A full habituated cell line C_ 20hi was screened from 2,4_D dependent line (C_ 20D) of Catharanthus roseus (L.) G. Don. The investigation involved the cell growth, ajmalicine production and enzyme activity related t...A full habituated cell line C_ 20hi was screened from 2,4_D dependent line (C_ 20D) of Catharanthus roseus (L.) G. Don. The investigation involved the cell growth, ajmalicine production and enzyme activity related to indole alkaloid biosynthesis in both cell lines. These results indicated that C_ 20hi cells grew faster than C_ 20D cells, and average ajmalicine content in C_ 20hi cells was 18.4 times more than that in C_ 20D when cultured in the production medium. In the growth medium, average ajmalicine content in C_ 20hi cells was 31.9 times more than that in C_ 20D cells, while the cell growth has no obvious difference. The comparison of enzyme activities in C_ 20hi and C_ 20D cells indicated that tryptophan decarboxylase (TDC), strictosidine synthase (SSS) and geraniol_10_dehydrogenase (G10H) activities have no close relation to ajmalicine accumulation, although the activities of these enzymes were higher when cells were cultured in the production medium than in the growth medium. The C_ 20hi cells are relatively stable in five years of culture.展开更多
Objective: We established a transplantation tumor model of human osteosarcoma in chick embryo, studied its morphological and biological characteristics, and observed its dynamic process of angiogenesis induction so th...Objective: We established a transplantation tumor model of human osteosarcoma in chick embryo, studied its morphological and biological characteristics, and observed its dynamic process of angiogenesis induction so that a simple and practical model can be provided for studying osteosarcoma. Methods: Human osteosarcoma cells at different concentrations were inoculated in chorioallantoic membrane (CAM) of chick embryos at different embryonic ages to observe the factors affecting the survival of the transplanted osteosarcoma in chick embryo, growth characteristics of the transplantation tumor, and the morphological characteristics and biological characteristics of the transplantation tumor. Results: The transplantation tumor model of human osteosarcoma in chick embryo was successfully established. It was found that the transplantation tumor was easy to grow and it showed strong angiogenesis-inducing effects. Under the light microscope, the transplantation tumor showed a similar tissue structure to human osteosarcoma. Conclusion: It is feasible to establish a transplantation tumor model of human osteosarcoma in chick embryo. The model can be easily duplicated with a simple operation, which provides a useful animal model for studying osteosarcoma.展开更多
Sustainable forage production is one of the most important factors in livestock production system. Smallholder livestock production system is a part of agricultural practice in Indonesia. Limited land owned by farmers...Sustainable forage production is one of the most important factors in livestock production system. Smallholder livestock production system is a part of agricultural practice in Indonesia. Limited land owned by farmers was dominantly cultivated for food crops, and very small part of it used for cultivating forage plants. This leads to restriction of smallholder livestock development, because no more land available for forage production. Integrated forage production system by planting forage plants and food crops or trees in the same area becomes a considerable cropping system that widely practiced by the farmers in Indonesia. Some grasses and legumes have morphological advantages overcoming growth condition under shading. These growth characteristics are benefit in designing compatible system components for enhanced productivity in tree-pasture systems. Four potential shade-tolerant creeping forages that were combined with 2 levels of nitrogen application were tested using factorial completely randomized design 3 replicates. The first factor was species of creeping forages （stoloniferous）： BH = Brachiaria humidicola, PN = Paspalum notatum, AC - Axonopus compressus and, AP = Arachis pintoi. The second factor was nitrogen （urea） fertilizer： A = without N fertilizer and B = with N fertilizer （300 kg Urea/ha）. The observations included the growth rates of plant length, plant length, number of leaves, herbages yield （shoot）, root dry weight, senescence, total N-shoot, N-soil and N-uptake. The results showed that growth characteristics and productivity of creeping forage plants was significantly differed by the species. Most species showed an increase in growth trend, except P. notatum. A. compressus significantly showed as the best performance species compared to other, in terms of growth rates, plant length, number of leaves, herbage yield （shoot） and root dry weight, N-uptake and N-shoot. This was lbllowed by B. humidicola, P. notatum, and A. pintoi. Based on previous study, A. compressus found as one of the native species in most tree system. It indicated its superior growth compared to other species tested. However, it is needed further research to observe the compatibility of each species in tree-pasture systems.展开更多
Video synopsis is an effective and innovative way to produce short video abstraction for huge video archives,while keeping the dynamic characteristic of activities in the original video.Abnormal activity,as the critic...Video synopsis is an effective and innovative way to produce short video abstraction for huge video archives,while keeping the dynamic characteristic of activities in the original video.Abnormal activity,as the critical event,is always the main concern in video surveillance context.However,in traditional video synopsis,all the normal and abnormal activities are condensed together equally,which can make the synopsis video confused and worthless.In addition,the traditional video synopsis methods always neglect redundancy in the content domain.To solve the above-mentioned issues,a novel video synopsis method is proposed based on abnormal activity detection and key observation selection.In the proposed algorithm,activities are classified into normal and abnormal ones based on the sparse reconstruction cost from an atomically learned activity dictionary.And key observation selection using the minimum description length principle is conducted for eliminating content redundancy in normal activity.Experiments conducted in publicly available datasets demonstrate that the proposed approach can effectively generate satisfying synopsis videos.展开更多
Objective: To elucidate the effects of exogenous basic fibroblast growth factor ( bFGF ) on biological characteristics of rat osteoblasts cultured in vitro.Methods: The osteoblasts isolated from a Sprague-Dawley rat a...Objective: To elucidate the effects of exogenous basic fibroblast growth factor ( bFGF ) on biological characteristics of rat osteoblasts cultured in vitro.Methods: The osteoblasts isolated from a Sprague-Dawley rat and cultured in vitro were treated with different concentrations of bFGF (5-50 ng/ml) respectively. At 24 hours after treatment, the proliferating cell nuclear antigen was measured with immunocytochemistry, alkaline phosphatase ( ALP) activity was determined and the expression of transforming growth factor beta 1 (TGF-β1) was detected to observe the effects of bFGF on growth and differentiation of osteoblasts. Results: bFGF ( 5-50 ng/ml ) could obviously promote the growth of osteoblasts. The intracellular expression of TGF-β, mRNA increased significantly, but the intracellular ALP content decreased.Conclusions: bFGF can obviously stimulate the proliferation of osteoblasts and promote the synthesis of TGF-β1, but cannot promote the differentiation of osteoblasts.展开更多
文摘A full habituated cell line C_ 20hi was screened from 2,4_D dependent line (C_ 20D) of Catharanthus roseus (L.) G. Don. The investigation involved the cell growth, ajmalicine production and enzyme activity related to indole alkaloid biosynthesis in both cell lines. These results indicated that C_ 20hi cells grew faster than C_ 20D cells, and average ajmalicine content in C_ 20hi cells was 18.4 times more than that in C_ 20D when cultured in the production medium. In the growth medium, average ajmalicine content in C_ 20hi cells was 31.9 times more than that in C_ 20D cells, while the cell growth has no obvious difference. The comparison of enzyme activities in C_ 20hi and C_ 20D cells indicated that tryptophan decarboxylase (TDC), strictosidine synthase (SSS) and geraniol_10_dehydrogenase (G10H) activities have no close relation to ajmalicine accumulation, although the activities of these enzymes were higher when cells were cultured in the production medium than in the growth medium. The C_ 20hi cells are relatively stable in five years of culture.
文摘Objective: We established a transplantation tumor model of human osteosarcoma in chick embryo, studied its morphological and biological characteristics, and observed its dynamic process of angiogenesis induction so that a simple and practical model can be provided for studying osteosarcoma. Methods: Human osteosarcoma cells at different concentrations were inoculated in chorioallantoic membrane (CAM) of chick embryos at different embryonic ages to observe the factors affecting the survival of the transplanted osteosarcoma in chick embryo, growth characteristics of the transplantation tumor, and the morphological characteristics and biological characteristics of the transplantation tumor. Results: The transplantation tumor model of human osteosarcoma in chick embryo was successfully established. It was found that the transplantation tumor was easy to grow and it showed strong angiogenesis-inducing effects. Under the light microscope, the transplantation tumor showed a similar tissue structure to human osteosarcoma. Conclusion: It is feasible to establish a transplantation tumor model of human osteosarcoma in chick embryo. The model can be easily duplicated with a simple operation, which provides a useful animal model for studying osteosarcoma.
文摘Sustainable forage production is one of the most important factors in livestock production system. Smallholder livestock production system is a part of agricultural practice in Indonesia. Limited land owned by farmers was dominantly cultivated for food crops, and very small part of it used for cultivating forage plants. This leads to restriction of smallholder livestock development, because no more land available for forage production. Integrated forage production system by planting forage plants and food crops or trees in the same area becomes a considerable cropping system that widely practiced by the farmers in Indonesia. Some grasses and legumes have morphological advantages overcoming growth condition under shading. These growth characteristics are benefit in designing compatible system components for enhanced productivity in tree-pasture systems. Four potential shade-tolerant creeping forages that were combined with 2 levels of nitrogen application were tested using factorial completely randomized design 3 replicates. The first factor was species of creeping forages （stoloniferous）： BH = Brachiaria humidicola, PN = Paspalum notatum, AC - Axonopus compressus and, AP = Arachis pintoi. The second factor was nitrogen （urea） fertilizer： A = without N fertilizer and B = with N fertilizer （300 kg Urea/ha）. The observations included the growth rates of plant length, plant length, number of leaves, herbages yield （shoot）, root dry weight, senescence, total N-shoot, N-soil and N-uptake. The results showed that growth characteristics and productivity of creeping forage plants was significantly differed by the species. Most species showed an increase in growth trend, except P. notatum. A. compressus significantly showed as the best performance species compared to other, in terms of growth rates, plant length, number of leaves, herbage yield （shoot） and root dry weight, N-uptake and N-shoot. This was lbllowed by B. humidicola, P. notatum, and A. pintoi. Based on previous study, A. compressus found as one of the native species in most tree system. It indicated its superior growth compared to other species tested. However, it is needed further research to observe the compatibility of each species in tree-pasture systems.
基金Supported by the National Natural Science Foundation of China(No.61402023)Beijing Technology and Business' University Youth Fund(No.QNJJ2014-23)Beijing Natural Science Foundation(No.4162019)
文摘Video synopsis is an effective and innovative way to produce short video abstraction for huge video archives,while keeping the dynamic characteristic of activities in the original video.Abnormal activity,as the critical event,is always the main concern in video surveillance context.However,in traditional video synopsis,all the normal and abnormal activities are condensed together equally,which can make the synopsis video confused and worthless.In addition,the traditional video synopsis methods always neglect redundancy in the content domain.To solve the above-mentioned issues,a novel video synopsis method is proposed based on abnormal activity detection and key observation selection.In the proposed algorithm,activities are classified into normal and abnormal ones based on the sparse reconstruction cost from an atomically learned activity dictionary.And key observation selection using the minimum description length principle is conducted for eliminating content redundancy in normal activity.Experiments conducted in publicly available datasets demonstrate that the proposed approach can effectively generate satisfying synopsis videos.
文摘Objective: To elucidate the effects of exogenous basic fibroblast growth factor ( bFGF ) on biological characteristics of rat osteoblasts cultured in vitro.Methods: The osteoblasts isolated from a Sprague-Dawley rat and cultured in vitro were treated with different concentrations of bFGF (5-50 ng/ml) respectively. At 24 hours after treatment, the proliferating cell nuclear antigen was measured with immunocytochemistry, alkaline phosphatase ( ALP) activity was determined and the expression of transforming growth factor beta 1 (TGF-β1) was detected to observe the effects of bFGF on growth and differentiation of osteoblasts. Results: bFGF ( 5-50 ng/ml ) could obviously promote the growth of osteoblasts. The intracellular expression of TGF-β, mRNA increased significantly, but the intracellular ALP content decreased.Conclusions: bFGF can obviously stimulate the proliferation of osteoblasts and promote the synthesis of TGF-β1, but cannot promote the differentiation of osteoblasts.
