Nanopipette based scanning probe technique is a versatile tool in non-contact imaging in biology.In addition to the topographic imaging,its capability of localized delivery of bio-active molecules is emerging.In this ...Nanopipette based scanning probe technique is a versatile tool in non-contact imaging in biology.In addition to the topographic imaging,its capability of localized delivery of bio-active molecules is emerging.In this mini review,we introduce the applications of nanopipette in single-cell researches with a focus on localized delivery.The working principles of three delivery modes including resistive pulse,pressure-driven flow,and electroosmotic flow-driven delivery are summarized and compared.Their applications in single-cell researches are reviewed.The current technical challenges in scanning ion conductance microscopy-based delivery,and their growing influence in medicine and pharmacologic researches are also discussed.展开更多
[Objective] The aim was to explore the morphological characteristics of ovum from Spodoptera exigua and Heliothis assulta.[Method]The ultra morphological characteristics of ovum was observed with scanning electron mic...[Objective] The aim was to explore the morphological characteristics of ovum from Spodoptera exigua and Heliothis assulta.[Method]The ultra morphological characteristics of ovum was observed with scanning electron microscope（SEM）.[Result]The ova from S.exigua and H.assulta are all spheroid,the former has net line decoration on surface,with some aeropyle distributed on the net line decoration;the micropyle is located in the center of polar and has plum flower-like engraving;the surface of chorion of the latter has the parallel crowns through two polar.Between the parallel crown,the surface of chorion has the decorated trapezium line,all crowns of second polar zone have breast-tuber that distributes in parallel at the same latitude in line,and there is no aeropyle distributing on chorion.[Conclusion] The ultra morphological characteristics of the ova from two Noctuidae species showed significant difference to each other,which enriched ontogenesis data and also provided reference for the accurate identification of two kinds of ova.展开更多
The recent developments of electron tomography（ET） based on transmission electron microscopy（TEM） and scanning transmission electron microscopy（STEM） in the field of materials science were introduced. The variou...The recent developments of electron tomography（ET） based on transmission electron microscopy（TEM） and scanning transmission electron microscopy（STEM） in the field of materials science were introduced. The various types of ET based on TEM as well as STEM were described in detail, which included bright-field（BF）-TEM tomography, dark-field（DF）-TEM tomography, weak-beam dark-field（WBDF）-TEM tomography, annular dark-field（ADF）-TEM tomography, energy-filtered transmission electron microscopy（EFTEM） tomography, high-angle annular dark-field（HAADF）-STEM tomography, ADF-STEM tomography, incoherent bright field（IBF）-STEM tomography, electron energy loss spectroscopy（EELS）-STEM tomography and X-ray energy dispersive spectrometry（XEDS）-STEM tomography, and so on. The optimized tilt series such as dual-axis tilt tomography, on-axis tilt tomography, conical tilt tomography and equally-sloped tomography（EST） were reported. The advanced reconstruction algorithms, such as discrete algebraic reconstruction technique（DART）, compressed sensing（CS） algorithm and EST were overviewed. At last, the development tendency of ET in materials science was presented.展开更多
基金support from the National Institute of General Medical Sciences(1R35G M147172-01).
文摘Nanopipette based scanning probe technique is a versatile tool in non-contact imaging in biology.In addition to the topographic imaging,its capability of localized delivery of bio-active molecules is emerging.In this mini review,we introduce the applications of nanopipette in single-cell researches with a focus on localized delivery.The working principles of three delivery modes including resistive pulse,pressure-driven flow,and electroosmotic flow-driven delivery are summarized and compared.Their applications in single-cell researches are reviewed.The current technical challenges in scanning ion conductance microscopy-based delivery,and their growing influence in medicine and pharmacologic researches are also discussed.
基金Supported by National Trades(Agriculture)Scientific Research Special Foundation(200803007)~~
文摘[Objective] The aim was to explore the morphological characteristics of ovum from Spodoptera exigua and Heliothis assulta.[Method]The ultra morphological characteristics of ovum was observed with scanning electron microscope（SEM）.[Result]The ova from S.exigua and H.assulta are all spheroid,the former has net line decoration on surface,with some aeropyle distributed on the net line decoration;the micropyle is located in the center of polar and has plum flower-like engraving;the surface of chorion of the latter has the parallel crowns through two polar.Between the parallel crown,the surface of chorion has the decorated trapezium line,all crowns of second polar zone have breast-tuber that distributes in parallel at the same latitude in line,and there is no aeropyle distributing on chorion.[Conclusion] The ultra morphological characteristics of the ova from two Noctuidae species showed significant difference to each other,which enriched ontogenesis data and also provided reference for the accurate identification of two kinds of ova.
基金Projects(51071125,51201135)supported by the National Natural Science Foundation of ChinaProject(B08040)supported by the Program of Introducing Talents of Discipline to Universities,China
文摘The recent developments of electron tomography（ET） based on transmission electron microscopy（TEM） and scanning transmission electron microscopy（STEM） in the field of materials science were introduced. The various types of ET based on TEM as well as STEM were described in detail, which included bright-field（BF）-TEM tomography, dark-field（DF）-TEM tomography, weak-beam dark-field（WBDF）-TEM tomography, annular dark-field（ADF）-TEM tomography, energy-filtered transmission electron microscopy（EFTEM） tomography, high-angle annular dark-field（HAADF）-STEM tomography, ADF-STEM tomography, incoherent bright field（IBF）-STEM tomography, electron energy loss spectroscopy（EELS）-STEM tomography and X-ray energy dispersive spectrometry（XEDS）-STEM tomography, and so on. The optimized tilt series such as dual-axis tilt tomography, on-axis tilt tomography, conical tilt tomography and equally-sloped tomography（EST） were reported. The advanced reconstruction algorithms, such as discrete algebraic reconstruction technique（DART）, compressed sensing（CS） algorithm and EST were overviewed. At last, the development tendency of ET in materials science was presented.
