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蛋白质起始的DNA病毒复制机制
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作者 邢亚丽 司亚运 +3 位作者 袁弋 张清 姚勤 陈克平 《微生物学杂志》 CAS CSCD 2015年第3期65-70,共6页
DNA聚合酶在DNA合成过程中需要的引物包括RNA引物、DNA自我引物和蛋白质引物3种类型。新DNA链(如冈崎片段)的复制多是在DNA模板上合成一段RNA引物,细小病毒利用其基因组末端的反向末端重复序列(ITRs)自我折叠成DNA引物,而一些DNA、RNA... DNA聚合酶在DNA合成过程中需要的引物包括RNA引物、DNA自我引物和蛋白质引物3种类型。新DNA链(如冈崎片段)的复制多是在DNA模板上合成一段RNA引物,细小病毒利用其基因组末端的反向末端重复序列(ITRs)自我折叠成DNA引物,而一些DNA、RNA病毒及真菌质粒起始复制反应的引物则是蛋白质。以感染原核生物的噬菌体Phi29和真核DNA病毒腺病毒为例,从复制过程所涉及的蛋白质、对复制原点的识别、复制起始反应、新链的延伸、复制终止过程等方面详细阐述DNA病毒由蛋白质引发的复制机制,并对已商品化的Phi29 DNA聚合酶产品多重置换扩增及单细胞测序等的应用以及基于噬菌体Phi29蛋白质起始的最小复制系统体外扩增异源DNA等最新的应用研究作相关总结介绍。 展开更多
关键词 DNA病毒 蛋白质引物 复制起始 基因组扩增机制
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RNAi的技术研究进展 被引量:2
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作者 张金洲 项智锋 +1 位作者 李学斌 王清华 《河南科技学院学报》 2008年第3期57-60,共4页
RNA干扰是一些小的双链RNA可以高效、特异的阻断体内特定基因表达,促使mRNA降解,诱使细胞表现出特定基因缺失的表型,也是体内抵御外在感染的一种重要保护机制。本文对RNA i的发现过程及作用机理进行了综述,本技术已经成为最近生物医学... RNA干扰是一些小的双链RNA可以高效、特异的阻断体内特定基因表达,促使mRNA降解,诱使细胞表现出特定基因缺失的表型,也是体内抵御外在感染的一种重要保护机制。本文对RNA i的发现过程及作用机理进行了综述,本技术已经成为最近生物医学领域的焦点之一,将应用于基因功能和信号传导系统,上下游分子相互关系的研究可能为肿瘤基因治疗提供新的策略,在病毒性、寄生病原性、突变基因、癌基因和癌相关基因表达引起的疾病的基因治疗之中也有了新的突破。 展开更多
关键词 RNAI 基因功能 RNAI技术 基因沉默 扩增机制
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ESO-based decoupling control with multi-objective optimization for boiler-turbine unit 被引量:3
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作者 Zhu Jianzhong Wu Xiao Shen Jiong 《Journal of Southeast University(English Edition)》 EI CAS 2019年第1期64-71,共8页
A model-assistant extended state observer(MESO)-based decoupling control strategy is proposed for boiler-turbine units in the presence of unknown external disturbance and model-plant mismatch. For ease of implementati... A model-assistant extended state observer(MESO)-based decoupling control strategy is proposed for boiler-turbine units in the presence of unknown external disturbance and model-plant mismatch. For ease of implementation, the decoupling compensator is reduced to the proportion integration(PI) decoupler with the frequency domain analysis, where the decoupling error in collusion of uncertainties and disturbances can be estimated by the proposed MESO and then compensated. To decrease the sensitivity of the dynamic error for the decoupling control and fulfill various requirements of constraints, such as safety operation, energy conservation, emission reduction, etc., the plant is transmitted through a scheduled steady state region which is achieved from the optimized reference governor in advance. Simulation results show that the proposed control strategy can well suppress various disturbances including a decoupling error, and multi-objective optimization can meet multiple requirements with the premise of safety production. 展开更多
关键词 boiler-turbine unit extended state observer(ESO) decoupling control multi-objective optimization
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A scalable and efficient IPv4 address sharing approach in IPv6 transition scenarios 被引量:1
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作者 Guo-liang HAN Cong-xiao BAO Xing LI 《Frontiers of Information Technology & Electronic Engineering》 SCIE EI CSCD 2015年第8期634-645,共12页
IPv6 has been an inevitable trend with the depletion of the global IPv4 address space. However, new IPv6 users still need public IPv4 addresses to access global IPv4 users/resources, making it important for providers ... IPv6 has been an inevitable trend with the depletion of the global IPv4 address space. However, new IPv6 users still need public IPv4 addresses to access global IPv4 users/resources, making it important for providers to share scarce global IPv4 addresses effectively. There are two categories of solutions to the problem, carrier-grade NAT (CGN) and 'A+P' (each customer shaving the same IPv4 address is assigned an excluded port range). However, both of them have limitations. Specifically, CGN solutions are not scalable and can bring much complexity in managing customers in large-scale deployments, while A+P solutions are not flexible enough to meet dynamic port requirements. In this paper, we propose a hybrid mechanism to improve current solutions and have deployed it in the Tsinghua University Campus Network. The real traffic data shows that our mechanism can utilize limited IPv4 addresses efficiently without degrading the performance of applications on end hosts. Based on the enhanced mechanism, we propose a method to help service providers make address plans based on their own traffic patterns and actual requirements. 展开更多
关键词 IPv6 transition Carrier-grade NAT (CGN) A+P Address sharing Dynamic switching
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