MC3T3-E1 osteoblasts were cultured on H2O2- modified and unmodified carbon/carbon （H-C/C and C/C） composites for one week in order to evaluate differences in cell adhesion, spreading, and proliferation. The results ...MC3T3-E1 osteoblasts were cultured on H2O2- modified and unmodified carbon/carbon （H-C/C and C/C） composites for one week in order to evaluate differences in cell adhesion, spreading, and proliferation. The results indicated a certain degree of enhancement in the cell adhesion capability of osteoblasts cultured on H-C/C samples. Cellu- lar morphologies after cell adhesion were observed via scan- ning electron microscopy （SEM）, which showed that the cells adhered more closely and spread more widely on the H-C/C sample surface. However, no cell appeared in several mul- tiple and continuous types of minor pores on both the C/C and H-C/C surfaces. In addition, two unique situations were observed on the H-C/C samples： an outline change of the osteoblasts was observed when the cells spread across some minor pores, and the cells entered and adhered well in some larger pores.展开更多
Objective： To investigate the effect of a new biomaterial combining calcium citrate and recombinant human bone morphogenetic protein-2 （rhBMP-2） on bone regeneration in a bone defect rabbit model. Methods： Totall...Objective： To investigate the effect of a new biomaterial combining calcium citrate and recombinant human bone morphogenetic protein-2 （rhBMP-2） on bone regeneration in a bone defect rabbit model. Methods： Totally 30 male New Zealand white rabbits were randomly and equally divided into calcium citraterhBMP-2 （CC-rhBMP-2） group and rhBMP-2 only group. Two 10 ram-long and 5 ram-deep bone defects were respectively created in the left and right femoral condyles of the rabbits. Subsequently 5 pellets of calcium citrate （10 mg） combined with rhBMP-2 （2 rag） or rhBMP-2 alone were implanted into the bone defects and compressed with cotton swab. Bone granules were obtained at 2, 4 and 6 weeks after procedure and received histological analysis. LSD t-test and a subsequent t-test were adopted for statistical analysis. Results： Histomorphometric analysis revealed newlyformed bones, and calcium citrate has been absorbed in the treatment group. The percent of newly formed bone area in femoral condyle in control group and CC-rhBMP-2 group was respectively 31.73%±1.26% vs 48.21%±2.37% at 2 weeks; 43.40%±1.65% vs 57.32%±1.47% at 4 weeks, and 51.32%±7.80% vs 66.74%±4.05% at 6 weeks （P〈0.05 for all）. At 2 weeks, mature cancellous bone was observed to be already formed in the treatment group. Conclusion： From this study, it can be concluded that calcium citrate combined with rhBMP-2 signifcantly enhances bone regeneration in bone defects. This synthetic gelatin matrix stimulates formation of new bone and bone marrow in the defect areas by releasing calcium ions.展开更多
Porous materials such as metal-organic frameworks(MOFs)with high theoretical volumetric gas uptake capacity are promising materials for gas storage and separation,but the structuring for practical applications is chal...Porous materials such as metal-organic frameworks(MOFs)with high theoretical volumetric gas uptake capacity are promising materials for gas storage and separation,but the structuring for practical applications is challenging.Herein,we report a general and feasible strategy to combine electrospinning with a phase conversion method to decorate polyacrylonitrile nanofibers(PAN NFs)with CuMOF(HKUST-1).The strategy is based on the combination of surface pretreatment of the NFs with Cu(OH)_(2) and a subsequent phase conversion into HKUST-1 crystals(PCHKUST-1).A significant higher loading of HKUST-1 in the PAN NF matrix was achieved by the phase conversion method compared with direct electrospinning of MOF slurries or insitu growth of MOF crystals on NFs.As a result,the hierarchical structured PC(phase conversion)-HKUST-1 NFs revealed the highest gravimetric storage capacity of 86 cm^(3) g^(-1)(STP)at 3500 kPa and 298 K for methane(CH_(4)),which is higher than other HKUST-1 NFs reported previously.The improved CH_(4) uptake can be explained by the high loading of HKUST-1 due to the high availability of Cu-ions localized on the surface of the NFs during the phase conversion process,resulting in high surface area and excellent gas access of the phase converted HKUST-1.Thus,the developed strategy of structuring MOFs could be of interest for the fabrication of tailor-made MOF NF architectures for other energy and environmental applications.展开更多
基金supported by the National Natural Science Foundation of China(51472203 and 51521061)the Natural Science Basic Research Plan in Shaanxi Province of China(2014JM6233)"111" project of china(B08040)
文摘MC3T3-E1 osteoblasts were cultured on H2O2- modified and unmodified carbon/carbon （H-C/C and C/C） composites for one week in order to evaluate differences in cell adhesion, spreading, and proliferation. The results indicated a certain degree of enhancement in the cell adhesion capability of osteoblasts cultured on H-C/C samples. Cellu- lar morphologies after cell adhesion were observed via scan- ning electron microscopy （SEM）, which showed that the cells adhered more closely and spread more widely on the H-C/C sample surface. However, no cell appeared in several mul- tiple and continuous types of minor pores on both the C/C and H-C/C surfaces. In addition, two unique situations were observed on the H-C/C samples： an outline change of the osteoblasts was observed when the cells spread across some minor pores, and the cells entered and adhered well in some larger pores.
文摘Objective： To investigate the effect of a new biomaterial combining calcium citrate and recombinant human bone morphogenetic protein-2 （rhBMP-2） on bone regeneration in a bone defect rabbit model. Methods： Totally 30 male New Zealand white rabbits were randomly and equally divided into calcium citraterhBMP-2 （CC-rhBMP-2） group and rhBMP-2 only group. Two 10 ram-long and 5 ram-deep bone defects were respectively created in the left and right femoral condyles of the rabbits. Subsequently 5 pellets of calcium citrate （10 mg） combined with rhBMP-2 （2 rag） or rhBMP-2 alone were implanted into the bone defects and compressed with cotton swab. Bone granules were obtained at 2, 4 and 6 weeks after procedure and received histological analysis. LSD t-test and a subsequent t-test were adopted for statistical analysis. Results： Histomorphometric analysis revealed newlyformed bones, and calcium citrate has been absorbed in the treatment group. The percent of newly formed bone area in femoral condyle in control group and CC-rhBMP-2 group was respectively 31.73%±1.26% vs 48.21%±2.37% at 2 weeks; 43.40%±1.65% vs 57.32%±1.47% at 4 weeks, and 51.32%±7.80% vs 66.74%±4.05% at 6 weeks （P〈0.05 for all）. At 2 weeks, mature cancellous bone was observed to be already formed in the treatment group. Conclusion： From this study, it can be concluded that calcium citrate combined with rhBMP-2 signifcantly enhances bone regeneration in bone defects. This synthetic gelatin matrix stimulates formation of new bone and bone marrow in the defect areas by releasing calcium ions.
基金supported by the Grande Solution Project“HiGradeGas”(48279)Innovation Fund Denmark,exploring NFs-based adsorbents for biogas upgrading and storage+1 种基金the Danish Research Council to provide funding to support fundamental research on electrospinning(8022-00237B)for investigating NFs structures for enzyme immobilization(6111-00232B)。
文摘Porous materials such as metal-organic frameworks(MOFs)with high theoretical volumetric gas uptake capacity are promising materials for gas storage and separation,but the structuring for practical applications is challenging.Herein,we report a general and feasible strategy to combine electrospinning with a phase conversion method to decorate polyacrylonitrile nanofibers(PAN NFs)with CuMOF(HKUST-1).The strategy is based on the combination of surface pretreatment of the NFs with Cu(OH)_(2) and a subsequent phase conversion into HKUST-1 crystals(PCHKUST-1).A significant higher loading of HKUST-1 in the PAN NF matrix was achieved by the phase conversion method compared with direct electrospinning of MOF slurries or insitu growth of MOF crystals on NFs.As a result,the hierarchical structured PC(phase conversion)-HKUST-1 NFs revealed the highest gravimetric storage capacity of 86 cm^(3) g^(-1)(STP)at 3500 kPa and 298 K for methane(CH_(4)),which is higher than other HKUST-1 NFs reported previously.The improved CH_(4) uptake can be explained by the high loading of HKUST-1 due to the high availability of Cu-ions localized on the surface of the NFs during the phase conversion process,resulting in high surface area and excellent gas access of the phase converted HKUST-1.Thus,the developed strategy of structuring MOFs could be of interest for the fabrication of tailor-made MOF NF architectures for other energy and environmental applications.
