This study aimed to achieve rapid detection of Pseudomonas syringae pv.tabaci,the pathogen of tobacco wildfire disease.The specific primers and probes for recombinase-aided amplification(RAA)were designed with HrpZ as...This study aimed to achieve rapid detection of Pseudomonas syringae pv.tabaci,the pathogen of tobacco wildfire disease.The specific primers and probes for recombinase-aided amplification(RAA)were designed with HrpZ as the target gene.RAA was then combined with the lateral flow dipstick(LFD)to establish a LFD-RAA-based rapid detection system for the pathogen.Furthermore,the detection performance of the established method was tested.The results showed that the LFDRAA method had high specificity.The amplification could be completed after 25 min of reaction at 39℃.The sensitivity of the established method reached 0.0001 ng/μL,which was superior to that of PCR detection.Moreover,the LFD-RAA method could quickly detect P.syringae pv.tabaci from tobacco leaves,demonstrating field applicability.To sum up,the LFD-RAA method established in this study can be applied in the rapid detection and early diagnosis of tobacco wildfire disease.展开更多
Objective To observe the cervical elasticity of healthy adult nulliparous women at different age groups and different stages of menstrual cycle with E-Cervix imaging technology.Methods A total of 218 healthy adult nul...Objective To observe the cervical elasticity of healthy adult nulliparous women at different age groups and different stages of menstrual cycle with E-Cervix imaging technology.Methods A total of 218 healthy adult nulliparous women who underwent transvaginal ultrasound examination for routine physical examination were retrospectively enrolled,including 103 in follicular phase,78 in ovulation phase and 37 in luteal phase.Cervical canal length(CL)and E-Cervix elasticity parameters were compared among different age groups and different stages of menstrual cycle,including elasticity contrast index(ECI),hardness ratio(HR),cervical internal and external orifice strain values(IOS and EOS)and IOS/EOS ratio.Results No significant difference of CL nor cervical elasticity parameters was detected among healthy adult nulliparous women at different age groups(all P>0.05).There were significant differences of ECI,HR and IOS among different menstrual cycle stages(all P<0.05),among which women in follicular phase had higher ECI and IOS but lower HR than those in luteal phase(all P<0.05).Conclusion No significant difference of cervical elasticity existed among healthy adult nulliparous women at different age groups.Meanwhile,cervical elasticity of healthy adult nulliparous women changed during menstrual cycle,in follicular phase had higher ECI and IOS but lower HR than in luteal phase.展开更多
Loop-mediated isothermal amplification(LAMP) is an amplification method developed by Notomi et al and has been applied successfully for the detection of many viruses.This paper introduced the current status of LAMP ...Loop-mediated isothermal amplification(LAMP) is an amplification method developed by Notomi et al and has been applied successfully for the detection of many viruses.This paper introduced the current status of LAMP and recent developments,and the method applying in the diagnosis of animal viruses in abroad.展开更多
Abstract Objective: To evaluate the diagnostic value of two-phase multidetector-row spiral CT threedimensional reconstruction technique in TNM staging of gastric cancer. Methods: In 29 patients with gastric carcinom...Abstract Objective: To evaluate the diagnostic value of two-phase multidetector-row spiral CT threedimensional reconstruction technique in TNM staging of gastric cancer. Methods: In 29 patients with gastric carcinoma pathologically conformed, plan scans were done firstly. Two-phase spiral CT was performed within one breathhold each. Distension of the stomach was achieved by intravenous application of anisodamine and effervescent granules. After bolus injection of contrast medium, scanning was performed in the arterial and venous phase, and the source images were thin reconstructed. The stomach to three-dimension analysis was constructed by volume rendering (VR) multiplanaz volume reconstruction (MPVR), shaded surface display (SSD) and CT virtual gastroscopy (CTVG) technique. In combination with the sources images, gastric tumour invasion and lymph node metastasis was assessed, and TNM staging was performed. Results: In 29 cases of gastric carcinoma, the sensitivity and specificity of two-phase multidetector-row spiral CT three-dimensional reconstruction technique in T1, T2, T3 and Ta staging, the sensitivity and specificity was 50% and 50%, 87.5% and 77.8%, 83.3% and 76.9% and 100% and 80% respectively. For the N staging, the sensitivity and specificity in No, N1, and N2 N3 was 83.3% and 71.4%, 87.5% and 77.8% and 81.8% and 75% respectively. The sensitivity and the specificity for M1 staging was 100%. Conclusion: The reconstruction technique in combination with 16-slices spiral-CT can perform TNM staging well and effectively guide the choice of the surgical procedures for gastric cancer.展开更多
The dimension lumber (45mm×90mm×3700mm) of plantation Chinese fir (Cunninghamia lanceolata (Lamb.) Hook.) was graded to four different classes as SS, No. 1, No.2 and No.3, according to national lumber ...The dimension lumber (45mm×90mm×3700mm) of plantation Chinese fir (Cunninghamia lanceolata (Lamb.) Hook.) was graded to four different classes as SS, No. 1, No.2 and No.3, according to national lumber grades authority (NLGA) for structure light framing and structure joists and planks. The properties of apparent density was determined at 15% moisture content, bending strength and stiffness were tested according to American Society for Testing and Materials (ASTM) D198-99, and dynamic modulus of elasticity (Eusw) was measured by ultrasonic technique, for predicting the flexural properties of different grade lumbers. The results showed that Eosw was larger than the static MOE. The relationship between Eusw and static MOE was significant at 0.01 level, and the determination coefficients (R2) of the four grade lumbers followed the sequence as R^2No.2 (0.616)〉 R^2ss (0.567)〉 R^2No1 (0.366)〉 R^2No.3 (0.137). The R^2 of Fusw and MOR were lower than that of the Etru and MOR for each grade. The Eusw of all the grade lumbers, except No.3-grade, had significant correlation with the static MOE and MOR, thus the bending strengthof those grade lumbers can be estimated by the E The Etru valuesof four grade lumbers followed a sequence as No.2-grade (10.701 GPa) 〉 SS-grade (10.359 GPa) 〉 No.l-grade (9.840 GPa) 〉 No.3-grade (9.554 GPa). For the same grade dimension lumber, its Eusw value was larger than static MOE. Mean values of MOR for four grade lumbers follow a sequence as No.2-grade (48.67 MPa) 〉 SS-grade (48.16 MPa) 〉 No.3-grade (46.55 MPa) 〉 No. 1-grade (43.39MPa).展开更多
The resonator fiber optic gyro (R-FOG) ,which utilizes a resonance frequency change due to the Sagnac effect,is a promising candidate for the next generation inertial rotation sensor. In this study, an open-loop R-F...The resonator fiber optic gyro (R-FOG) ,which utilizes a resonance frequency change due to the Sagnac effect,is a promising candidate for the next generation inertial rotation sensor. In this study, an open-loop R-FOG is set up using phase modulation spectroscopy. First,the demodulation curve is obtained using a lock-in amplifier. From the demodulation signal,a gyro dynamic range of ± 4.2rad/s is obtained. Then,using different phase modulation frequencies,the open-loop gyro output signal is measured when the gyro is rotated clockwise or counterclockwise. The bias drift as a function of time is also measured. The fluctuation of the output over 5s is about 0.02rad/s. The drift can be reduced by taking countermeasures against system noise.展开更多
[Objective] The aim was to explore technology on processing feces in duck farms at scale, providing guiding method for effective control of environmental issues in breeding farms. [Methed] Bio-safety disposal and reco...[Objective] The aim was to explore technology on processing feces in duck farms at scale, providing guiding method for effective control of environmental issues in breeding farms. [Methed] Bio-safety disposal and recovering processing of excrements in a duck farm were researched based on technology of configuration, detection, digestion, EM and poultry breeding. [Result] The integrated application technology is quite simple and the cost is not high. During breeding period, excellent organic fertilizers and high-protein forages could be obtained without any antibiotics. Furthermore, secondary wastes and pollution would not occur. In addition, ammonia was lower in excrements processed with earthworm and air pollution was reduced; the produced humus provided organic fertilizers and improved barren soils. [Conclusion] The research provides references of multi-technology integration for related industries.展开更多
According to the heating effect caused by interaction between matters,a series of experiments on the interaction between drugs and cells from human bodies,DNA and physiological saline have been carried out with a MS-8...According to the heating effect caused by interaction between matters,a series of experiments on the interaction between drugs and cells from human bodies,DNA and physiological saline have been carried out with a MS-80 standard Calvet microcalorimeter.The experiments include: (1) Thermokinetic studies of the effect of anticancer drugs [sodium norcantharidate (ASN),the bioac- tire materials (Sp.P and Sp.S) from algae etc.]on the cancer cells [Hela,human breast carcinoma (Bcap-37),human adenocarcinoma gastric cells (SGc-7901 and MCF-7) etc.] and the normal cells from human bodies [diploid fibroblasts from human fetal lung (2BS) etc.] at 310.15 K:(2) Studies of the in- tercalation binding of some alkaloidal drugs with the bioactivity to inhibit monoamine oxidase (harmalinc and harmine etc.) to call thymus DNA in neutral aqueous solution at 298.15 K:(3) Studies of the interaction between long acting drugs (long acting oral contraceptive-norgestrel etc.) and slow- releasing drug (Contac) and aqueous solution of 0.9% NaCI at 310.15 K.All the experimental results have given their characteristic thermograms and the interaction enthalpy changes.On the analysis of all the results,the authors put forward a method on application of microcalorimetric technique for screen- ing and examination of medicines.The principle of application and the experimental operation of this method have been expounded,and some results of the above experiments have been discussed.As one of the methods for screening and examining medicines,the microcalorimctric method has some distin- guished features and advantages over other methods.展开更多
In this paper, we present a novel Support Vector Machine active learning algorithm for effective 3D model retrieval using the concept of relevance feedback. The proposed method learns from the most informative objects...In this paper, we present a novel Support Vector Machine active learning algorithm for effective 3D model retrieval using the concept of relevance feedback. The proposed method learns from the most informative objects which are marked by the user, and then creates a boundary separating the relevant models from irrelevant ones. What it needs is only a small number of 3D models labelled by the user. It can grasp the user's semantic knowledge rapidly and accurately. Experimental results showed that the proposed algorithm significantly improves the retrieval effectiveness. Compared with four state-of-the-art query refinement schemes for 3D model retrieval, it provides superior retrieval performance after no more than two rounds of relevance feedback.展开更多
A sensitive and rapid single step real time (rt) RT-PCR was standardized using one-step Brilliant SYBR Green kit for detection and semi-quantitation ofpeste des petitis ruminants virus (PPRV) using the virus RNA a...A sensitive and rapid single step real time (rt) RT-PCR was standardized using one-step Brilliant SYBR Green kit for detection and semi-quantitation ofpeste des petitis ruminants virus (PPRV) using the virus RNA and matrix (M) protein gene-specific primers and compared with established conventional RT-PCR and TaqMan RT-PCR. The assay amplifies a 124 bp fragment of the PPRV M gene with Tm of 78.28 to 78.50. The assay was linear within a range of 50 ng to 0.5 fg total virus RNA with a detection limit (sensitivity) of 0.5 fg. Based on the serial dilution of the live-attenuated PPR vaccine virus, the detection limit was -0.0001 cell culture infectious dose 50% units (TCID50). Additionally, swab materials spiked with known titre of vaccine virus were equally well detected in the assay. The standardized rt RT-PCR was easily employed for the detection of PPRV nucleic acid directly in the field and experimental clinical samples. The assay detected the PPRV nucleic acid as early as 3 day post infection (dpi) and up to 20 dpi in swab materials from the experimental samples. The assay was rapid and more sensitive than TaqMan and conventional RT-PCR in the detection of PPRV nucleic acid from the PPR suspected clinical samples of sheep and goats. Therefore, the established, simplified SYBR green rt RT-PCR is an alternative test to the already existing various diagnostic assays and could be useful for rapid clinical diagnosis with advantage in reducing risk of contamination.展开更多
AIM: To investigate the value of contrast-enhanced C3-MODE technology in differentiating malignant nodules of liver from the benign ones. METHODS: Forty-six nodules in 36 patients (29 men and 7 women) were studied by ...AIM: To investigate the value of contrast-enhanced C3-MODE technology in differentiating malignant nodules of liver from the benign ones. METHODS: Forty-six nodules in 36 patients (29 men and 7 women) were studied by contrast-enhanced C3-MODE technology and contrast-enhanced CT in 1 wk before the biopsy or operation. A low MI monitor and a high MI flash imaging were intermittently performed. After the injection of contrast agent, the period from 10 to 30 s and the time later than 100 s were respectively defined as early arterial phase and the late phase. The vascularities of the liver nodules in the two phases were combined for differential diagnosis. Corresponding to the pathological diagnosis, the accuracy, sensitivity and specificity of contrast-enhanced C3-MODE technology were compared to those of contrast-enhanced CT. RESULTS: By C3-MODE technology, 33 of the 46 liver nodules were demonstrated as defected area in the late phase and were diagnosed as malignant tumors. Of them, 28 with hypervascularity in the early arterial phase were assessed as hepatocellular carcinoma, the other five nodules with rim-like enhancement in the early arterial phase were diagnosed as metastatic tumors. Thirteen nodules were shown as iso or hypervascularity in the late phase as well as centripetal filling in the early arterial phase and we made a diagnosis of hemangioma. Corresponding to the pathological results, the sensitivity, specificity and accuracy of contrast-enhanced C3-MODE technology in differentiating malignant and benign nodules in the liver were 97.0%, 92.3% and 95.7%, respectively. With comparison to those of contrast CT (sensitivity, 94.1%; specificity, 91.7%; accuracy, 93.5%), the difference was not significant. CONCLUSION: Contrast-enhanced C3-MODE technology can effectively differentiate malignant liver tumors from the benign nodules. It highly agrees diagnostically with the pathology. We suggest that it provides a new approach for differential diagnosis of liver nodules in addition to contrast-enhanced CT.展开更多
It is well known that angiogenesis is critical in the transition from premalignant to malignant lesions.Consequently,early detection and diagnosis based on morphological changes to the microvessels are crucial.In the ...It is well known that angiogenesis is critical in the transition from premalignant to malignant lesions.Consequently,early detection and diagnosis based on morphological changes to the microvessels are crucial.In the last few years,new imaging techniques which utilize the properties of light-tissue interaction have been developed to increase early diagnosis of gastrointestinal(GI) tract neoplasia.We analyzed several "red-flag" endoscopic techniques used to enhance visualization of the vascular pattern of preneoplastic and neoplastic lesions(e.g.trimodal imaging including autofluorescence imaging,magnifying endoscopy and narrow band imaging).These new endoscopic techniques provide better visualization of mucosal microsurface structure and microvascular architecture and may enhance the diagnosis and characterization of mucosal lesions in the GI tract.In the near future,it is expected that trimodal imaging endoscopy will be practiced as a standard endoscopy technique as it is quick,safe and accurate for making a precise diagnosis of gastrointestinal pathology,with an emphasis on the diagnosis of early GI tract cancers.Further large-scale randomized controlled trials comparing these modalities in different patient subpopulations are warranted before their endorsement in the routine practice of GI endoscopy.展开更多
A new non-contact inspection technique based on laser-PSD (position sensitive detector) to inspect the inner surface of small-diameter pipe is proposed,and the corresponding sensor has been developed. After being re...A new non-contact inspection technique based on laser-PSD (position sensitive detector) to inspect the inner surface of small-diameter pipe is proposed,and the corresponding sensor has been developed. After being reflected by two mirrors in series,a laser beam is projected onto the inner wall of a pipe as a small light spot and is read by a two-dimensional PSD. Based on the signals from the PSD and the structure parameters of the sensor,the spot position on the wall can be calculated in a local 3D coordinate system. The spot controlled by the micro-motor driven mirrors will scan a closed section ring on the inner wall of the pipe to obtain the relative coordinates of all the sampled points. The data will be then processed through data segmentation and least square fitting,to reconstruct the section curve used for obtaining the radius and the defect description of the section. Driven by a micro-pipe robot,the sensor can inspect a long curved pipe and obtain its 3-D reconstruction. An inspection system based on this technique can detect the mini-diarneter pipe with an inner diameter of 9.5 mm-10.5 mm and a curvature radius larger than 100 mm at a measurement accuracy of the inner surface defect of ±0.1 mm.展开更多
With the rapid development of wireless technologies, it is possible for Chinese greenhouses to be equipped with wireless sensor networks due to their low-cost, simplicity and mobility. In the current study, we compare...With the rapid development of wireless technologies, it is possible for Chinese greenhouses to be equipped with wireless sensor networks due to their low-cost, simplicity and mobility. In the current study, we compared the advantages of ZigBee with other two similar wireless networking protocols, Wi-Fi and Bluetooth, and proposed a wireless solution for green- house monitoring and control system based on ZigBee technology. As an explorative application of ZigBee technology in Chinese greenhouse, it may promote Chinese protected agriculture.展开更多
Foreign bodies in the colon are encountered with increasing frequency, but only sporadic reports concerning their management have appeared in the literature. While most ingested foreign bodies usually pass through the...Foreign bodies in the colon are encountered with increasing frequency, but only sporadic reports concerning their management have appeared in the literature. While most ingested foreign bodies usually pass through the gastrointestinal tract uneventfully, sharp foreign bodies such as toothpicks infrequently cause intestinal perforation and may even result in death. We report our experience with a patient with a sigmoid colon pseudodiverticulum formation, a complication of accidental ingestion of a toothpick that was diagnosed and successfully managed colonoscopically.展开更多
文摘This study aimed to achieve rapid detection of Pseudomonas syringae pv.tabaci,the pathogen of tobacco wildfire disease.The specific primers and probes for recombinase-aided amplification(RAA)were designed with HrpZ as the target gene.RAA was then combined with the lateral flow dipstick(LFD)to establish a LFD-RAA-based rapid detection system for the pathogen.Furthermore,the detection performance of the established method was tested.The results showed that the LFDRAA method had high specificity.The amplification could be completed after 25 min of reaction at 39℃.The sensitivity of the established method reached 0.0001 ng/μL,which was superior to that of PCR detection.Moreover,the LFD-RAA method could quickly detect P.syringae pv.tabaci from tobacco leaves,demonstrating field applicability.To sum up,the LFD-RAA method established in this study can be applied in the rapid detection and early diagnosis of tobacco wildfire disease.
文摘Objective To observe the cervical elasticity of healthy adult nulliparous women at different age groups and different stages of menstrual cycle with E-Cervix imaging technology.Methods A total of 218 healthy adult nulliparous women who underwent transvaginal ultrasound examination for routine physical examination were retrospectively enrolled,including 103 in follicular phase,78 in ovulation phase and 37 in luteal phase.Cervical canal length(CL)and E-Cervix elasticity parameters were compared among different age groups and different stages of menstrual cycle,including elasticity contrast index(ECI),hardness ratio(HR),cervical internal and external orifice strain values(IOS and EOS)and IOS/EOS ratio.Results No significant difference of CL nor cervical elasticity parameters was detected among healthy adult nulliparous women at different age groups(all P>0.05).There were significant differences of ECI,HR and IOS among different menstrual cycle stages(all P<0.05),among which women in follicular phase had higher ECI and IOS but lower HR than those in luteal phase(all P<0.05).Conclusion No significant difference of cervical elasticity existed among healthy adult nulliparous women at different age groups.Meanwhile,cervical elasticity of healthy adult nulliparous women changed during menstrual cycle,in follicular phase had higher ECI and IOS but lower HR than in luteal phase.
文摘Loop-mediated isothermal amplification(LAMP) is an amplification method developed by Notomi et al and has been applied successfully for the detection of many viruses.This paper introduced the current status of LAMP and recent developments,and the method applying in the diagnosis of animal viruses in abroad.
基金This project was supported by a grant from the Natural Science Foundation of Hubei Province (No. 2002AB130)
文摘Abstract Objective: To evaluate the diagnostic value of two-phase multidetector-row spiral CT threedimensional reconstruction technique in TNM staging of gastric cancer. Methods: In 29 patients with gastric carcinoma pathologically conformed, plan scans were done firstly. Two-phase spiral CT was performed within one breathhold each. Distension of the stomach was achieved by intravenous application of anisodamine and effervescent granules. After bolus injection of contrast medium, scanning was performed in the arterial and venous phase, and the source images were thin reconstructed. The stomach to three-dimension analysis was constructed by volume rendering (VR) multiplanaz volume reconstruction (MPVR), shaded surface display (SSD) and CT virtual gastroscopy (CTVG) technique. In combination with the sources images, gastric tumour invasion and lymph node metastasis was assessed, and TNM staging was performed. Results: In 29 cases of gastric carcinoma, the sensitivity and specificity of two-phase multidetector-row spiral CT three-dimensional reconstruction technique in T1, T2, T3 and Ta staging, the sensitivity and specificity was 50% and 50%, 87.5% and 77.8%, 83.3% and 76.9% and 100% and 80% respectively. For the N staging, the sensitivity and specificity in No, N1, and N2 N3 was 83.3% and 71.4%, 87.5% and 77.8% and 81.8% and 75% respectively. The sensitivity and the specificity for M1 staging was 100%. Conclusion: The reconstruction technique in combination with 16-slices spiral-CT can perform TNM staging well and effectively guide the choice of the surgical procedures for gastric cancer.
基金Standard system on forestry engineering of Ministry ofScience and Technology ( 2004DEA70900-1).
文摘The dimension lumber (45mm×90mm×3700mm) of plantation Chinese fir (Cunninghamia lanceolata (Lamb.) Hook.) was graded to four different classes as SS, No. 1, No.2 and No.3, according to national lumber grades authority (NLGA) for structure light framing and structure joists and planks. The properties of apparent density was determined at 15% moisture content, bending strength and stiffness were tested according to American Society for Testing and Materials (ASTM) D198-99, and dynamic modulus of elasticity (Eusw) was measured by ultrasonic technique, for predicting the flexural properties of different grade lumbers. The results showed that Eosw was larger than the static MOE. The relationship between Eusw and static MOE was significant at 0.01 level, and the determination coefficients (R2) of the four grade lumbers followed the sequence as R^2No.2 (0.616)〉 R^2ss (0.567)〉 R^2No1 (0.366)〉 R^2No.3 (0.137). The R^2 of Fusw and MOR were lower than that of the Etru and MOR for each grade. The Eusw of all the grade lumbers, except No.3-grade, had significant correlation with the static MOE and MOR, thus the bending strengthof those grade lumbers can be estimated by the E The Etru valuesof four grade lumbers followed a sequence as No.2-grade (10.701 GPa) 〉 SS-grade (10.359 GPa) 〉 No.l-grade (9.840 GPa) 〉 No.3-grade (9.554 GPa). For the same grade dimension lumber, its Eusw value was larger than static MOE. Mean values of MOR for four grade lumbers follow a sequence as No.2-grade (48.67 MPa) 〉 SS-grade (48.16 MPa) 〉 No.3-grade (46.55 MPa) 〉 No. 1-grade (43.39MPa).
文摘The resonator fiber optic gyro (R-FOG) ,which utilizes a resonance frequency change due to the Sagnac effect,is a promising candidate for the next generation inertial rotation sensor. In this study, an open-loop R-FOG is set up using phase modulation spectroscopy. First,the demodulation curve is obtained using a lock-in amplifier. From the demodulation signal,a gyro dynamic range of ± 4.2rad/s is obtained. Then,using different phase modulation frequencies,the open-loop gyro output signal is measured when the gyro is rotated clockwise or counterclockwise. The bias drift as a function of time is also measured. The fluctuation of the output over 5s is about 0.02rad/s. The drift can be reduced by taking countermeasures against system noise.
基金Supported by Program from Education Department of Sichuan Province(112B115)~~
文摘[Objective] The aim was to explore technology on processing feces in duck farms at scale, providing guiding method for effective control of environmental issues in breeding farms. [Methed] Bio-safety disposal and recovering processing of excrements in a duck farm were researched based on technology of configuration, detection, digestion, EM and poultry breeding. [Result] The integrated application technology is quite simple and the cost is not high. During breeding period, excellent organic fertilizers and high-protein forages could be obtained without any antibiotics. Furthermore, secondary wastes and pollution would not occur. In addition, ammonia was lower in excrements processed with earthworm and air pollution was reduced; the produced humus provided organic fertilizers and improved barren soils. [Conclusion] The research provides references of multi-technology integration for related industries.
文摘According to the heating effect caused by interaction between matters,a series of experiments on the interaction between drugs and cells from human bodies,DNA and physiological saline have been carried out with a MS-80 standard Calvet microcalorimeter.The experiments include: (1) Thermokinetic studies of the effect of anticancer drugs [sodium norcantharidate (ASN),the bioac- tire materials (Sp.P and Sp.S) from algae etc.]on the cancer cells [Hela,human breast carcinoma (Bcap-37),human adenocarcinoma gastric cells (SGc-7901 and MCF-7) etc.] and the normal cells from human bodies [diploid fibroblasts from human fetal lung (2BS) etc.] at 310.15 K:(2) Studies of the in- tercalation binding of some alkaloidal drugs with the bioactivity to inhibit monoamine oxidase (harmalinc and harmine etc.) to call thymus DNA in neutral aqueous solution at 298.15 K:(3) Studies of the interaction between long acting drugs (long acting oral contraceptive-norgestrel etc.) and slow- releasing drug (Contac) and aqueous solution of 0.9% NaCI at 310.15 K.All the experimental results have given their characteristic thermograms and the interaction enthalpy changes.On the analysis of all the results,the authors put forward a method on application of microcalorimetric technique for screen- ing and examination of medicines.The principle of application and the experimental operation of this method have been expounded,and some results of the above experiments have been discussed.As one of the methods for screening and examining medicines,the microcalorimctric method has some distin- guished features and advantages over other methods.
基金the National Basic Research Program (973) of China (No. 2004CB719401)the National Research Foundation for the Doctoral Program of Higher Education of China (No.20060003060)
文摘In this paper, we present a novel Support Vector Machine active learning algorithm for effective 3D model retrieval using the concept of relevance feedback. The proposed method learns from the most informative objects which are marked by the user, and then creates a boundary separating the relevant models from irrelevant ones. What it needs is only a small number of 3D models labelled by the user. It can grasp the user's semantic knowledge rapidly and accurately. Experimental results showed that the proposed algorithm significantly improves the retrieval effectiveness. Compared with four state-of-the-art query refinement schemes for 3D model retrieval, it provides superior retrieval performance after no more than two rounds of relevance feedback.
基金Indian Council of Agricultural Research,New Delhi,India under Niche Area of Excellence:Production and Quality control of Veterinary Immunodiganostics and immunoprophylactics(F.No.10(11)2005-EP&D.dated 15.12.2005)
文摘A sensitive and rapid single step real time (rt) RT-PCR was standardized using one-step Brilliant SYBR Green kit for detection and semi-quantitation ofpeste des petitis ruminants virus (PPRV) using the virus RNA and matrix (M) protein gene-specific primers and compared with established conventional RT-PCR and TaqMan RT-PCR. The assay amplifies a 124 bp fragment of the PPRV M gene with Tm of 78.28 to 78.50. The assay was linear within a range of 50 ng to 0.5 fg total virus RNA with a detection limit (sensitivity) of 0.5 fg. Based on the serial dilution of the live-attenuated PPR vaccine virus, the detection limit was -0.0001 cell culture infectious dose 50% units (TCID50). Additionally, swab materials spiked with known titre of vaccine virus were equally well detected in the assay. The standardized rt RT-PCR was easily employed for the detection of PPRV nucleic acid directly in the field and experimental clinical samples. The assay detected the PPRV nucleic acid as early as 3 day post infection (dpi) and up to 20 dpi in swab materials from the experimental samples. The assay was rapid and more sensitive than TaqMan and conventional RT-PCR in the detection of PPRV nucleic acid from the PPR suspected clinical samples of sheep and goats. Therefore, the established, simplified SYBR green rt RT-PCR is an alternative test to the already existing various diagnostic assays and could be useful for rapid clinical diagnosis with advantage in reducing risk of contamination.
基金Supported by the Science Foundation of the Department of Science and Technology of Guangdong Province, No. 2002C30315
文摘AIM: To investigate the value of contrast-enhanced C3-MODE technology in differentiating malignant nodules of liver from the benign ones. METHODS: Forty-six nodules in 36 patients (29 men and 7 women) were studied by contrast-enhanced C3-MODE technology and contrast-enhanced CT in 1 wk before the biopsy or operation. A low MI monitor and a high MI flash imaging were intermittently performed. After the injection of contrast agent, the period from 10 to 30 s and the time later than 100 s were respectively defined as early arterial phase and the late phase. The vascularities of the liver nodules in the two phases were combined for differential diagnosis. Corresponding to the pathological diagnosis, the accuracy, sensitivity and specificity of contrast-enhanced C3-MODE technology were compared to those of contrast-enhanced CT. RESULTS: By C3-MODE technology, 33 of the 46 liver nodules were demonstrated as defected area in the late phase and were diagnosed as malignant tumors. Of them, 28 with hypervascularity in the early arterial phase were assessed as hepatocellular carcinoma, the other five nodules with rim-like enhancement in the early arterial phase were diagnosed as metastatic tumors. Thirteen nodules were shown as iso or hypervascularity in the late phase as well as centripetal filling in the early arterial phase and we made a diagnosis of hemangioma. Corresponding to the pathological results, the sensitivity, specificity and accuracy of contrast-enhanced C3-MODE technology in differentiating malignant and benign nodules in the liver were 97.0%, 92.3% and 95.7%, respectively. With comparison to those of contrast CT (sensitivity, 94.1%; specificity, 91.7%; accuracy, 93.5%), the difference was not significant. CONCLUSION: Contrast-enhanced C3-MODE technology can effectively differentiate malignant liver tumors from the benign nodules. It highly agrees diagnostically with the pathology. We suggest that it provides a new approach for differential diagnosis of liver nodules in addition to contrast-enhanced CT.
文摘It is well known that angiogenesis is critical in the transition from premalignant to malignant lesions.Consequently,early detection and diagnosis based on morphological changes to the microvessels are crucial.In the last few years,new imaging techniques which utilize the properties of light-tissue interaction have been developed to increase early diagnosis of gastrointestinal(GI) tract neoplasia.We analyzed several "red-flag" endoscopic techniques used to enhance visualization of the vascular pattern of preneoplastic and neoplastic lesions(e.g.trimodal imaging including autofluorescence imaging,magnifying endoscopy and narrow band imaging).These new endoscopic techniques provide better visualization of mucosal microsurface structure and microvascular architecture and may enhance the diagnosis and characterization of mucosal lesions in the GI tract.In the near future,it is expected that trimodal imaging endoscopy will be practiced as a standard endoscopy technique as it is quick,safe and accurate for making a precise diagnosis of gastrointestinal pathology,with an emphasis on the diagnosis of early GI tract cancers.Further large-scale randomized controlled trials comparing these modalities in different patient subpopulations are warranted before their endorsement in the routine practice of GI endoscopy.
文摘A new non-contact inspection technique based on laser-PSD (position sensitive detector) to inspect the inner surface of small-diameter pipe is proposed,and the corresponding sensor has been developed. After being reflected by two mirrors in series,a laser beam is projected onto the inner wall of a pipe as a small light spot and is read by a two-dimensional PSD. Based on the signals from the PSD and the structure parameters of the sensor,the spot position on the wall can be calculated in a local 3D coordinate system. The spot controlled by the micro-motor driven mirrors will scan a closed section ring on the inner wall of the pipe to obtain the relative coordinates of all the sampled points. The data will be then processed through data segmentation and least square fitting,to reconstruct the section curve used for obtaining the radius and the defect description of the section. Driven by a micro-pipe robot,the sensor can inspect a long curved pipe and obtain its 3-D reconstruction. An inspection system based on this technique can detect the mini-diarneter pipe with an inner diameter of 9.5 mm-10.5 mm and a curvature radius larger than 100 mm at a measurement accuracy of the inner surface defect of ±0.1 mm.
基金Project (No. 2005C22060) supported by the Science and Technology Department of Zhejiang Province, China
文摘With the rapid development of wireless technologies, it is possible for Chinese greenhouses to be equipped with wireless sensor networks due to their low-cost, simplicity and mobility. In the current study, we compared the advantages of ZigBee with other two similar wireless networking protocols, Wi-Fi and Bluetooth, and proposed a wireless solution for green- house monitoring and control system based on ZigBee technology. As an explorative application of ZigBee technology in Chinese greenhouse, it may promote Chinese protected agriculture.
文摘Foreign bodies in the colon are encountered with increasing frequency, but only sporadic reports concerning their management have appeared in the literature. While most ingested foreign bodies usually pass through the gastrointestinal tract uneventfully, sharp foreign bodies such as toothpicks infrequently cause intestinal perforation and may even result in death. We report our experience with a patient with a sigmoid colon pseudodiverticulum formation, a complication of accidental ingestion of a toothpick that was diagnosed and successfully managed colonoscopically.