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鸭各级卵泡中抑制素α和抑制素/活化素β_A亚基信使RNA表达丰度的研究 被引量:5
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作者 傅衍 牛冬 +4 位作者 阮晖 余旭平 陈功 何国庆 杨培新 《Acta Genetica Sinica》 SCIE CAS CSCD 北大核心 2001年第6期502-509,共8页
采用非常灵敏的定量竞争 RT-PCR技术对鸭各级卵泡中抑制素α和βA亚基的mRNA表达丰度作了研究,发现在各级卵泡中均有此两亚基mRNA的表达,大卵泡中的α亚基表达量要高于βA亚基。α亚基mRNA在小黄卵泡(SYF)... 采用非常灵敏的定量竞争 RT-PCR技术对鸭各级卵泡中抑制素α和βA亚基的mRNA表达丰度作了研究,发现在各级卵泡中均有此两亚基mRNA的表达,大卵泡中的α亚基表达量要高于βA亚基。α亚基mRNA在小黄卵泡(SYF)中表达量最高,以其mRNA的平均相对丰度为1.00,则F1、F2、F3、F4/5、LWF(大白卵泡)中α亚基mRNA的平均相对含量分别为: 0.26±0.05、0.28±0.07、0.57±0.12、0.98±0.09、0.026±0.006。βA亚基 mRNA在F1中表达量最高,以其mRNA的平均相对丰度为1.00,则 F2、F3、F4/5、SYE、LWF中βA亚基mRNA的平均相对含量分别为:0.218±0.09、0.111±0.03、0.058±0.011、0.053±0.013、0.005±0.002。结果显示,随着卵泡的增大α亚基表达量降低, βA亚基却增加,说明在卵泡发育过程中, α和βA亚基的表达是独立调节的,另外,βA亚基在F1中的大量表达,说明F1可能是形成二聚体抑制素/活化素的主要场所。 展开更多
关键词 定量竞争RT-PCR 抑制α亚基 抑制素/活化素βA亚基 卵泡 MRNA 表达丰度
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东北白鹅抑制素/活化素β_B亚基成熟区cDNA的克隆 被引量:3
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作者 赵悦 杨焕民 +2 位作者 王琳琳 钱峰 孙永强 《黑龙江八一农垦大学学报》 2007年第2期59-62,共4页
利用RT-PCR技术从东北白鹅卵泡的总RNA中扩增出了抑制素βB亚基成熟区序列,经RCR扩增出357bp片段,该片段与pMD18-T载体连接,转化到JM109感受态细胞,所得阳性克隆进行双酶切鉴定和PCR鉴定,并进行了测序分析,获得的克隆序列与设计的序列... 利用RT-PCR技术从东北白鹅卵泡的总RNA中扩增出了抑制素βB亚基成熟区序列,经RCR扩增出357bp片段,该片段与pMD18-T载体连接,转化到JM109感受态细胞,所得阳性克隆进行双酶切鉴定和PCR鉴定,并进行了测序分析,获得的克隆序列与设计的序列一致。 展开更多
关键词 东北白鹅 抑制素/活化素 βB亚基cDNA 克隆
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仙居鸡抑制素/活化素β_B亚基成熟区cDNA的克隆及序列分析
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作者 牛冬 阮晖 +4 位作者 傅衍 余旭平 陈功 何国庆 杨培新 《中国兽医学报》 CAS CSCD 北大核心 2002年第5期433-435,共3页
根据发表的哺乳类抑制素 /活化素 βB亚基 c DNA序列设计引物 ,运用 RT- PCR技术从仙居鸡卵泡的颗粒细胞总RNA中扩增出抑制素 /活化素βB亚基成熟区序列 ,并进行了克隆和测序。结果显示 ,鸡成熟βB亚基是由 115个氨基酸(aa)残基组成的... 根据发表的哺乳类抑制素 /活化素 βB亚基 c DNA序列设计引物 ,运用 RT- PCR技术从仙居鸡卵泡的颗粒细胞总RNA中扩增出抑制素 /活化素βB亚基成熟区序列 ,并进行了克隆和测序。结果显示 ,鸡成熟βB亚基是由 115个氨基酸(aa)残基组成的蛋白质 ,具有 9个半胱氨酸残基 ,与发表的哺乳类相应序列对比 ,其核苷酸序列的同源性为 79.7%~82 .9%,其编码氨基酸序列的同源性为 95 .7%~ 98.3%。 βB亚基成熟区半胱氨酸残基的数目和位置与发表的哺乳类相同。说明该亚基的序列及结构在不同物种间具高度保守性 ,提示抑制素 /活化素 βB亚基可能具重要的生理功能。 展开更多
关键词 成熟区 抑制素/活化素βB亚基 CDNA克隆 序列分析
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Effect of Lime Nitrogen on the Transformation of Ureain Soils 被引量:2
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作者 HE NIAN-ZU YU SHEN +1 位作者 YE ZHI-QIAN OUYANG HANG and XIONG JIN-SHAN(Depariment of Land Science and APPlied Chemistry, Zhejtang Agricultural University, Hangzhou 310029( China)(Institrte of Soil Science, Academia Sintica, Nanjing 210008 (China)(Juhua Gr 《Pedosphere》 SCIE CAS CSCD 1995年第3期221-227,共7页
Laboratory incubation experiment was conducted to study the effect of lime nitrogen(LN) on transfor-mation of iirea-N in three paddy soils. The results showed that LN had an inhibitory effect on urease activityin thes... Laboratory incubation experiment was conducted to study the effect of lime nitrogen(LN) on transfor-mation of iirea-N in three paddy soils. The results showed that LN had an inhibitory effect on urease activityin these soils especially in the first 5 days, and that in the first 20 days of incubation, the amount of NH-Nderived from urea was lower in the soil with LN than in the soil without LN. While after 30 days the ainountof NH-N was higher in the mature haplic paddy soil developed on Quaternary red clay(MHPS) with LNthan that in the soil without LN. The amonnt of NH_3-N volatilized was decreased in the earlier stage andincreased in the later stage of incubation in the MHPS by the addition of LN. 展开更多
关键词 lime nitrogen nitrification inhibitor ureai urease activityi urease inhibitor
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RELATIONSHIP BETWEEN SOMATOSTATIN RECEPTORS AND ACTIVATION OF HEPATIC STELLATE CELL 被引量:2
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作者 潘勤 李定国 +3 位作者 陆汉明 尤汉宁 徐芹芳 陆良勇 《Journal of Shanghai Second Medical University(Foreign Language Edition)》 2004年第2期83-83,共1页
Objective To investigate the relationship between expression of somatostatin receptors(SSTRs) and activation of rat hepatic stellate cell (HSC). Methods HSCs were isolated from rats by in situ perfusion and single-ste... Objective To investigate the relationship between expression of somatostatin receptors(SSTRs) and activation of rat hepatic stellate cell (HSC). Methods HSCs were isolated from rats by in situ perfusion and single-step density gradient centrifugation, and then SSTR1-5 mRNA levels in the differentiated first passage HSCs were detected by means of reverse transcription polymerase chain reaction. On the other hand, hepatic fibrosis was induced in adult male Sprague-Dawley rats by carbon tetrachloride intoxication, and the expression of SSTR1-5 in normal as well as fibrotic liver was measured by immunohistochemical staining. Results SSTR mR-NA and SSTR could not be found in freshly isolated rat HSCs and normal rat liver. But SSTR1-3 mRNA appeared as HSCs became wholly activated, and SSTR1-3 could also be identified on the membrane of activated HSCs in the peri-sinusoid space, fibrous septa, etc. Conclusion The expression of SSTR1-3 in the rat HSC is closely related to its activation. This may reflect one of the main negative regulation mechanisms in the course of HSC activation. 展开更多
关键词 somatostatin receptor hepatic stellate cell activation
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