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不同品系大鼠皮下脂肪源干细胞诱导分化能力的比较 被引量:2
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作者 曲戎梅 姚红卫 +4 位作者 戴景兴 陈白虹 张艳玲 林来兴妹 原林 《实用医学杂志》 CAS 北大核心 2011年第7期1151-1153,共3页
目的:对比SD大鼠和Wistar大鼠皮下脂肪源干细胞(adipose-derived stem cells,ADSCs)体外诱导分化能力,为ADSCs的进一步应用提供实验依据。方法:SD大鼠和Wistar大鼠各6只取腹股沟脂肪垫,培养扩增ADSCs,相差显微镜下观察两个品系来源的AD... 目的:对比SD大鼠和Wistar大鼠皮下脂肪源干细胞(adipose-derived stem cells,ADSCs)体外诱导分化能力,为ADSCs的进一步应用提供实验依据。方法:SD大鼠和Wistar大鼠各6只取腹股沟脂肪垫,培养扩增ADSCs,相差显微镜下观察两个品系来源的ADSCs的形态。用第4代细胞进行成骨和成脂诱导,分别用茜素红和油红O染色观察向成骨细胞分化后的矿化结节和向脂肪细胞分化后的脂质沉积。结果:两个品系来源的ADSCs在细胞形态上没有显著区别,都能进行成骨和成脂诱导分化。结论:SD大鼠和Wistar大鼠腹股沟脂肪垫来源的ADSCs诱导分化能力差异无显著性。 展开更多
关键词 干细胞 分化 腹股沟脂肪 成脂肪诱导 诱导
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逆转录病毒介导增强型绿色荧光蛋白基因在大鼠骨髓间充质干细胞中的表达 被引量:3
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作者 曾智凤 易著文 +4 位作者 何庆南 吴小川 党西强 何小解 奠双红 《世界华人消化杂志》 CAS 北大核心 2008年第9期1008-1011,共4页
目的:观察逆转录病毒介导绿色荧光蛋白基因在SD大鼠骨髓间充质干细胞(bone marrow stromal cells,BMSCs)中的表达,为干细胞移植机制的研究提供基础.方法:采用密度梯度离心法分离培养BMSCs,诱导培养液诱导其向成脂肪方向分化,行细胞表面... 目的:观察逆转录病毒介导绿色荧光蛋白基因在SD大鼠骨髓间充质干细胞(bone marrow stromal cells,BMSCs)中的表达,为干细胞移植机制的研究提供基础.方法:采用密度梯度离心法分离培养BMSCs,诱导培养液诱导其向成脂肪方向分化,行细胞表面抗原鉴定,及油红O染色评价细胞成脂肪情况.在此基础上,采用逆转录病毒pLEGFP-N1对细胞进行荧光蛋白标记.观察细胞形态学改变荧光表达的时间与强度,计算转染率.结果:细胞扩增迅速,形态良好,纯度较高,经诱导后细胞内可见脂滴.逆转录病毒载体pLEGFP-N1成功标记SD大鼠骨髓间充质干细胞,并对4 wk体外培养进行了良好的标记.结论:逆转录病毒pLEGFP-N1转染效率高,转染成功的BMSCs可以长期稳定表达目的基因,是一种理想的病毒载体. 展开更多
关键词 骨髓基质干细胞 成脂肪诱导 增强型绿色 荧光蛋白 逆转录病毒 转染
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Reprogramming mature terminally differentiated adipocytes to induced pluripotent stem cells 被引量:1
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作者 Tao Nie Wei Deng +9 位作者 Xuefei Gao Wei Sun Xiaoyan Hui Hong Song Dajiang Qin Aimin Xu Peng Li Pentao Liu Liangxue Lai Donghai Wu 《Science Bulletin》 SCIE EI CAS CSCD 2015年第20期1752-1758,共7页
Mature adipocytes are terminally differentiated somatic cells. Here, we report the successful generation of induced pluripotent stem (iPS) cells from mouse mature adipocytes by forced expression of six transcription... Mature adipocytes are terminally differentiated somatic cells. Here, we report the successful generation of induced pluripotent stem (iPS) cells from mouse mature adipocytes by forced expression of six transcription factors (Oct4, Sox2, c-Myc, Klf4, Rarγ, and Lrh1) with a piggyBac transposon-based strategy. The resulting iPS cells were pluripotent as evidenced by the fact that they stained positive for alkaline phosphatase, expressed high levels of key pluripotency markers including Oct4, Nanog, and SSEA1, and remained pluripotent on a 2i media. In vitro differen- tiation of the iPS cells showed that the cell derivatives of all three germ layers could be readily obtained through forma- tion of embryoid bodies. Most importantly, these adipocyte- derived iPS cells were capable of producing chimera with high frequencies when reintroduced into early-stage em- bryos and transmitted through the germ line. This study demonstrates that the new six-factor reprogramming tech- nology facilitates the reset of the terminally differentiated adipocytes to the ground state of pluripotency, enabling us to fully explore the potential of mature adipocytes as a viable cell source for regenerative medicine. 展开更多
关键词 ADIPOCYTE Induced pluripotent stem cell PIGGYBAC
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Therapeutic effect of osteogenically induced adipose derived stem cells on vascular deprivation-induced osteonecrosis of the femoral head in rabbits 被引量:2
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作者 Abuclusaimi Aimaiti Yilixiafi Saiwulaiti +3 位作者 Maimaitiming Saiyiti WANG Yun-hai CUI Lei Aihemaitijiang Yusufu 《Chinese Journal of Traumatology》 CAS 2011年第4期215-220,共6页
Objective: To explore the therapeutic ef- fect of osteogenically induced adipose-derived stem cells (ADSCs) on vascular deprivation-induced osteonecrosis of the femoral head (ONFH) in rabbit model. Methods: Vas... Objective: To explore the therapeutic ef- fect of osteogenically induced adipose-derived stem cells (ADSCs) on vascular deprivation-induced osteonecrosis of the femoral head (ONFH) in rabbit model. Methods: Vascular deprivation-induced ONFH was established by intramuscular injection of methylpre- dnisolone, and vascular occlusion of the capital femoral epiphysis by electrocoagulation in adult New Zealand white rabbits. Eight weeks afLer the establishment of vascular dep- rivation-induced ONFH, animals were randomly divided into three equal groups. In Group A (control), no therapy was given. In Group B, core decompression was performed by drilling a hole (1.2 mm in diameter) from the outer cortex 2.5 cm distal to the proximal end of the greater trochanter. In Group C, 1 x 107 osteogenically induced ADSCs were resus- pended in 0.5 ml PBS, and then injected directly into the femoral head. Femoral head specimens were obtained at postoperative 8 weeks. The bone formation and three-di- mensional microstructure of the femoral head was evaluated by micro-computed tomography scans (u-CT). Immunohistochemical analysis was performed to detect the expression ofosteocalcin. Angiogenesis and repair of the femoral head were observed histologically. Results: In trabecular bone at the proximal femur region, the trabecular volume was higher in Group C (130.70 mm3± 4.33 mm3) than that in Groups A (101.07 mm3±7.76 mm3) and B (107.89 mm3±8.6g mm3, P〈0.01). Bone volume was significantly increased in Group C (40.09 mm3±6.35 mm3) than in Groups A (29.65 mm3±4.61 mm3) and B (31.80 mm3± 4.01 mm3, P〈0.01). The trabecular number was higher in Groups C (1.58±0.25) than other two groups (1.15±0.18, 1.16± 0.21, P〈0.01). Bone mineral density showed statistically significant difference between Groups C and A or B (375.38± 23.06) mg HA/ccm, vs (313.73 ± 19.30) mg HA/ccm and (316.09± 16.45) mg HA/ccm, P〈0.01). Histological examination indicated that there was more new bone formation in Group C than in other groups. Conclusion: Treatment with autologous osteogenically induced ADSCs transplantation results in an enhanced osteogenesis and microstructure of the vascular deprivation-induced osteonecrosis in rabbits. 展开更多
关键词 Adipose tissue Stem cells OSTEONECROSIS OSTEOGENESIS Transplantation autologus
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