[Objective] The research aimed to discuss the effects of rosiglitazone and serum on the expressions of PPARα and PPARγ genes in the induced differentiation process of pig preadipocyte.[Method] The pig preadipocyte w...[Objective] The research aimed to discuss the effects of rosiglitazone and serum on the expressions of PPARα and PPARγ genes in the induced differentiation process of pig preadipocyte.[Method] The pig preadipocyte was separated by using the collagenase digestion method.Three kinds of different differentiation culture solutions were used to induce the differentiation of pig preadipocyte.The oil red O staining extraction method was used to contrast the influences of different differentiation culture solutions on the variation of cellular fat content in the differentiation process.Moreover,the variation trends of PPARα and PPARγ expressions in the cellular differentiation process in the different differentiation culture solutions were detected by the real-time quantification PCR.[Result] The cellular fat accumulation was the fastest in MII which contained rosiglitazone and was the slowest in MI which didn＇t contain rosiglitazone.Rosiglitazone could significantly increase the expression of PPARγ gene（P0.01）,but had the certain inhibition effect on the expression of PPARα gene,which wasn＇t significant.The serum had the extremely significant up-regulation effect on the expression of PPARγ gene（P0.01）,but had the extremely significant down-regulation effect on the expression of PPARα gene（P0.01）.[Conclusion] Rosiglitazone could greatly promote the expression of PPARγ gene,which increased the cellular fat deposition.Maybe the activator of PPARγ gene existed in the serum,and the inhibitor of PPARα gene existed simultaneously.展开更多
Long-chain acyl coenzyme A synthetase(ACSL) is a member of the synthetase family encoded by a multigene family;it plays an important role in the absorption and transport of fatty acid.Here we review the roles of ACSL ...Long-chain acyl coenzyme A synthetase(ACSL) is a member of the synthetase family encoded by a multigene family;it plays an important role in the absorption and transport of fatty acid.Here we review the roles of ACSL in the regulating absorption and transport of fatty acid,as well as the connection between ACSL and some metabolic diseases.展开更多
Phaeodactylum tricornutum is a diatom that is rich in lipids. Recently, it has received much attention as a feedstock for biodiesel production. Nitrogen deficiency is widely known to increase the content of neutral li...Phaeodactylum tricornutum is a diatom that is rich in lipids. Recently, it has received much attention as a feedstock for biodiesel production. Nitrogen deficiency is widely known to increase the content of neutral lipids （mainly triacylglycerides, or TAGs） of microalgae, including P. tricornutum, but the mechanism is unclear. In this study, we deciphered the correlations between TAG content and nine key enzymatic genes involved in lipid synthesis in P. tricornuturn. After being cultured under nitrogen-free conditions for 0, 4, 24, 48, 72, 120, and 168 h, the TAG contents ofP. tricornutum cells were assayed and the transcript abundances of the target genes were monitored by quantitative real-time PCR. The results show that the abundances of four target gene transcripts （LACS3, G3PDH2, G3PDH3, and G3PDH5） were positively correlated with TAG content, indicating that these genes may be involved in TAG synthesis in P. tricornutum. The findings improve our understanding of the metabolic network and regulation of lipid synthesis and will guide the future genetic improvement of the TAG content ofP. tricornutum.展开更多
We examined the effect of linseed supplementation in finishing diet on intramuscular fat level and fatty acid composition of three bovine muscles （longissimus dorsi-LD, semitendinosus-ST, psoas major-PM）, total of s...We examined the effect of linseed supplementation in finishing diet on intramuscular fat level and fatty acid composition of three bovine muscles （longissimus dorsi-LD, semitendinosus-ST, psoas major-PM）, total of sixty-two young bulls, from six different genotypes Angus （A）, Charolais （CH）, Holstein-Friesian （H）, Hungarian Grey （HG）, Hungarian Simmental （HS）, Charolais x Hungarian Grey F1 （CHxHG）. The results showed that A bulls had the highest intramuscular fat （IM） level （3.76）, particularly in PM （4.74）, followed by HG （3.40）, crossbred HG （3.05）, CH （2.33）, HS （2.21）, with the lowest level for H （2.11） bulls. IMF for LD （2.87） and ST （1.77） muscles were significantly lower than PM （3.60） for all groups. SFA was significantly higher for PM than the other two, due to the high level oflM. The highest MUFA was detected in LD, significantly different from others. ST contained the highest PUFA （12.99）, followed by LD （10.30） and PM （10.19）. The n-6 fatty acids were affected either by breed, or by muscle type. The linoleic acid was the highest in muscle samples of Holstein bulls except for PM, while the lowest was in A bulls in all cases. The same tendency can be seen in all cases for long chain n-6 fatty acids. The overall mean of n-3 fatty acids varied among genotypes from 1.36 to 1.80%. The highest level was measured in ST of CH and H （2.19）, whereas the lowest one in LD of HG （1.22）. Significant differences among genotypes were shown only for PM. The level of n-3 series long chain fatty acids was the highest for ST, for LD and PM in H, CH and HS, respectively. The ratio of n-6/n-3 ranged from 4.48 to 6.22 and was significantly less favorable in H than in A bulls. The highest CLA level was detected in all cases in HG bulls.展开更多
Host genes involved in lipid metabolism are differentially affected during the early stages of hepatitis C virus （HCV） infection. Here we demonstrate that artificial up-regulation of fatty acid biosynthesis has a po...Host genes involved in lipid metabolism are differentially affected during the early stages of hepatitis C virus （HCV） infection. Here we demonstrate that artificial up-regulation of fatty acid biosynthesis has a positive effect on the replication of the HCV full-length replicon when cells were treated with nystatin. Conversely, the HCV RNA replication was decreased when fatty acid biosynthesis was inhibited with 25-hydroxycholesterol and PDMP（D-threo-l-phenyl-2-decanoylamino-3- morpholino-l-propanol）. In agreement with these results, the expression level of GlcT-l（ceramide glucosyltransferase）, a host glucosyltransferase in the first step of GSL （glycosphingolipid） biosynthesis, was found to be closely associated with the expression and replication of HCV RNA. On the other hand, the viral RNA can also activate GlcT-1 in the early stage of viral RNA transfection in vitro. To identify viral factors that are responsible for GlcT-1 activation, we constructed ten stable Vero cell lines that express individual HCV proteins. Based on the analyses of these cell lines and transient transfection assay of the GlcT-1 promoter regions, we conclude that HCV proteins, especially NS5A and NS5B, have positive effects on the expression of GlcT-1. It is possible that NS5A and NS5B stimulate transcription factor（s） to activate the expression of GlcT-1 by increasing its transcription level展开更多
基金Supported by Ministry of Science & Technology International Cooper-ation Project(2011DFB30340)the Earmarked Fund for China Agriculture Research System(CARS-36)the Project of Animal Breeding from Sichuan Bureau of Science & Technology(2006-YZGG-15)~~
文摘[Objective] The research aimed to discuss the effects of rosiglitazone and serum on the expressions of PPARα and PPARγ genes in the induced differentiation process of pig preadipocyte.[Method] The pig preadipocyte was separated by using the collagenase digestion method.Three kinds of different differentiation culture solutions were used to induce the differentiation of pig preadipocyte.The oil red O staining extraction method was used to contrast the influences of different differentiation culture solutions on the variation of cellular fat content in the differentiation process.Moreover,the variation trends of PPARα and PPARγ expressions in the cellular differentiation process in the different differentiation culture solutions were detected by the real-time quantification PCR.[Result] The cellular fat accumulation was the fastest in MII which contained rosiglitazone and was the slowest in MI which didn＇t contain rosiglitazone.Rosiglitazone could significantly increase the expression of PPARγ gene（P0.01）,but had the certain inhibition effect on the expression of PPARα gene,which wasn＇t significant.The serum had the extremely significant up-regulation effect on the expression of PPARγ gene（P0.01）,but had the extremely significant down-regulation effect on the expression of PPARα gene（P0.01）.[Conclusion] Rosiglitazone could greatly promote the expression of PPARγ gene,which increased the cellular fat deposition.Maybe the activator of PPARγ gene existed in the serum,and the inhibitor of PPARα gene existed simultaneously.
基金Supported by the National Natural Science Foundation of China(81373465)
文摘Long-chain acyl coenzyme A synthetase(ACSL) is a member of the synthetase family encoded by a multigene family;it plays an important role in the absorption and transport of fatty acid.Here we review the roles of ACSL in the regulating absorption and transport of fatty acid,as well as the connection between ACSL and some metabolic diseases.
基金Supported by the National Basic Research Program of China(973 Program)(No.2011CB200901)the National Key Technology Research and Development Program(No.2011BAD14B01)+1 种基金the Promotive Research Fund for Excellent Young and Middle-Aged Scientists of Shandong Province(No.BS2010SW037)the National Natural Science Foundation of China(No.31270408)
文摘Phaeodactylum tricornutum is a diatom that is rich in lipids. Recently, it has received much attention as a feedstock for biodiesel production. Nitrogen deficiency is widely known to increase the content of neutral lipids （mainly triacylglycerides, or TAGs） of microalgae, including P. tricornutum, but the mechanism is unclear. In this study, we deciphered the correlations between TAG content and nine key enzymatic genes involved in lipid synthesis in P. tricornuturn. After being cultured under nitrogen-free conditions for 0, 4, 24, 48, 72, 120, and 168 h, the TAG contents ofP. tricornutum cells were assayed and the transcript abundances of the target genes were monitored by quantitative real-time PCR. The results show that the abundances of four target gene transcripts （LACS3, G3PDH2, G3PDH3, and G3PDH5） were positively correlated with TAG content, indicating that these genes may be involved in TAG synthesis in P. tricornutum. The findings improve our understanding of the metabolic network and regulation of lipid synthesis and will guide the future genetic improvement of the TAG content ofP. tricornutum.
文摘We examined the effect of linseed supplementation in finishing diet on intramuscular fat level and fatty acid composition of three bovine muscles （longissimus dorsi-LD, semitendinosus-ST, psoas major-PM）, total of sixty-two young bulls, from six different genotypes Angus （A）, Charolais （CH）, Holstein-Friesian （H）, Hungarian Grey （HG）, Hungarian Simmental （HS）, Charolais x Hungarian Grey F1 （CHxHG）. The results showed that A bulls had the highest intramuscular fat （IM） level （3.76）, particularly in PM （4.74）, followed by HG （3.40）, crossbred HG （3.05）, CH （2.33）, HS （2.21）, with the lowest level for H （2.11） bulls. IMF for LD （2.87） and ST （1.77） muscles were significantly lower than PM （3.60） for all groups. SFA was significantly higher for PM than the other two, due to the high level oflM. The highest MUFA was detected in LD, significantly different from others. ST contained the highest PUFA （12.99）, followed by LD （10.30） and PM （10.19）. The n-6 fatty acids were affected either by breed, or by muscle type. The linoleic acid was the highest in muscle samples of Holstein bulls except for PM, while the lowest was in A bulls in all cases. The same tendency can be seen in all cases for long chain n-6 fatty acids. The overall mean of n-3 fatty acids varied among genotypes from 1.36 to 1.80%. The highest level was measured in ST of CH and H （2.19）, whereas the lowest one in LD of HG （1.22）. Significant differences among genotypes were shown only for PM. The level of n-3 series long chain fatty acids was the highest for ST, for LD and PM in H, CH and HS, respectively. The ratio of n-6/n-3 ranged from 4.48 to 6.22 and was significantly less favorable in H than in A bulls. The highest CLA level was detected in all cases in HG bulls.
基金the National"973"Program of China(No.2011CB504800)
文摘Host genes involved in lipid metabolism are differentially affected during the early stages of hepatitis C virus （HCV） infection. Here we demonstrate that artificial up-regulation of fatty acid biosynthesis has a positive effect on the replication of the HCV full-length replicon when cells were treated with nystatin. Conversely, the HCV RNA replication was decreased when fatty acid biosynthesis was inhibited with 25-hydroxycholesterol and PDMP（D-threo-l-phenyl-2-decanoylamino-3- morpholino-l-propanol）. In agreement with these results, the expression level of GlcT-l（ceramide glucosyltransferase）, a host glucosyltransferase in the first step of GSL （glycosphingolipid） biosynthesis, was found to be closely associated with the expression and replication of HCV RNA. On the other hand, the viral RNA can also activate GlcT-1 in the early stage of viral RNA transfection in vitro. To identify viral factors that are responsible for GlcT-1 activation, we constructed ten stable Vero cell lines that express individual HCV proteins. Based on the analyses of these cell lines and transient transfection assay of the GlcT-1 promoter regions, we conclude that HCV proteins, especially NS5A and NS5B, have positive effects on the expression of GlcT-1. It is possible that NS5A and NS5B stimulate transcription factor（s） to activate the expression of GlcT-1 by increasing its transcription level
