Bacterial community presumably plays an essential role in inhibiting pathogen colonization and maintaining the health of scallop larvae, but limiting data are available for Yesso scallop （Patinopecten yessoensisis Ja...Bacterial community presumably plays an essential role in inhibiting pathogen colonization and maintaining the health of scallop larvae, but limiting data are available for Yesso scallop （Patinopecten yessoensisis Jay, 1857） larval development stages. The aim of this study was to characterize and compare the bacterial communities associating with Yesso scallop larval development at fertilized egg S l, trochophora S2, D-shaped larvae S3, umbo larvae S4, and juvenile scallop S5 stages by Illumina high-throughput sequencing. Genomic DNA was extracted from the larvae and their associating baetera, and a gene segment covering V3-V4 region of 16S rRNA gene was amplified and sequenced using an Illumina Miseq sequencer. Overall, 106760 qualified sequences with an average length of 449 bp were obtained. Sequences were compared with those retrieved from 16S rRNA gene databases, and 4 phyla, 7 classes, 15 orders, 21 families, 31 genera were identified. Proteobacteria was predominant phylum, accounting for more than 99%, at all 5 larval development stages. At genus level, Pseudomonas was dominant at stages S1 （80.60%）, S2 （87.77%） and S5 （68.71%）, followed by Photobacterium （17.06%） and Aeromonas （1.64%） at stage S1, Serratia （6.94%）, Stenotrophomonas （3.08%） and Acinetobacter （1.2%） at stage S2, Shewanella （25.95%） and Pseudoalteromonas （4.57%） at stage S5. Moreover, genus Pseudoal- teromonas became dominant at stages S3 （44.85%） and S4 （56.02%）, followed by Photobacterium （29.82%）, Pseudomonas （11.86%）, Aliivibrio （8.60%） and Shewanella （3.39%） at stage S3, Pseudomonas （18.16%）, Aliivibrio （14.29%）, Shewanella （4.11%）, Psychro- monas （4.04%） and Psychrobacter （1.81%） at stage S4. From the results, we concluded that the bacterial community changed sig- nificantly at different development stages of Yesso Scallop larvae.展开更多
Recirculation is prohibited in many coal mining countries because of the fear that the re-use of return air would allow the build-up of air contaminants at the workings. The incorrect design and location of a booster ...Recirculation is prohibited in many coal mining countries because of the fear that the re-use of return air would allow the build-up of air contaminants at the workings. The incorrect design and location of a booster fan in any ventilation network can create unsafe condition due to recirculation. The current approach to investigating recirculation using simulation software requires manual effort which becomes tedious in a complex and a large network. An algorithm-based C++ program was designed to detect the recirculation in a booster fan ventilation networks. This program needed an input file prepared from output file generated by any ventilation simulator. This program created an output file for recirculation. This program demonstrated the strong capability to detect the recirculation in a sample network and a coal mine ventilation network. The outcomes of this program were documented in this paper.展开更多
This paper designs a set of ventilation and dust removal in coal mine test device and PLC monitoring system. The ventilation and dust removal test device has a set of ventilation and dust removal functions, can automa...This paper designs a set of ventilation and dust removal in coal mine test device and PLC monitoring system. The ventilation and dust removal test device has a set of ventilation and dust removal functions, can automatically monitor air volume, air pressure, resistance and other performance parameters of ventilator and precipitator. The PLC monitoring system can remotely start and stop the variable frequency speed control on the ventilation machine and dust collector, and it can real-time monitor operation state, such as the running current and the parameters of ventilator and dust collector. The paper simulate and realize computer remote centralized monitoring of underground ventilation for mine ventilation system. The actual application shows that： the system is stable and reliable, simple operation, strong scalability.展开更多
This study is aimed at using blade 3-D optimization to control corner flows in the high through-flow fan/booster of a high bypass ratio commercial turbofan engine. Two kinds of blade 3-D optimization, end-bending and ...This study is aimed at using blade 3-D optimization to control corner flows in the high through-flow fan/booster of a high bypass ratio commercial turbofan engine. Two kinds of blade 3-D optimization, end-bending and bow, are focused on. On account of the respective operation mode and environment, the approach to 3-D aerodynamic modeling of rotor blades is different from stator vanes. Based on the understanding of the mechanism of the corner flow and the consideration of intensity problem for rotors, this paper uses a variety of blade 3-D optimization approaches, such as loading distribution optimization, perturbation of departure angles and stacking-axis manipulation, which are suitable for rotors and stators respectively. The obtained 3-D blades and vanes can improve the corner flow features by end-bending and bow effects. The results of this study show that flows in corners of the fan/booster, such as the fan hub region, the tip and hub of the vanes of the booster, are very complex and dominated by 3-D effects. The secondary flows there are found to have a strong detrimental effect on the compressor performance. The effects of both end-bending and bow can improve the flow separation in corners, but the specific ways they work and application scope are somewhat different. Redesigning the blades via blade 3-D optimization to control the corner flow has effectively reduced the loss generation and improved the stall margin by a large amount.展开更多
基金financial support from Zhang Zidao Sland Group Co.,Ltd. for the project (99801214)
文摘Bacterial community presumably plays an essential role in inhibiting pathogen colonization and maintaining the health of scallop larvae, but limiting data are available for Yesso scallop （Patinopecten yessoensisis Jay, 1857） larval development stages. The aim of this study was to characterize and compare the bacterial communities associating with Yesso scallop larval development at fertilized egg S l, trochophora S2, D-shaped larvae S3, umbo larvae S4, and juvenile scallop S5 stages by Illumina high-throughput sequencing. Genomic DNA was extracted from the larvae and their associating baetera, and a gene segment covering V3-V4 region of 16S rRNA gene was amplified and sequenced using an Illumina Miseq sequencer. Overall, 106760 qualified sequences with an average length of 449 bp were obtained. Sequences were compared with those retrieved from 16S rRNA gene databases, and 4 phyla, 7 classes, 15 orders, 21 families, 31 genera were identified. Proteobacteria was predominant phylum, accounting for more than 99%, at all 5 larval development stages. At genus level, Pseudomonas was dominant at stages S1 （80.60%）, S2 （87.77%） and S5 （68.71%）, followed by Photobacterium （17.06%） and Aeromonas （1.64%） at stage S1, Serratia （6.94%）, Stenotrophomonas （3.08%） and Acinetobacter （1.2%） at stage S2, Shewanella （25.95%） and Pseudoalteromonas （4.57%） at stage S5. Moreover, genus Pseudoal- teromonas became dominant at stages S3 （44.85%） and S4 （56.02%）, followed by Photobacterium （29.82%）, Pseudomonas （11.86%）, Aliivibrio （8.60%） and Shewanella （3.39%） at stage S3, Pseudomonas （18.16%）, Aliivibrio （14.29%）, Shewanella （4.11%）, Psychro- monas （4.04%） and Psychrobacter （1.81%） at stage S4. From the results, we concluded that the bacterial community changed sig- nificantly at different development stages of Yesso Scallop larvae.
基金the National Institute for Occupational Safety and Health of USA for providing financial support for this project
文摘Recirculation is prohibited in many coal mining countries because of the fear that the re-use of return air would allow the build-up of air contaminants at the workings. The incorrect design and location of a booster fan in any ventilation network can create unsafe condition due to recirculation. The current approach to investigating recirculation using simulation software requires manual effort which becomes tedious in a complex and a large network. An algorithm-based C++ program was designed to detect the recirculation in a booster fan ventilation networks. This program needed an input file prepared from output file generated by any ventilation simulator. This program created an output file for recirculation. This program demonstrated the strong capability to detect the recirculation in a sample network and a coal mine ventilation network. The outcomes of this program were documented in this paper.
文摘This paper designs a set of ventilation and dust removal in coal mine test device and PLC monitoring system. The ventilation and dust removal test device has a set of ventilation and dust removal functions, can automatically monitor air volume, air pressure, resistance and other performance parameters of ventilator and precipitator. The PLC monitoring system can remotely start and stop the variable frequency speed control on the ventilation machine and dust collector, and it can real-time monitor operation state, such as the running current and the parameters of ventilator and dust collector. The paper simulate and realize computer remote centralized monitoring of underground ventilation for mine ventilation system. The actual application shows that： the system is stable and reliable, simple operation, strong scalability.
基金supported by National Natural Science Foundation of China (51006005,50736007)"Fan-Zhou" Youth Foundation(20100401)
文摘This study is aimed at using blade 3-D optimization to control corner flows in the high through-flow fan/booster of a high bypass ratio commercial turbofan engine. Two kinds of blade 3-D optimization, end-bending and bow, are focused on. On account of the respective operation mode and environment, the approach to 3-D aerodynamic modeling of rotor blades is different from stator vanes. Based on the understanding of the mechanism of the corner flow and the consideration of intensity problem for rotors, this paper uses a variety of blade 3-D optimization approaches, such as loading distribution optimization, perturbation of departure angles and stacking-axis manipulation, which are suitable for rotors and stators respectively. The obtained 3-D blades and vanes can improve the corner flow features by end-bending and bow effects. The results of this study show that flows in corners of the fan/booster, such as the fan hub region, the tip and hub of the vanes of the booster, are very complex and dominated by 3-D effects. The secondary flows there are found to have a strong detrimental effect on the compressor performance. The effects of both end-bending and bow can improve the flow separation in corners, but the specific ways they work and application scope are somewhat different. Redesigning the blades via blade 3-D optimization to control the corner flow has effectively reduced the loss generation and improved the stall margin by a large amount.
