We investigated the effects of dried macroalga Gracilaria lemaneiform (Rhodophyta) on photosynthesis of the bloom-forming microalga Chaetoceros curvisetus. C. curvisetus was cultured with different amounts of dried ...We investigated the effects of dried macroalga Gracilaria lemaneiform (Rhodophyta) on photosynthesis of the bloom-forming microalga Chaetoceros curvisetus. C. curvisetus was cultured with different amounts of dried G. lemaneiformis under controlled laboratory conditions. We measured the photosynthetic oxygen evolution rate and established the chlorophyll a fluorescence transient (OJIP) curve coupled with its specific parameters. We observed concentration-dependent and time-dependent relationships between dried G. lemaneiformis and inhibition of photosynthesis in C. curvisetus. Co-culture with dried G. lemaneiformis also resulted in a decrease in the light-saturated maximum photosynthetic oxygen evolution rate (P^ax) in C. curvisetus, and a decrease in the OJIP curve along with its specific parameters; the maximum photochemical efficiency of PSII (FJFm), the amount of active PSII reaction centers per excited cross section at t=0 and t=--tFM (RC/CS0 and RC/CSm, respectively), the absorption flux per excited cross section at t=0 (ABS/ CS0), and the efficiency with which a trapped exciton moves an electron into the electron transport chain (~u0). The dark respiration rate (Rd) increased in C. curvisetus co-cultured with dried G. lemaneiformis. The JIP-test and the oxygen evolution results indicated that dried G. lemaneiJbrmis decreased the number of active reaction centers, blocked the electron transport chain, and damaged the oxygen-evolving complex of C. curvisetus. This result indicated that dried fragments of G. lemaneiformis could effectively inhibit photosynthesis of C. curvisetus, and thus, could serve as a functional product to control and mitigate C. curvisetus blooms.展开更多
Growth inhibition effect of different concentration of distilled water extract and four polar organic solvent (methanol, acetone, ether and chloroform) extracts of Ulva pertusa on three typical red tide microalgae (He...Growth inhibition effect of different concentration of distilled water extract and four polar organic solvent (methanol, acetone, ether and chloroform) extracts of Ulva pertusa on three typical red tide microalgae (Heterosigma akashiwo, Alexandrium tamarense and Prorocentrum micans) were inves- tigated. Liquid-liquid fractionation and HPLC analysis for methanol extract of U. pertusa were carried out. Growth of the three microalgae was significantly inhibited by the distilled water extract of U. pertusa at relatively higher concentration. However, the cells of the three microalgae did not die completely even at high concentration. Methanol extract of U. pertusa showed the highest growth inhibition on the three mi- croalgae, and all the cells of the three microalgae were killed at relatively high concentration. The other three organic solvent extracts of U. pertusa had no apparent effect on the three microalgae. The results of bioassays and HPLC analysis suggested that the inhibitory substances in U. pertusa to the microalgal growth had relatively high polarities. H. akashiwo was the most sensitive one while A. tamarense was the most tolerant one to the growth inhibitory substances.展开更多
基金Supported by the National Key Technology R&D Program(No.2012BAC07B05)the National Natural Science Foundation of China(No.31000240)the Project on the Integration of Industry,Education and Research of Guangdong Province(No.2012B091100341)
文摘We investigated the effects of dried macroalga Gracilaria lemaneiform (Rhodophyta) on photosynthesis of the bloom-forming microalga Chaetoceros curvisetus. C. curvisetus was cultured with different amounts of dried G. lemaneiformis under controlled laboratory conditions. We measured the photosynthetic oxygen evolution rate and established the chlorophyll a fluorescence transient (OJIP) curve coupled with its specific parameters. We observed concentration-dependent and time-dependent relationships between dried G. lemaneiformis and inhibition of photosynthesis in C. curvisetus. Co-culture with dried G. lemaneiformis also resulted in a decrease in the light-saturated maximum photosynthetic oxygen evolution rate (P^ax) in C. curvisetus, and a decrease in the OJIP curve along with its specific parameters; the maximum photochemical efficiency of PSII (FJFm), the amount of active PSII reaction centers per excited cross section at t=0 and t=--tFM (RC/CS0 and RC/CSm, respectively), the absorption flux per excited cross section at t=0 (ABS/ CS0), and the efficiency with which a trapped exciton moves an electron into the electron transport chain (~u0). The dark respiration rate (Rd) increased in C. curvisetus co-cultured with dried G. lemaneiformis. The JIP-test and the oxygen evolution results indicated that dried G. lemaneiJbrmis decreased the number of active reaction centers, blocked the electron transport chain, and damaged the oxygen-evolving complex of C. curvisetus. This result indicated that dried fragments of G. lemaneiformis could effectively inhibit photosynthesis of C. curvisetus, and thus, could serve as a functional product to control and mitigate C. curvisetus blooms.
基金This research supported by the Open Research Fund Program of Key Laboratory of Marine Drugs (Ocean University of China), the Ministry of Education of China also by NSFC for Talented Youths (No. 397250239) and the Project under Major State Basic Research of China (No. G1999012011)
文摘Growth inhibition effect of different concentration of distilled water extract and four polar organic solvent (methanol, acetone, ether and chloroform) extracts of Ulva pertusa on three typical red tide microalgae (Heterosigma akashiwo, Alexandrium tamarense and Prorocentrum micans) were inves- tigated. Liquid-liquid fractionation and HPLC analysis for methanol extract of U. pertusa were carried out. Growth of the three microalgae was significantly inhibited by the distilled water extract of U. pertusa at relatively higher concentration. However, the cells of the three microalgae did not die completely even at high concentration. Methanol extract of U. pertusa showed the highest growth inhibition on the three mi- croalgae, and all the cells of the three microalgae were killed at relatively high concentration. The other three organic solvent extracts of U. pertusa had no apparent effect on the three microalgae. The results of bioassays and HPLC analysis suggested that the inhibitory substances in U. pertusa to the microalgal growth had relatively high polarities. H. akashiwo was the most sensitive one while A. tamarense was the most tolerant one to the growth inhibitory substances.