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反义人IL-10和反义病毒性IL-10抗鼻咽癌细胞成瘤效应的研究 被引量:1
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作者 陶剑平 杨琨 +1 位作者 朱振宇 林学颜 《中国免疫学杂志》 CAS CSCD 北大核心 2002年第8期543-545,549,共4页
目的:探讨反义人IL-10和反义病毒性IL-10抑制鼻咽癌细胞成瘤的效果。方法:扩增及克隆hIL-10 cDNA和 vIL-10基因,构建反义hIL-10和反义vIL-10双价真核表达载体pcDNA3/AS hIL-10+AS vIL-10,转染鼻咽癌细胞株SUNE。将转染阳性的SUNE细胞克... 目的:探讨反义人IL-10和反义病毒性IL-10抑制鼻咽癌细胞成瘤的效果。方法:扩增及克隆hIL-10 cDNA和 vIL-10基因,构建反义hIL-10和反义vIL-10双价真核表达载体pcDNA3/AS hIL-10+AS vIL-10,转染鼻咽癌细胞株SUNE。将转染阳性的SUNE细胞克隆和未转染的SUNE细胞接种SCID小鼠和经健康人外周血白细胞免疫重建的SCID小鼠(hu-PBL-SCID鼠),观察成瘤效应。结果:ELISA法测得未转染SUNE细胞培养上清液中IL-10和vIL-10的总含量为242±23pg/ml,而转染阳性的SUNE细胞培养上清液中hIL-10和vIL-10的总含量仅为22±6pg/ml。在SCID鼠体内,vIL-10和hIL-10的表达与否,并不影响鼻咽癌细胞的成瘤活性,但在hu-PBL-SCID鼠体内,抑制vIL-10和hIL-10的表达则可明显抑制鼻咽癌细胞的成瘤作用。结论:反义人IL-10和反义病毒性IL-10可显著抑制鼻咽癌细胞在血hu-PBL-SCID鼠体内的成瘤作用,鼻咽癌组织表达vIL-10和hIL-10的确与鼻咽癌免疫耐受有关。 展开更多
关键词 反义人IL-10 反义病毒性IL-10 鼻咽癌 成瘤效应
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Functionalized Gold Nanorods for Tumor Imaging and Targeted Therapy 被引量:3
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作者 Chen Gui Da-xiang Cui 《Clinical oncology and cancer researeh》 CAS CSCD 2012年第4期221-233,共13页
Gold nanorods,as an emerging noble metal nanomaterial with unique properties,have become the new exciting focus of theoretical and experimental studies in the past few years.The structure and function of gold nanorods... Gold nanorods,as an emerging noble metal nanomaterial with unique properties,have become the new exciting focus of theoretical and experimental studies in the past few years.The structure and function of gold nanorods,especially their biocompatibility, optical property,and photothermal effects,have been attracting more and more attention.Gold nanorods exhibit great potential in applications such as tumor molecular imaging and photothermal therapy.In this article,we review some of the main advances made over the past few years in the application of gold nanorods in surface functionalization,molecular imaging,and photothermal therapy. We also explore other prospective applications and discuss the corresponding concepts,issues,approaches,and challenges,with the aim of stimulating broader interest in gold nanorod-based nanotechnology and improving its practical application. 展开更多
关键词 gold nanorods surface functionalization TUMOR molecular imaging photothermal therapy
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