Objective： By establishing the indirect contact co-culture system, we studied the in vitro condition for MAPCs differentiating into epidermal cells and the transformation of MAPCs into epidermal cell phenotype. Meth...Objective： By establishing the indirect contact co-culture system, we studied the in vitro condition for MAPCs differentiating into epidermal cells and the transformation of MAPCs into epidermal cell phenotype. Methods： Cell culture insert membrane was used for substitute basal membrane and MAPCs, fibroblast cells （FCs） and mixture of MAPCs and epidermal cells and FCs were separately implanted into 2 sides of it. PKH26 was used to label cloned MAPCs; type IV collagen rapid adhering method was used to isolate and culture the skin epidermal cells from l-day-old SD rat. Results： Part of the MAPCs transformed into cells expressing keratin in the presence of peripheral epithelia and FCs. Type Ⅳ collagen rapid adhering method successfully selected rats＇ epidermal stem cells. The mixture of the 2 kinds of cells or indirect culture might promote the differentiation through mesenchymal factors secreted by dermis FC. Conclusion： We were the first to have established the in vitro model of MAPCs differentiation into epidermal cells, in which MAPCs were transformed into epithelium-like cells.展开更多
This system can not only monitor the video signals from 16TV cameras at 10 different spots simultaneously, but also telecontrol the 16 cameras. The hardware in the system is simple, applicable and reliable, because in...This system can not only monitor the video signals from 16TV cameras at 10 different spots simultaneously, but also telecontrol the 16 cameras. The hardware in the system is simple, applicable and reliable, because intelligent components have been adopted and many functions can be achieved by software. Even if the code rates deviate from the designed value (300 baud/s) in 50 times, the remote signals can still be exactly received by the code rate measuring with software. The elash among remote signals from multiple spots is completely eliminated by checking their priorities with software.展开更多
Fibroblast growth factors (FGFs) can be classified as secretory (FGFI-10 and FGF15-23) or intracellular non-secretory forms (FGF 11-14). Secretory forms of FGF and their receptors are best known for their regulatory r...Fibroblast growth factors (FGFs) can be classified as secretory (FGFI-10 and FGF15-23) or intracellular non-secretory forms (FGF 11-14). Secretory forms of FGF and their receptors are best known for their regulatory roles in cell growth, differentiation and morphogenesis in the early stages of neural development. However, the functions of intracellular FGFs remain to be ex- plored. FGF12 and FGF14 are found to interact with voltage-gated sodium channels, and regulate the channel activity in neu- rons. FGF13 is expressed in primary sensory neurons, and is colocalized with sodium channels at the nodes of Ranvier along the myelinated afferent fibers. FGF13 is also expressed in cerebral cortical neurons during the late developmental stage. A re- cent study showed that FGFI3 is a microtubule-stabilizing protein required for regulating the neuronal development in the cerebral cortex. Thus, non-secretory forms of FGF appear to have important roles in the brain, and it would be interesting to further investigate the functions of intracellular FGFs in the nervous system and in neural diseases.展开更多
基金Supported by the National Natural Science Foundation of China(30600651)the Cooperation Foundation for Overseas Young Scientists (30428001)
文摘Objective： By establishing the indirect contact co-culture system, we studied the in vitro condition for MAPCs differentiating into epidermal cells and the transformation of MAPCs into epidermal cell phenotype. Methods： Cell culture insert membrane was used for substitute basal membrane and MAPCs, fibroblast cells （FCs） and mixture of MAPCs and epidermal cells and FCs were separately implanted into 2 sides of it. PKH26 was used to label cloned MAPCs; type IV collagen rapid adhering method was used to isolate and culture the skin epidermal cells from l-day-old SD rat. Results： Part of the MAPCs transformed into cells expressing keratin in the presence of peripheral epithelia and FCs. Type Ⅳ collagen rapid adhering method successfully selected rats＇ epidermal stem cells. The mixture of the 2 kinds of cells or indirect culture might promote the differentiation through mesenchymal factors secreted by dermis FC. Conclusion： We were the first to have established the in vitro model of MAPCs differentiation into epidermal cells, in which MAPCs were transformed into epithelium-like cells.
文摘This system can not only monitor the video signals from 16TV cameras at 10 different spots simultaneously, but also telecontrol the 16 cameras. The hardware in the system is simple, applicable and reliable, because intelligent components have been adopted and many functions can be achieved by software. Even if the code rates deviate from the designed value (300 baud/s) in 50 times, the remote signals can still be exactly received by the code rate measuring with software. The elash among remote signals from multiple spots is completely eliminated by checking their priorities with software.
基金supported by the National Natural Science Foundation of China (Grant Nos. 31130066 and 30630029)National Basic Research Program of China (Grant Nos. 2011CBA00400 and 2009CB522005)Strategic Priority Research Program (B) of Chinese Academy of Sciences(Grant No. XDB02010200)
文摘Fibroblast growth factors (FGFs) can be classified as secretory (FGFI-10 and FGF15-23) or intracellular non-secretory forms (FGF 11-14). Secretory forms of FGF and their receptors are best known for their regulatory roles in cell growth, differentiation and morphogenesis in the early stages of neural development. However, the functions of intracellular FGFs remain to be ex- plored. FGF12 and FGF14 are found to interact with voltage-gated sodium channels, and regulate the channel activity in neu- rons. FGF13 is expressed in primary sensory neurons, and is colocalized with sodium channels at the nodes of Ranvier along the myelinated afferent fibers. FGF13 is also expressed in cerebral cortical neurons during the late developmental stage. A re- cent study showed that FGFI3 is a microtubule-stabilizing protein required for regulating the neuronal development in the cerebral cortex. Thus, non-secretory forms of FGF appear to have important roles in the brain, and it would be interesting to further investigate the functions of intracellular FGFs in the nervous system and in neural diseases.
