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利用Illumina MiSeq测序分析手筑茯砖茶发酵及干燥阶段真菌群落多样性 被引量:6
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作者 陈梦娟 蒋立文 +2 位作者 徐元昊 周红丽 周辉 《食品科学》 EI CAS CSCD 北大核心 2020年第2期126-132,共7页
为了解手筑茯砖茶发酵与干燥过程中真菌种类变化,以发酵(0、4、8、12 d)及干燥阶段(17、22 d)的手筑茯砖茶样品为研究对象,应用Illumina MiSeq技术进行扩增测序,为手筑茯砖茶发酵及干燥阶段样品中真菌多样性研究奠定基础。结果表明:手... 为了解手筑茯砖茶发酵与干燥过程中真菌种类变化,以发酵(0、4、8、12 d)及干燥阶段(17、22 d)的手筑茯砖茶样品为研究对象,应用Illumina MiSeq技术进行扩增测序,为手筑茯砖茶发酵及干燥阶段样品中真菌多样性研究奠定基础。结果表明:手筑茯砖茶的发酵初期真菌群落结构存在较大差异,但随着发酵及干燥的进行真菌多样性整体呈下降趋势。测序及数据清理后共得到990522个有效的Tags,聚类后有186个可操作分类单元,注释到5个门、14个纲、29个目、47个科、65个属。其中0 d的真菌多样性最高,且相对丰度最高的为曲霉属(Aspergillus),达40.59%。其次为德巴利酵母属(Debaryomyces)、散囊菌属(Eurotium)和青霉属(Penicillium)等。而在4、8、12 d及17、22 d茯砖茶样品中,散囊菌属是绝对优势菌属,第4天时其相对丰度已达到97.27%,在这5组样品中其平均相对丰度高达98.81%。本研究采用Illumina MiSeq测序分析更准确、全面地反映手筑茯砖茶发酵与干燥过程中真菌群落结构和多样性动态变化,为手筑茯砖茶工艺的改进及机理的研究提供了新思路。 展开更多
关键词 手筑茯砖茶 Illumina MiSeq技术 真菌多样性
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桃源红茶手筑红茯砖加工技术
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作者 龚华春 周艮平 《中国园艺文摘》 2017年第1期223-224,共2页
阐述桃源红茶手筑红茯砖采用‘桃源大叶’3、4叶精制加工工艺技术:毛茶加工→毛茶拼配→足火提香→手筑定型→烘房发花。
关键词 红茶 手筑 茯砖 加工
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Seismic Enhancement of Existing Buildings by Means of Fiber Reinforced Concrete Diaphragms 被引量:1
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作者 Alessandra Marini Giovanni Plizzari Cristina Zanotti 《Journal of Civil Engineering and Architecture》 2010年第3期6-14,共9页
Floor diaphragms may provide an effective solution for reducing the seismic vulnerability of masonry buildings. Unfortunately, diaphragms are usually not present in historical building with wooden floors but often the... Floor diaphragms may provide an effective solution for reducing the seismic vulnerability of masonry buildings. Unfortunately, diaphragms are usually not present in historical building with wooden floors but often they are non present even in old R/C buildings where floors were made without shear reinforcement. A possible strengthening technique could be based on the application of a thin concrete plate reintbrced with a welded mesh. In order to reduce the thickness of the plate, some suitable solutions may be obtained by using Fiber Reinforced Concrete (FRC) since the minimum concrete cover is no longer required because the reinforcement (fibers) is spread all over the concrete matrix. The adoption of FRC floor diaphragms is proposed and discussed in this paper; the early results from a preliminary numerical study are analyzed in order to asses the feasibility of this new strengthening technique and better organize an experimental program that is currently in progress. 展开更多
关键词 Floor diaphragms seismic retrofit. Fiber Reinforced Concrete FE analysis
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Cost Control research in Construction Project Management
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作者 Danjing wang 《International Journal of Technology Management》 2013年第4期98-100,共3页
Construction project management as an important branch of management science has its own characteristics; it was known as an important aspect of project management in the 21 st century. Construction companies now face... Construction project management as an important branch of management science has its own characteristics; it was known as an important aspect of project management in the 21 st century. Construction companies now face a more complex competitive environment and more powerful competitors. In order to compete for the market and living space, Enterprises should be in a strategic height, cultivate long- term competitive advantage. Cost management is a new tool to enhance the competitiveness of enterprises, Popularization and application of Construction project management to the scientific development of the project management made the following three aspects urgent requirement. First. establish a scientific system for the construction project management. Construction Project Management knowledge system is the foundation and needs for Construction Project Management scientific and professional. 展开更多
关键词 Cost Control Construction Project Management Quality Control.
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Performance analysis of visual markers for indoor navigation systems
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作者 Gaetano C.LA DELFA Salvatore MONTELEONE +2 位作者 Vincenzo CATANIA Juan F.DE PAZ Javier BAJO 《Frontiers of Information Technology & Electronic Engineering》 SCIE EI CSCD 2016年第8期730-740,共11页
The massive diffusion of smartphones, the growing interest in wearable devices and the Internet of Things, and the exponential rise of location based services(LBSs) have made the problem of localization and navigati... The massive diffusion of smartphones, the growing interest in wearable devices and the Internet of Things, and the exponential rise of location based services(LBSs) have made the problem of localization and navigation inside buildings one of the most important technological challenges of recent years. Indoor positioning systems have a huge market in the retail sector and contextual advertising; in addition, they can be fundamental to increasing the quality of life for citizens if deployed inside public buildings such as hospitals, airports, and museums.Sometimes, in emergency situations, they can make the difference between life and death. Various approaches have been proposed in the literature. Recently, thanks to the high performance of smartphones' cameras, marker-less and marker-based computer vision approaches have been investigated. In a previous paper, we proposed a technique for indoor localization and navigation using both Bluetooth low energy(BLE) and a 2D visual marker system deployed into the floor. In this paper, we presented a qualitative performance evaluation of three 2D visual markers, Vuforia,Ar Uco marker, and AprilT ag, which are suitable for real-time applications. Our analysis focused on specific case study of visual markers placed onto the tiles, to improve the efficiency of our indoor localization and navigation approach by choosing the best visual marker system. 展开更多
关键词 Indoor localization Visual markers Computer vision
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