Laser shock peening(LSP)was used to enhance the high-temperature oxidation resistance of laser melting deposited Ti45Al8Nb alloy.The microstructure and high-temperature oxidation behavior of the as-deposited Ti45Al8Nb...Laser shock peening(LSP)was used to enhance the high-temperature oxidation resistance of laser melting deposited Ti45Al8Nb alloy.The microstructure and high-temperature oxidation behavior of the as-deposited Ti45Al8Nb alloy before and after LSP were investigated by scanning electron microscopy,X-ray diffraction,and electron backscatter diffraction.The results indicated that the rate of mass gain in the as-deposited sample after LSP exhibited a decrease when exposed to an oxidation temperature of 900℃,implying that LSP-treated samples exhibited superior oxidation resistance at high temperatures.A gradient structure with a fine-grain layer,a deformed-grain layer,and a coarse-grain layer was formed in the LSP-treated sample,which facilitated the diffusion of the Al atom during oxidation,leading to the formation of a dense Al_(2)O_(3)layer on the surface.The mechanism of improvement in the oxidation resistance of the as-deposited Ti45Al8Nb alloy via LSP was discussed.展开更多
[ Objective] Study on the photosynthesis and fluorescence characteristics in peony under water stress. [ Method ] The two peony varieties Huhong and Luoyanghong were treated by different water stress for determining t...[ Objective] Study on the photosynthesis and fluorescence characteristics in peony under water stress. [ Method ] The two peony varieties Huhong and Luoyanghong were treated by different water stress for determining the photosynthesis and fluorescence characteristics. [ Result ] With the aggravation of water stress, the net photosynthetic rate (Pn) and stomatal conductance (Gs) decreased, while the intercellular CO2 concentration (Ci) increased. Drought could decrease Pn, constrain gas exchange and change the daily photosynthesis. Fo of peony leaf increased and Fv/Fm decreased under water stress especially water logging, causing the inactivation of the PS II reaction center, and the chlorophyll fluorescence characters gradually recovered until afternoon. [ Conclusion] The 75% soil relative water content (SRWC) is the best condition for growth of peony. Compared with drought, water logging is more unfit for the growth of peony. For the two varieties, Huhong assumed more tolerance to drought, accordingly more adaptability.展开更多
Because of the generated residual stress after applying cold expanded technique with split sleeve, fatigue life of joint hole in aircraft can be distinguishedly prolonged. To study residual stress around cold expan...Because of the generated residual stress after applying cold expanded technique with split sleeve, fatigue life of joint hole in aircraft can be distinguishedly prolonged. To study residual stress around cold expanded hole, some hypothesises such as small deformation in radial symmetry, plane stress, plane strain, uniform distribution pressure have been presented in most papers. However, accurate result cannot be gained based on these hypothesises. In order to overcome the shortage, a 3 dimensional model is proposed which takes into consideration of the effect on split sleeve and the different distribution pressure along thick direction of specimen. The stress distributions of cold expanded hole with split sleeve under loading or unloading, as well as under fatigue tension have been stu died. To reveal the advantage of cold expanding process with split sleeve, the expansion without split sleeve has also been studied and compared with the former.展开更多
The contents of total phenolics and extractable condensed tannins in the leaves, twigs and stem bark of Canarium album were determined. The structural heterogeneity of condensed tannins from stem bark was characterize...The contents of total phenolics and extractable condensed tannins in the leaves, twigs and stem bark of Canarium album were determined. The structural heterogeneity of condensed tannins from stem bark was characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and nuclear magnetic resonance (NMR) analyses The results show the predominance of signals representative of procyanidins and prodelphinidins. In addition, epicatechin and epigallocatechin polymers with galloylated procyanidin or prodelphinidin were also observed. The tannins were screened for their potential antioxidant activities using l,l-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing/antioxidant power (FRAP) model systems. Tannins extracted from leaves, twigs and stem bark all showed a very good DPPH radical scavenging activity and ferric reducing power.展开更多
[Objective] This study aimed to establish an identification system for drought-resistance in wheat by using near-infrared diffuse reflectance spectroscopy. [Method] In 2006-2007, 36 wheat varieties with different drou...[Objective] This study aimed to establish an identification system for drought-resistance in wheat by using near-infrared diffuse reflectance spectroscopy. [Method] In 2006-2007, 36 wheat varieties with different drought resistance were selected and were classified according to their drought resistance grades determined by the Technical Specification of Identification and Evaluation for Drought Resistance in Wheat (GB/T 21127-2007). In addition, the harvested wheat seed samples were spectrally analyzed with FOSS NIRSystems5000 near-infrared spectrum analyzer for grain quality (full spectrum analyzer) and then the forecasted regression equations were established. [Result] After the establishment of a database and validation, dis- criminated functions were obtained. The determination coefficient (RSQ) and coeffi- cients of determination for cross validation (1-VR) in the discriminant function built with seed samples from water stress area were 0.846 0 and 0.781 8, respectively, which indicated that the consistency between drought resistance and spectral charac- teristics in wheat varieties was good, and there was high correlation between the near-infrared diffuse reflectance spectra of seeds and the drought resistance in wheat. [Conclusiou] Under water stress condition, it is feasible to establish a conve- nient, rapid and no-damage identification system for the drought resistance in wheat by using the near-infrared diffuse reflectance spectrum technique to scan wheat seeds.展开更多
120 azuki bean germplasms from different regions of China were selected for drought-resistance. Results showed that there is a significant positive correlation between drought-resistance and photooxidation-resistance....120 azuki bean germplasms from different regions of China were selected for drought-resistance. Results showed that there is a significant positive correlation between drought-resistance and photooxidation-resistance. So, the detecting technique for photooxidation-resistance should be suggested as a reference method to select the drought-resistance germplasms in azuki bean.展开更多
[Objective] This study aimed to compare the contents of total alkaloids in different solvent extracts from climbing groundsel herb produced in Guizhou Province and their antibacterial activity. [Method] The content of...[Objective] This study aimed to compare the contents of total alkaloids in different solvent extracts from climbing groundsel herb produced in Guizhou Province and their antibacterial activity. [Method] The content of total alkaloids was measured by UV spectrophotometry, and Oxford cups were used to investigate the antibacterial activity of each solvent extract. [Result] There was a good linear correlation between the absorbance measured by the UV spectrophotometer and the content of total al- kaloids within the concentration of 0.011 0-0.054 8 mg/ml at 207 nm, and the re- gression equation was Y=23.654X+0.021, R=0. 999 7 and the average recovery rate was 99.2%. The contents of total alkaloids in 60% ethanol extract, 95% ethanol and water ethanol were 38.71, 52.25 and 60.50 mg/g, respectively. The 60% ethanol ex- tract had strong antibacterial activity against Streptococcus pneumoniae, Staphylo- coccus aureus and Escherichia coil; the water extractive had stronger antibacterial activity against Escherichia colr, 95% ethanol extract had weak antibacterial activity, with no inhibition of Pseudomonas aeruginosa. [Conclusion] This method is accurate, simple, with good repeatability, which can be used for the determination of alkaloids content of the climbing groundsel herb; there is no positive correlation between the content of total alkaloids in different solvent extracts of climbing groundsel herb and their antibacterial activity.展开更多
[Objective] The aim was to build an evaluation method rapidly identifying wheat drought tolerance with near infrared diffuse reflectance spectroscopy. [Method] In the research, 36 wheat varieties in 2007-2009 were cho...[Objective] The aim was to build an evaluation method rapidly identifying wheat drought tolerance with near infrared diffuse reflectance spectroscopy. [Method] In the research, 36 wheat varieties in 2007-2009 were chosen and drought-tolerance degrees of wheat were graded and identified according to Winter-wheat Drought Tol- erance Evaluation Technical Standards (GB/T 21127-2007), and harvest wheat grains underwent spectrum collection, with a full-spectrum analyzer, to establish a database. [Result] Based on qualitative analysis and full-spectrum correlation research, the coef- ficient of determination (RSQ) and cross-validation coefficient of determination (1-VR) were concluded at 0.697 5 and 0.600 2, showing near-infrared diffuse reflectance spectroscopy is of significant differences among wheat varieties and of significant or extremely significant correlation with drought-tolerance indices. [Conclusion] The re- search indicates that to evaluate drought-tolerance of wheat with near-infrared diffuse reflectance spectroscopy is a rapid and feasible way, which is simple, convenient without damages on grains, and of practical values for construction wheat drought-tol- erance evaluation index system and identification of breeding materials.展开更多
Apiotherapy has been applied by Georgian people since time immemorial. Treatment with bee products in Georgia has been described in popular writings. Among bee products, honey and propolis are of most particular inter...Apiotherapy has been applied by Georgian people since time immemorial. Treatment with bee products in Georgia has been described in popular writings. Among bee products, honey and propolis are of most particular interest. Propolis is characterized by antimicrobial, antiviral, antioxidant, anticarcinogenic and analgesic properties. We have studied relationship between optical density and antimicrobial properties. Almost all samples of Georgian propolis are characterized by high optical density and antimicrobial activity. Studies have discovered that optical density was high in the samples of spring season. The maximal absorption values have been observed at a wavelength of 290-310 nm that is characteristic of flavonoids. Also, the sample with a relatively low optical density (Baghdati district) has exhibited antimicrobial activity that is conditioned not by polyphenols, but flavonoids.展开更多
This letter proposes a novel design of a Micro Electro Mechanical System (MEMS) device featuring a metal grating vibratory mierostructure driven by electrostatic force to sense the spatial electric field. Due to the...This letter proposes a novel design of a Micro Electro Mechanical System (MEMS) device featuring a metal grating vibratory mierostructure driven by electrostatic force to sense the spatial electric field. Due to the advantages in slide-film damping and large vibration amplitude, such a device makes atmospheric packaging a low-cost option for practical manufacture. In this letter, we present the operating principles and specifications, the design structure, as well as the finite element simulation. Computational analysis shows that our design obtains good results in device parameters setting, while its simplicity and low-cost features make it an attractive solution for applications.展开更多
ZnO nanoparticles were first encapsulated in submicron PS hollow microspheres through two-step swelling process of core-shell structured PMMA/PS (PMMA: polymethyl methao- rylate) microspheres in acid-alkali solutio...ZnO nanoparticles were first encapsulated in submicron PS hollow microspheres through two-step swelling process of core-shell structured PMMA/PS (PMMA: polymethyl methao- rylate) microspheres in acid-alkali solution, and the ZnO precursors, i.e. the ethanol solu- tions of (CHaCOO)2Zn and LiOH. The transmission electron microscope, X-ray diffraction, and thermogravimetric analysis results show that the feeding order of ethanol solutions of (CH3COO)2Zn and LiOH in the second swelling step has great influence on the loading efficiency and the size of ZnO nanoparticles, but little on their crystal form. The photolumi- nescence and UV-Vis absorption behavior of ZnO/PS microspheres show that the PS shell can effectively avoid the fluorescence quenching effect.展开更多
SalNa (sodium salinomycin) reacts with divalent transition metal ions of Co(II), Ni(II), Cu(II) and Zn(II) to produce novel compounds characterized by various spectroscopic methods. The interaction of metal ...SalNa (sodium salinomycin) reacts with divalent transition metal ions of Co(II), Ni(II), Cu(II) and Zn(II) to produce novel compounds characterized by various spectroscopic methods. The interaction of metal (II) ions with SalNa results in the formation of mononuclear complexes of a general composition of [M(Sal)2·(H2O)2] nH2O (n = 0 or 2) where the divalent cations replace Na~ ions from the cavity of initial compound. The new compounds (disalinomycinates) possess an enhanced antibacterial activity against Gram-positive microorganisms as compared to both SalNa and SalH (salinomycinic acid), respectively. The metal (II) complexes manifest strong concentration dependent cytotoxic effect in experiments using human leukemia cell lines. The complexes of Co0I) and Cu(lI) proved to exert superior activity as compared to the Ni(II) and Zn(II) analogues and are much more cytotoxic than SalNa and SalH. Further studies should be conducted to determine the therapeutic indexes of the new compounds.展开更多
The goal of this work is to investigate the seismic behaviour of plan-asymmetric structures by considering the least seismic-resistant directions and the spatial features of the seismic event. New tools for the analys...The goal of this work is to investigate the seismic behaviour of plan-asymmetric structures by considering the least seismic-resistant directions and the spatial features of the seismic event. New tools for the analysis of the seismic behaviour of plan-asymmetric structures are herein presented and the concepts of "Polar Spectrum" and limit domains are discussed. In particular, the capacity of the structure is described by using the limit domains based on the Non Linear Static Procedures, while the seismic demand is analysed by introducing a new representation of the spectral response. This representation is based on the construction of a spectral surface obtained by the spectral seismic response for different in-plan directions and the in-plan projection of this surface is herein defined "Polar Spectrum". The obtained results for two benchmark structures, verified by means of non-linear incremental dynamic analyses, have pointed out that non-linear static analyses, carried out for different in-plan directions of the incoming seismic action, have allowed us to accurately evaluate the least seismic resistant directions.展开更多
The FlaA gene from Vibrio harveyi marker, was cloned into the eukaryotic expression with a short nucleotide sequence encoding the Flag vector pcDNA3.1(+) (designated as pcFlaA). Ninety grouper (Epinephelus awoar...The FlaA gene from Vibrio harveyi marker, was cloned into the eukaryotic expression with a short nucleotide sequence encoding the Flag vector pcDNA3.1(+) (designated as pcFlaA). Ninety grouper (Epinephelus awoara) were separated into three equal size groups. An experimental group was immunized with pcFlaA, Control I group was immunized with the vector pcDNA3.1(+), and Control 1I group was immunized with PBS. The expression of pcFlaA mRNA and protein was examined using reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. We also evaluated the immunogenicity and protective efficacy of pcFlaA against V. harveyi by measuring the lymphocyte proliferation response and serum levels of specific antibody and conducting a bacterial challenge test. We successfully transfected the fish muscle with pcFlaA. The pcFlaA mRNA and protein was expressed in the muscle cells for up to one month following injection. The proliferation response of lymphocytes in fish immunized with pcFlaA was significantly higher than in control group II. Furthermore, the immunized fish generated specific antibody. The vaccination also resulted in significantly higher survival during the bacterial challenge test.展开更多
To estimate the antioxidant activities of Peanut polypeptides (PPs) by using a chemiluminescence (CL) method in vitro. The scavenging ability of PPs on superoxide anion, hydroxide radical, and hydrogen peroxide wa...To estimate the antioxidant activities of Peanut polypeptides (PPs) by using a chemiluminescence (CL) method in vitro. The scavenging ability of PPs on superoxide anion, hydroxide radical, and hydrogen peroxide was determined by the Pyrogallol-Luminol system, the CuSO4-Phen-Vc-H2O2 system, and the luminol-H2O2 system, respectively. DNA damage preventing the effect of PPs was determined by the CuSO4-Phen-Vc-H2O2-DNA CL system. The results shows that PPs had good effect on the scavenging ability of superoxide anion (IC50=9.68±0.12 mg/ml). PPs could scavenge hydroxide radical effectively (the IC50 value was 46.06±0.08 μg/ml). PPs had a good scavenging ability on hydrogen peroxide, which had a relatively low IC50 value (0.17±0.07 mg/ml). PPs (the IC50 value was0.72±0.11 mg/ml) were powerful on the DNA damage preventing effect. PPs possesses a good scavenging potency on ROS in different systems, but different results exist in different systems.展开更多
基金supported by the Class Ⅲ Peak Discipline of Shanghai,China-Materials Science and Engineering(High-Energy Beam Intelligent Processing and Green Manufacturing).
文摘Laser shock peening(LSP)was used to enhance the high-temperature oxidation resistance of laser melting deposited Ti45Al8Nb alloy.The microstructure and high-temperature oxidation behavior of the as-deposited Ti45Al8Nb alloy before and after LSP were investigated by scanning electron microscopy,X-ray diffraction,and electron backscatter diffraction.The results indicated that the rate of mass gain in the as-deposited sample after LSP exhibited a decrease when exposed to an oxidation temperature of 900℃,implying that LSP-treated samples exhibited superior oxidation resistance at high temperatures.A gradient structure with a fine-grain layer,a deformed-grain layer,and a coarse-grain layer was formed in the LSP-treated sample,which facilitated the diffusion of the Al atom during oxidation,leading to the formation of a dense Al_(2)O_(3)layer on the surface.The mechanism of improvement in the oxidation resistance of the as-deposited Ti45Al8Nb alloy via LSP was discussed.
基金the Key Project Foundation of Henan Province (0122012400)the Key Project Foundation of Luoyang City (20011001)~~
文摘[ Objective] Study on the photosynthesis and fluorescence characteristics in peony under water stress. [ Method ] The two peony varieties Huhong and Luoyanghong were treated by different water stress for determining the photosynthesis and fluorescence characteristics. [ Result ] With the aggravation of water stress, the net photosynthetic rate (Pn) and stomatal conductance (Gs) decreased, while the intercellular CO2 concentration (Ci) increased. Drought could decrease Pn, constrain gas exchange and change the daily photosynthesis. Fo of peony leaf increased and Fv/Fm decreased under water stress especially water logging, causing the inactivation of the PS II reaction center, and the chlorophyll fluorescence characters gradually recovered until afternoon. [ Conclusion] The 75% soil relative water content (SRWC) is the best condition for growth of peony. Compared with drought, water logging is more unfit for the growth of peony. For the two varieties, Huhong assumed more tolerance to drought, accordingly more adaptability.
文摘Because of the generated residual stress after applying cold expanded technique with split sleeve, fatigue life of joint hole in aircraft can be distinguishedly prolonged. To study residual stress around cold expanded hole, some hypothesises such as small deformation in radial symmetry, plane stress, plane strain, uniform distribution pressure have been presented in most papers. However, accurate result cannot be gained based on these hypothesises. In order to overcome the shortage, a 3 dimensional model is proposed which takes into consideration of the effect on split sleeve and the different distribution pressure along thick direction of specimen. The stress distributions of cold expanded hole with split sleeve under loading or unloading, as well as under fatigue tension have been stu died. To reveal the advantage of cold expanding process with split sleeve, the expansion without split sleeve has also been studied and compared with the former.
基金Project supported by the National Natural Science Foundation of China (No. 30671646)the Program for New Century Excellent Talents in University, China (No. NCET-07-0725)the Program for Innovative Research Team in Science and Technology in Fujian Province University, China
文摘The contents of total phenolics and extractable condensed tannins in the leaves, twigs and stem bark of Canarium album were determined. The structural heterogeneity of condensed tannins from stem bark was characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and nuclear magnetic resonance (NMR) analyses The results show the predominance of signals representative of procyanidins and prodelphinidins. In addition, epicatechin and epigallocatechin polymers with galloylated procyanidin or prodelphinidin were also observed. The tannins were screened for their potential antioxidant activities using l,l-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing/antioxidant power (FRAP) model systems. Tannins extracted from leaves, twigs and stem bark all showed a very good DPPH radical scavenging activity and ferric reducing power.
基金Supported by the Special Fund for the Industrial Technology System Construction of Modern Agriculture in Wheat(CARS-E-2-36)the Special Fund for Henan Industrial Technology System Construction of Modern Agriculture in Wheat(S2010-10-02)National Support Program for Science and Technology(2011BAD35B03)~~
文摘[Objective] This study aimed to establish an identification system for drought-resistance in wheat by using near-infrared diffuse reflectance spectroscopy. [Method] In 2006-2007, 36 wheat varieties with different drought resistance were selected and were classified according to their drought resistance grades determined by the Technical Specification of Identification and Evaluation for Drought Resistance in Wheat (GB/T 21127-2007). In addition, the harvested wheat seed samples were spectrally analyzed with FOSS NIRSystems5000 near-infrared spectrum analyzer for grain quality (full spectrum analyzer) and then the forecasted regression equations were established. [Result] After the establishment of a database and validation, dis- criminated functions were obtained. The determination coefficient (RSQ) and coeffi- cients of determination for cross validation (1-VR) in the discriminant function built with seed samples from water stress area were 0.846 0 and 0.781 8, respectively, which indicated that the consistency between drought resistance and spectral charac- teristics in wheat varieties was good, and there was high correlation between the near-infrared diffuse reflectance spectra of seeds and the drought resistance in wheat. [Conclusiou] Under water stress condition, it is feasible to establish a conve- nient, rapid and no-damage identification system for the drought resistance in wheat by using the near-infrared diffuse reflectance spectrum technique to scan wheat seeds.
文摘120 azuki bean germplasms from different regions of China were selected for drought-resistance. Results showed that there is a significant positive correlation between drought-resistance and photooxidation-resistance. So, the detecting technique for photooxidation-resistance should be suggested as a reference method to select the drought-resistance germplasms in azuki bean.
基金Supported by the Clinical Scientific Research Capacity Construction Program for Country-level Hospitals of Department of Health,Guizhou Province(gzwxky2011-1-022)~~
文摘[Objective] This study aimed to compare the contents of total alkaloids in different solvent extracts from climbing groundsel herb produced in Guizhou Province and their antibacterial activity. [Method] The content of total alkaloids was measured by UV spectrophotometry, and Oxford cups were used to investigate the antibacterial activity of each solvent extract. [Result] There was a good linear correlation between the absorbance measured by the UV spectrophotometer and the content of total al- kaloids within the concentration of 0.011 0-0.054 8 mg/ml at 207 nm, and the re- gression equation was Y=23.654X+0.021, R=0. 999 7 and the average recovery rate was 99.2%. The contents of total alkaloids in 60% ethanol extract, 95% ethanol and water ethanol were 38.71, 52.25 and 60.50 mg/g, respectively. The 60% ethanol ex- tract had strong antibacterial activity against Streptococcus pneumoniae, Staphylo- coccus aureus and Escherichia coil; the water extractive had stronger antibacterial activity against Escherichia colr, 95% ethanol extract had weak antibacterial activity, with no inhibition of Pseudomonas aeruginosa. [Conclusion] This method is accurate, simple, with good repeatability, which can be used for the determination of alkaloids content of the climbing groundsel herb; there is no positive correlation between the content of total alkaloids in different solvent extracts of climbing groundsel herb and their antibacterial activity.
基金Supported by National Wheat Industry System(CARS-E-2-36)Henan Wheat Industry System(S2010-10-02)National Science and Technology Support Plan(2011BAD35B-03)~~
文摘[Objective] The aim was to build an evaluation method rapidly identifying wheat drought tolerance with near infrared diffuse reflectance spectroscopy. [Method] In the research, 36 wheat varieties in 2007-2009 were chosen and drought-tolerance degrees of wheat were graded and identified according to Winter-wheat Drought Tol- erance Evaluation Technical Standards (GB/T 21127-2007), and harvest wheat grains underwent spectrum collection, with a full-spectrum analyzer, to establish a database. [Result] Based on qualitative analysis and full-spectrum correlation research, the coef- ficient of determination (RSQ) and cross-validation coefficient of determination (1-VR) were concluded at 0.697 5 and 0.600 2, showing near-infrared diffuse reflectance spectroscopy is of significant differences among wheat varieties and of significant or extremely significant correlation with drought-tolerance indices. [Conclusion] The re- search indicates that to evaluate drought-tolerance of wheat with near-infrared diffuse reflectance spectroscopy is a rapid and feasible way, which is simple, convenient without damages on grains, and of practical values for construction wheat drought-tol- erance evaluation index system and identification of breeding materials.
文摘Apiotherapy has been applied by Georgian people since time immemorial. Treatment with bee products in Georgia has been described in popular writings. Among bee products, honey and propolis are of most particular interest. Propolis is characterized by antimicrobial, antiviral, antioxidant, anticarcinogenic and analgesic properties. We have studied relationship between optical density and antimicrobial properties. Almost all samples of Georgian propolis are characterized by high optical density and antimicrobial activity. Studies have discovered that optical density was high in the samples of spring season. The maximal absorption values have been observed at a wavelength of 290-310 nm that is characteristic of flavonoids. Also, the sample with a relatively low optical density (Baghdati district) has exhibited antimicrobial activity that is conditioned not by polyphenols, but flavonoids.
基金Supported by the National Natural Science Foundation of China (No.60172001).
文摘This letter proposes a novel design of a Micro Electro Mechanical System (MEMS) device featuring a metal grating vibratory mierostructure driven by electrostatic force to sense the spatial electric field. Due to the advantages in slide-film damping and large vibration amplitude, such a device makes atmospheric packaging a low-cost option for practical manufacture. In this letter, we present the operating principles and specifications, the design structure, as well as the finite element simulation. Computational analysis shows that our design obtains good results in device parameters setting, while its simplicity and low-cost features make it an attractive solution for applications.
文摘ZnO nanoparticles were first encapsulated in submicron PS hollow microspheres through two-step swelling process of core-shell structured PMMA/PS (PMMA: polymethyl methao- rylate) microspheres in acid-alkali solution, and the ZnO precursors, i.e. the ethanol solu- tions of (CHaCOO)2Zn and LiOH. The transmission electron microscope, X-ray diffraction, and thermogravimetric analysis results show that the feeding order of ethanol solutions of (CH3COO)2Zn and LiOH in the second swelling step has great influence on the loading efficiency and the size of ZnO nanoparticles, but little on their crystal form. The photolumi- nescence and UV-Vis absorption behavior of ZnO/PS microspheres show that the PS shell can effectively avoid the fluorescence quenching effect.
文摘SalNa (sodium salinomycin) reacts with divalent transition metal ions of Co(II), Ni(II), Cu(II) and Zn(II) to produce novel compounds characterized by various spectroscopic methods. The interaction of metal (II) ions with SalNa results in the formation of mononuclear complexes of a general composition of [M(Sal)2·(H2O)2] nH2O (n = 0 or 2) where the divalent cations replace Na~ ions from the cavity of initial compound. The new compounds (disalinomycinates) possess an enhanced antibacterial activity against Gram-positive microorganisms as compared to both SalNa and SalH (salinomycinic acid), respectively. The metal (II) complexes manifest strong concentration dependent cytotoxic effect in experiments using human leukemia cell lines. The complexes of Co0I) and Cu(lI) proved to exert superior activity as compared to the Ni(II) and Zn(II) analogues and are much more cytotoxic than SalNa and SalH. Further studies should be conducted to determine the therapeutic indexes of the new compounds.
文摘The goal of this work is to investigate the seismic behaviour of plan-asymmetric structures by considering the least seismic-resistant directions and the spatial features of the seismic event. New tools for the analysis of the seismic behaviour of plan-asymmetric structures are herein presented and the concepts of "Polar Spectrum" and limit domains are discussed. In particular, the capacity of the structure is described by using the limit domains based on the Non Linear Static Procedures, while the seismic demand is analysed by introducing a new representation of the spectral response. This representation is based on the construction of a spectral surface obtained by the spectral seismic response for different in-plan directions and the in-plan projection of this surface is herein defined "Polar Spectrum". The obtained results for two benchmark structures, verified by means of non-linear incremental dynamic analyses, have pointed out that non-linear static analyses, carried out for different in-plan directions of the incoming seismic action, have allowed us to accurately evaluate the least seismic resistant directions.
基金Supported by Fujian Science and Technology Innovation Foundation for Young Scientists (No.2006F3096)Scientific Research Foundation of Jimei University
文摘The FlaA gene from Vibrio harveyi marker, was cloned into the eukaryotic expression with a short nucleotide sequence encoding the Flag vector pcDNA3.1(+) (designated as pcFlaA). Ninety grouper (Epinephelus awoara) were separated into three equal size groups. An experimental group was immunized with pcFlaA, Control I group was immunized with the vector pcDNA3.1(+), and Control 1I group was immunized with PBS. The expression of pcFlaA mRNA and protein was examined using reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. We also evaluated the immunogenicity and protective efficacy of pcFlaA against V. harveyi by measuring the lymphocyte proliferation response and serum levels of specific antibody and conducting a bacterial challenge test. We successfully transfected the fish muscle with pcFlaA. The pcFlaA mRNA and protein was expressed in the muscle cells for up to one month following injection. The proliferation response of lymphocytes in fish immunized with pcFlaA was significantly higher than in control group II. Furthermore, the immunized fish generated specific antibody. The vaccination also resulted in significantly higher survival during the bacterial challenge test.
文摘To estimate the antioxidant activities of Peanut polypeptides (PPs) by using a chemiluminescence (CL) method in vitro. The scavenging ability of PPs on superoxide anion, hydroxide radical, and hydrogen peroxide was determined by the Pyrogallol-Luminol system, the CuSO4-Phen-Vc-H2O2 system, and the luminol-H2O2 system, respectively. DNA damage preventing the effect of PPs was determined by the CuSO4-Phen-Vc-H2O2-DNA CL system. The results shows that PPs had good effect on the scavenging ability of superoxide anion (IC50=9.68±0.12 mg/ml). PPs could scavenge hydroxide radical effectively (the IC50 value was 46.06±0.08 μg/ml). PPs had a good scavenging ability on hydrogen peroxide, which had a relatively low IC50 value (0.17±0.07 mg/ml). PPs (the IC50 value was0.72±0.11 mg/ml) were powerful on the DNA damage preventing effect. PPs possesses a good scavenging potency on ROS in different systems, but different results exist in different systems.