Newly developed black garlic had made a sensational debut with a strong anti-tumor potency proved by the mouse tumor model in 2006 from our laboratory. At that time few researches on it have been conducted due to less...Newly developed black garlic had made a sensational debut with a strong anti-tumor potency proved by the mouse tumor model in 2006 from our laboratory. At that time few researches on it have been conducted due to less popularity of the products even among the researching field. Since then some of the bio-functional works of the black garlic gradually appeared along with gaining of its popularity. Representative bio-activities reported up to now are： （1） enhancement of anti-tumor potency in the mouse tumor model; （2） immune system enforcement, and suppressive act against allergic symptoms caused by decreasing IL-4 cytokine production; （3） bacteria killing potency; （4） anti-oxidant activity; （5） reduction of carcinogenic potency; （6） lowering cholesterol level and preventive work against obesity; （7） controlling high blood pressure; （8） beneficial effect against diabetics; （9） regenerative effect to the burned skin （Clinical study case） et al. On the black garlic business （marketing）, the Aomori Black Garlic Union （Association） had been established in Aomori Prefecture to offer the citizen high quality, high safety and high security black garlic （products） aiming at contribution for health improvement. All union members are now exerting themselves to the most to attain the high quality production of the black garlic. The quality proving certification seal will be issued to the products that passed the quality approval test by the third party inspections to place it on the their products. This system is now effectively working to guarantee the garlic quality and differentiate the Union member＇s products from others at the markets.展开更多
OBJECTIVE To analyze the effects of cryoablation on the mice bearing Rm-I prostate cancer through detecting tumor angiogenesis and cancer cell proliferation in the mice after cryoablation, and to explore the effects o...OBJECTIVE To analyze the effects of cryoablation on the mice bearing Rm-I prostate cancer through detecting tumor angiogenesis and cancer cell proliferation in the mice after cryoablation, and to explore the effects of cryoablation on vascular endothelium growth factor （VEGF）, Ki67 protein expression and microvessel density （MVD） in the mice bearing prostate cancer. METHODS Sixty Rm-1 mouse models of prostate cancer were established. Experimental mice were randomized into 2 groups： the cryoablation group （n = 30） and the control group （n = 30）. After file therap）4 tumor tissues of the mice in group A and B were obtained at day 0 （without cryoablation）, 1st, 3rd, 5th, 7th, 14th day, respectivelj6 after cryoablation, and the expressions of MVD, VEGF and Ki67 proteins were detected at the same time points. RESULTS The expressions of MVD, VEGF and Ki67 proteins in group A were decreased. The lowest values of the factors were detected on the 3rd day after cryoablation, and increased slowly after that. The expressions of MVD, VEGF and Ki67 proteins in the control group were not changed. Significant changes of the expressions of MVD, VEGF and Ki67 proteins in the group A were found at different time points. Correlation analysis suggested a positive correlation between the expressions of VEGF and MVD proteins （r = 0.8793）, a positive correlation between the expressions of Ki67 and MVD proteins （r = 0.7614）, and a positive correlation between the expressions of VEGF and ki67 proteins （r = 0.6921）. CONCLUSION After argon-helium cryoablation treatment for the mice bearing prostate cancer, the expressions of MVD, VEGF and Ki67 proteins in local tumor were reduced on the 1st day. The lowest values of the factors were detected on the 3rd day after cryoablation, and then increased after that. Cryoablation combined with other modalities of treatment may effectively improve the treatment effects of cryoablation for prostate cancer.展开更多
Ultrasmall FeCo-graphitic carbon shell nanocrystals （FeCo/GC） are promising multifunctional materials capable of highly efficient drug delivery in vitro and magnetic resonance imaging in vivo. In this work, we demon...Ultrasmall FeCo-graphitic carbon shell nanocrystals （FeCo/GC） are promising multifunctional materials capable of highly efficient drug delivery in vitro and magnetic resonance imaging in vivo. In this work, we demonstrate the use of FeCo/GC for highly effective cancer therapy through combined drug delivery, tumor-selective near-infrared photothermal therapy, and cancer imaging of a 4T1 syngeneic breast cancer model. The graphitic carbon shell of the ~4 nm FeCo/GC readily loads doxorubicin （DOX） via π-π stacking and absorbs near-infrared light giving photothermal heating. When used for cancer treatment, intravenously administrated FeCo/GC-DOX led to complete tumor regression in 45% of mice when combined with 20 min of near-infrared laser irradiation selectively heating the tumor to 43-45 ℃. In addition, the use of FeCo/GC-DOX results in reduced systemic toxicity compared with free DOX and appears to be safe in mice monitored for over 1 yr. FeCo/GC-DOX is shown to be a highly integrated nanoparticle system for synergistic cancer therapy leading to tumor regression of a highly aggressive tumor model.展开更多
文摘Newly developed black garlic had made a sensational debut with a strong anti-tumor potency proved by the mouse tumor model in 2006 from our laboratory. At that time few researches on it have been conducted due to less popularity of the products even among the researching field. Since then some of the bio-functional works of the black garlic gradually appeared along with gaining of its popularity. Representative bio-activities reported up to now are： （1） enhancement of anti-tumor potency in the mouse tumor model; （2） immune system enforcement, and suppressive act against allergic symptoms caused by decreasing IL-4 cytokine production; （3） bacteria killing potency; （4） anti-oxidant activity; （5） reduction of carcinogenic potency; （6） lowering cholesterol level and preventive work against obesity; （7） controlling high blood pressure; （8） beneficial effect against diabetics; （9） regenerative effect to the burned skin （Clinical study case） et al. On the black garlic business （marketing）, the Aomori Black Garlic Union （Association） had been established in Aomori Prefecture to offer the citizen high quality, high safety and high security black garlic （products） aiming at contribution for health improvement. All union members are now exerting themselves to the most to attain the high quality production of the black garlic. The quality proving certification seal will be issued to the products that passed the quality approval test by the third party inspections to place it on the their products. This system is now effectively working to guarantee the garlic quality and differentiate the Union member＇s products from others at the markets.
文摘OBJECTIVE To analyze the effects of cryoablation on the mice bearing Rm-I prostate cancer through detecting tumor angiogenesis and cancer cell proliferation in the mice after cryoablation, and to explore the effects of cryoablation on vascular endothelium growth factor （VEGF）, Ki67 protein expression and microvessel density （MVD） in the mice bearing prostate cancer. METHODS Sixty Rm-1 mouse models of prostate cancer were established. Experimental mice were randomized into 2 groups： the cryoablation group （n = 30） and the control group （n = 30）. After file therap）4 tumor tissues of the mice in group A and B were obtained at day 0 （without cryoablation）, 1st, 3rd, 5th, 7th, 14th day, respectivelj6 after cryoablation, and the expressions of MVD, VEGF and Ki67 proteins were detected at the same time points. RESULTS The expressions of MVD, VEGF and Ki67 proteins in group A were decreased. The lowest values of the factors were detected on the 3rd day after cryoablation, and increased slowly after that. The expressions of MVD, VEGF and Ki67 proteins in the control group were not changed. Significant changes of the expressions of MVD, VEGF and Ki67 proteins in the group A were found at different time points. Correlation analysis suggested a positive correlation between the expressions of VEGF and MVD proteins （r = 0.8793）, a positive correlation between the expressions of Ki67 and MVD proteins （r = 0.7614）, and a positive correlation between the expressions of VEGF and ki67 proteins （r = 0.6921）. CONCLUSION After argon-helium cryoablation treatment for the mice bearing prostate cancer, the expressions of MVD, VEGF and Ki67 proteins in local tumor were reduced on the 1st day. The lowest values of the factors were detected on the 3rd day after cryoablation, and then increased after that. Cryoablation combined with other modalities of treatment may effectively improve the treatment effects of cryoablation for prostate cancer.
文摘Ultrasmall FeCo-graphitic carbon shell nanocrystals （FeCo/GC） are promising multifunctional materials capable of highly efficient drug delivery in vitro and magnetic resonance imaging in vivo. In this work, we demonstrate the use of FeCo/GC for highly effective cancer therapy through combined drug delivery, tumor-selective near-infrared photothermal therapy, and cancer imaging of a 4T1 syngeneic breast cancer model. The graphitic carbon shell of the ~4 nm FeCo/GC readily loads doxorubicin （DOX） via π-π stacking and absorbs near-infrared light giving photothermal heating. When used for cancer treatment, intravenously administrated FeCo/GC-DOX led to complete tumor regression in 45% of mice when combined with 20 min of near-infrared laser irradiation selectively heating the tumor to 43-45 ℃. In addition, the use of FeCo/GC-DOX results in reduced systemic toxicity compared with free DOX and appears to be safe in mice monitored for over 1 yr. FeCo/GC-DOX is shown to be a highly integrated nanoparticle system for synergistic cancer therapy leading to tumor regression of a highly aggressive tumor model.
