目的探讨抗乙酰胆碱受体单克隆抗体A7(抗-AChR mAb A7)对实验性自身免疫性重症肌无力(EAMG)的作用. 方法经Lewis大鼠腹腔注射5 mL 20倍浓缩的含抗-AChR mAb A7培养上清液,建立EAMG被动转输模型.对照组经腹腔注射同体积的PBS.每8 h监测...目的探讨抗乙酰胆碱受体单克隆抗体A7(抗-AChR mAb A7)对实验性自身免疫性重症肌无力(EAMG)的作用. 方法经Lewis大鼠腹腔注射5 mL 20倍浓缩的含抗-AChR mAb A7培养上清液,建立EAMG被动转输模型.对照组经腹腔注射同体积的PBS.每8 h监测体重和临床症状评分.48 h后处死实验动物.采用放射免疫法检测AChR,并计算出AChR损失率.结果抗-AChR mAb A7诱导的EAMG模型AChR损失率高达24.3%～45.2%,而且AChR损失率与临床症状评分相关(r=0.74, P<0.01).抗-AChR mAb A7攻击位点在终板的AChR上.结论抗-AChR mAb A7可用于诱导EAMG模型及有关研究.展开更多
Background: Antibodies (Ab) against the acetylcholine receptor (AChR) are found in 80-90% of generalized myasthenia gravis (MG),but only in 50% of pure ocular MG. Furthermore,Ab against the muscle-specific receptor ty...Background: Antibodies (Ab) against the acetylcholine receptor (AChR) are found in 80-90% of generalized myasthenia gravis (MG),but only in 50% of pure ocular MG. Furthermore,Ab against the muscle-specific receptor tyrosine kinase (MuSK) were detected in 38-54% of patients with AChR-Ab negative (“ seronegative” ) MG,but not in pure ocular MG. Methods: 2 case reports have been analysed: Two patients (42 years old and 61 years old) with primary ocular MG were studied. Both patients complained of fluctuating bilateral ptosis and double vision lasting for 2 to 3 months. Results: AChR-Ab were negative but MuSK-Ab were clearly positive. In spite of treatment with pyridistigmin and prednisolone,the symptoms in patient 1 generalized within 4 weeks causing dysphagia,limb muscle weakness and respiratory crisis. Symptoms immediately responded to plasmapheresis. Patient 2 clearly improved under treatment with pyridostigmine,methylprednisone,and azathioprine. There was no generalizationwithin a course of 12 months. Conclusions: Both cases clearly indicate that MuSKAb can be found in primary isolated ocular MG lasting for 2 to 3 months. In a primary ocular manifestation of MG the detection of MuSK might indicate the risk for subsequent generalization and might have implications for immunosuppressive therapy. Therefore,seronegative MG requires testing for MuSK-Ab.展开更多
文摘目的探讨抗乙酰胆碱受体单克隆抗体A7(抗-AChR mAb A7)对实验性自身免疫性重症肌无力(EAMG)的作用. 方法经Lewis大鼠腹腔注射5 mL 20倍浓缩的含抗-AChR mAb A7培养上清液,建立EAMG被动转输模型.对照组经腹腔注射同体积的PBS.每8 h监测体重和临床症状评分.48 h后处死实验动物.采用放射免疫法检测AChR,并计算出AChR损失率.结果抗-AChR mAb A7诱导的EAMG模型AChR损失率高达24.3%～45.2%,而且AChR损失率与临床症状评分相关(r=0.74, P<0.01).抗-AChR mAb A7攻击位点在终板的AChR上.结论抗-AChR mAb A7可用于诱导EAMG模型及有关研究.
文摘Background: Antibodies (Ab) against the acetylcholine receptor (AChR) are found in 80-90% of generalized myasthenia gravis (MG),but only in 50% of pure ocular MG. Furthermore,Ab against the muscle-specific receptor tyrosine kinase (MuSK) were detected in 38-54% of patients with AChR-Ab negative (“ seronegative” ) MG,but not in pure ocular MG. Methods: 2 case reports have been analysed: Two patients (42 years old and 61 years old) with primary ocular MG were studied. Both patients complained of fluctuating bilateral ptosis and double vision lasting for 2 to 3 months. Results: AChR-Ab were negative but MuSK-Ab were clearly positive. In spite of treatment with pyridistigmin and prednisolone,the symptoms in patient 1 generalized within 4 weeks causing dysphagia,limb muscle weakness and respiratory crisis. Symptoms immediately responded to plasmapheresis. Patient 2 clearly improved under treatment with pyridostigmine,methylprednisone,and azathioprine. There was no generalizationwithin a course of 12 months. Conclusions: Both cases clearly indicate that MuSKAb can be found in primary isolated ocular MG lasting for 2 to 3 months. In a primary ocular manifestation of MG the detection of MuSK might indicate the risk for subsequent generalization and might have implications for immunosuppressive therapy. Therefore,seronegative MG requires testing for MuSK-Ab.
