林则徐戒烟方对地西泮自然戒断小鼠的治疗作用及初步机制研究

目的:探究林则徐戒烟方对地西泮自然戒断小鼠的治疗作用以及相关机制。方法:将40只KM小鼠,按照体质量随机分为正常对照组、模型组、戒烟方低剂量组、戒烟方中剂量组、戒烟方高剂量组,每组8只。正常对照组灌胃蒸馏水,其余各组连续灌服地西泮(2 mg/kg体质量)28 d,随后停止给予地西泮进行戒断,戒烟方低、中、高剂量组分别灌胃3 d林则徐戒烟方(7.8 g、15.5、31.0 g/kg),模型组和正常对照组灌胃等容积蒸馏水。灌胃3 d期间监测小鼠的一般状态。3 d后所有小鼠进行旷场实验,观察小鼠穿过中心格和周围格的数量、毛发梳理及直立次数。旷场实验结束后小鼠颈椎脱位法处死,取脑纹状体。高效液相-电化学方法检测小鼠纹状体中单胺类神经递质的含量。高效液相-荧光法检测小鼠纹状体中氨基酸类神经递质的含量。Western blotting方法检测纹状体中脑源性神经营养因子(BNDF)、磷酸化哺乳动物雷帕霉素靶蛋白(p-mTOR)和哺乳动物雷帕霉素靶蛋白(mTOR)表达变化。结果:小鼠停止给药地西泮3 d后出现体质量降低、探索欲望下降、运动减少,呈现出较明显的戒断反应。小鼠脑纹状体中多巴胺(DA)、5-羟色胺(5-HT)浓度和谷氨酸(Glu)/γ-氨基丁酸(GABA)明显降低,p-mTOR/mTOR和BDNF表达显著降低。林则徐戒烟方灌胃后,小鼠运动量增加,脑内DA和5-HT含量升高,抑制Glu/GABA和p-mTOR/mTOR的降低。结论:林则徐戒烟方可以改善地西泮戒断症状,其机制可能与通过调节纹状体内多种神经递质失衡,影响mTOR通路有关。

林则徐与中医药学

杰出的爱国主义者林则徐与中医药学有不解之缘。林则徐与何其伟合作研究“林文忠公戒烟方”,其主方“忌酸丸”和辅佐方“补正丸”的服法为递减递增法,此法后来风行各地,收获甚著。林则徐曾为陈念祖的“金匮要略浅注》作叙言。他亦和林昌彝等名中医交往甚密,同时他本人对中医学研究有素,深通中医摄生之道。

Markov Chain Model for Smoking Cessation Treatment

Probabilistic models are commonly used in computational medicine for diagnostics. Smoking cessation is an important issue of modern medicine. According to statistics about third part of male in global population are smokers. It is important to develop new approaches for smoking cessation treatment including methods of early diagnosis and development of individual treatment programs for each patient according to his or her physical peculiarities. One of the promising methods is computerized approach for tobacco treatment including electronic survey and computer data analysis. In this work we propose a probabilistic model based on Markov chain for estimation of patient behavior in the process on medical survey. This analysis can help to find out patient＇s individual characteristics and develop effective personal treatment program. Based on probabilistic model software was developed with aim to enhance diagnosis and developing individual smoking cessation treatment programs for each patient.