production and Composition in Thai Holstein Dairy Cows, Fed Rice Straw as Fiber Source

The plant secondary metabolites （PSM） are highly sought compounds for use as an alternative to conventionally used feed additives in animal production these days; Siamese neem leaf （Azadirachta indica A. Juss. var. siamenses Valeton） and Zanthoxylum pods （Zanthoxylum piperatum） are known to contain numerous such compounds.The objectives of this study were to determine effects of feeding Siamese neem leaf and Zanthoxylum pods as feed additives on dry matter intake （DMI）, dry matter digestibility （DMD） and milk production and milk composition. Lactating Thai Holstein cows （n = 8） were arranged in two replicates of 4 x 4 Latin square designs, housed in individual stall, treatments consisted of Siamese neem 0.5 and 1.5 g kgl dry matter （DM）, ZanthoxylumO. 1 and 0.5 g kg1 DM added to the total mixed ration （TMR）. TMRand drinking water were provided ad lib. There were no significant differences （P 〉 0.05） in DMI and crude protein （CP）, acid detergent fiber （ADF）, neutral detergent fiber （NDF） digestibility, milk production and composition among the treatments.However, significant difference （P 〈 0.05） was observed in dry matter （DM） and ether extract （EE） digestibility. Though statistically non-significant, Zanthoxylum at higher dose level tended to show low DMI （14.85 kg） in cows, while promotingmarginally higher milk （14.18 kg） production as compared with lower dose levelwhere DMI and milk production were 16.14 kg and 13.83 kg.This indicated that Zanthoxylum has a potential to improve feed digestion in dairy cows when used as additives.

Effect of Palm Oil By-pass Fat on Milk Composition of Early Lactation Holstein Cows Fed Whole Plant Corn Silage during Dry Season

The objective of this study was to investigate the effect of Palm Oil By-pass Fat （POBF） on milk composition of early lactation Holstein during dry season, 24 postpartum Holstein （1 d-14 d） were selected and align to two groups according to CRD experimental design; each group included 12 Holstein cows. The control received by-pass fat 0 g/h.d, the experimental group received by-pass fat 300 g/h.d. The results shown, DMI and yield of milk did not be affected with supplementation of by-pass fat, however, milk protein increased by 6.71%, milk fat increased by 8.16%, lactose increased by 0.22%, SNF increased by 1.98%, TS increased 4.08%; digestibility of diet protein decreased by 6.62%, digestibility of EE decreased by 7.96%, digestibility of NDF and ADL decreased by 1.36%, 8.97% respectively, digestibility of DM and OM decreased by 4.42, 3.07 percentage units; amount of C4-C17 milk fatty acids showed a decreased tendency, that of CIs-C22 showed an increased tendency, the milk CLA increased significantly （P 〈 0.01）. To sum up, supplementation of by-pass fat for early lactation Holstein is an important measure to balance energy and sustain milk yield and quality.

Modified Model of Bubble Formation in Non-Newtonian Fluids

Based on the comprehensive forces balance model, a modified model of the formation of a single bub-ble in non-Newtonian fluid under constant flowrate was developed by taking account of the effect of the ingoing gas through orifice as well as its variation on the radial expansion of bubble. The modified model involves the radial expansion equation of bubble surface and the forces balance equation in vertical direction of the bubble respec-tively. The shape variation of bubbles formed in polyacrylamide (PAM) aqueous solutions under various conditions was predicted numerically. The practical formation of bubbles was real-time visualized and recorded by a CCD camera and a computer by means of a special laser image measurement system. Results show that the predicted shapes of the bubbles by the present model agree well with experimental observation.

Identification and expression analysis of bovine ANGPTL gene family

Encoded by seven genes, angiopoietin-like （ANGPTL） family members structurally similar to the angiogenic regulating factor angiopoietin are known to possess biological activities in angiogenesis and metabolism. Here we reports for the first time the identification and expression analysis of all the seven members of bovine ANGPTL gene family, which were designated bANGPTL1 to bANGPTL7 in order. The seven bANGPTL genes consist of 4-9 exons, span 3800M-3000 bp and are located on different chromosomes. The deduced amino acid sequences of the members all possess an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain, both characteristics of angiopoietins. Phylogenetic analysis showed that the 32 identified ANGPTL homologs from 9 species could be classified into two major groups. Real-time quantitative PCR （Q-PCR） analysis revealed that the bANGPTL family members have different expression patterns. This study will be helpful for investigation on the biological role of the bANGPTL family in this economically important species. Furthermore, it provides an insight into the molecular evolution of the emerging ANGPTL family