为了寻找冷冻液中最适合的抗冷冻蛋白Ⅲ(AFPⅢ)添加浓度,并探索AFPⅢ对玻璃化冷冻卵母细胞、活性氧(ROS)及其DNA损伤的保护作用进行评价。本研究分别在冷冻液中添加250、500、750、1000、1200 ng/m L AFPⅢ,通过冷冻液处理和冷冻-复苏...为了寻找冷冻液中最适合的抗冷冻蛋白Ⅲ(AFPⅢ)添加浓度,并探索AFPⅢ对玻璃化冷冻卵母细胞、活性氧(ROS)及其DNA损伤的保护作用进行评价。本研究分别在冷冻液中添加250、500、750、1000、1200 ng/m L AFPⅢ,通过冷冻液处理和冷冻-复苏筛选出最合适的AFPⅢ添加浓度,荧光探针检测卵母细胞中活性氧的含量,彗星电泳检测卵母细胞DNA的变化。结果显示,冷冻液中添加500 ng/m L AFPⅢ对玻璃化冷冻猪GV期卵母细胞的保护效果最佳;冷冻液中添加AFPⅢ的冷冻组ROS含量显著低于冷冻对照组(P<0.05);添加AFPⅢ的冷冻组尾部DNA百分比(Tail DNA%)和Olive尾矩值(OTM)显著低于冷冻对照组(P<0.05)。结果表明,AFPⅢ可以提高玻璃化冷冻后卵母细胞的体外发育能力,降低冷冻-复苏后卵母细胞ROS含量,缓解玻璃化冷冻引起的卵母细胞DNA损伤。展开更多
This study was to research the cryopreservation effect of plant antifieeze proteins(AFPs) on day 7 porcine expanded and hatched blastocysts that were frozen in 1.5 M glycerol by conventional slow freezing method. The ...This study was to research the cryopreservation effect of plant antifieeze proteins(AFPs) on day 7 porcine expanded and hatched blastocysts that were frozen in 1.5 M glycerol by conventional slow freezing method. The developmental rates of porcine embryos frozen with 0.5 mg/ml AFPs in freeze medium and cultured for 12 and 24 hours in vitro are 25% and 0%respectively. With the concentration of AFPs increased to 5 mg/ml, the corresponding values became 80% and 40%. The hatched rates for porcine embryos frozen with 0.5 mg/ml and 5 mg/ml of AFPs and cultured for 24 hours in vitro are 0% and 20% respectively..The developmental and hatched rate of the contnrol are all 0%(0/4). These results indicate that the survival rates of porcine expanded and hatched blastocyst can be improved by supplementing freeze medium with plant AFPs.展开更多
文摘为了寻找冷冻液中最适合的抗冷冻蛋白Ⅲ(AFPⅢ)添加浓度,并探索AFPⅢ对玻璃化冷冻卵母细胞、活性氧(ROS)及其DNA损伤的保护作用进行评价。本研究分别在冷冻液中添加250、500、750、1000、1200 ng/m L AFPⅢ,通过冷冻液处理和冷冻-复苏筛选出最合适的AFPⅢ添加浓度,荧光探针检测卵母细胞中活性氧的含量,彗星电泳检测卵母细胞DNA的变化。结果显示,冷冻液中添加500 ng/m L AFPⅢ对玻璃化冷冻猪GV期卵母细胞的保护效果最佳;冷冻液中添加AFPⅢ的冷冻组ROS含量显著低于冷冻对照组(P<0.05);添加AFPⅢ的冷冻组尾部DNA百分比(Tail DNA%)和Olive尾矩值(OTM)显著低于冷冻对照组(P<0.05)。结果表明,AFPⅢ可以提高玻璃化冷冻后卵母细胞的体外发育能力,降低冷冻-复苏后卵母细胞ROS含量,缓解玻璃化冷冻引起的卵母细胞DNA损伤。
文摘This study was to research the cryopreservation effect of plant antifieeze proteins(AFPs) on day 7 porcine expanded and hatched blastocysts that were frozen in 1.5 M glycerol by conventional slow freezing method. The developmental rates of porcine embryos frozen with 0.5 mg/ml AFPs in freeze medium and cultured for 12 and 24 hours in vitro are 25% and 0%respectively. With the concentration of AFPs increased to 5 mg/ml, the corresponding values became 80% and 40%. The hatched rates for porcine embryos frozen with 0.5 mg/ml and 5 mg/ml of AFPs and cultured for 24 hours in vitro are 0% and 20% respectively..The developmental and hatched rate of the contnrol are all 0%(0/4). These results indicate that the survival rates of porcine expanded and hatched blastocyst can be improved by supplementing freeze medium with plant AFPs.
