Purpose: The purpose of the study is to investigate whether analysis of specific antibody synthesis can aid the diagnosis of herpes keratitis. Methods: Aqueous humor was collected from 39 patients with presumed recurr...Purpose: The purpose of the study is to investigate whether analysis of specific antibody synthesis can aid the diagnosis of herpes keratitis. Methods: Aqueous humor was collected from 39 patients with presumed recurrent herpes keratitis, including 23 consulting for keratitis and 16 patients scheduled for penetrating keratoplasty. Local antibody production was ascertained by analysis of paired aqueous humor/serum samples, using a modified micro-ELISA technique. Results: Local production of antibodies was found in 32 patients (82% ): anti-herpes simplex virus (HSV) antibodies in 26 (67% ) and anti-varicella zoster virus (VZV) antibodies in 11 (28% ). Twenty of 23 patients with active keratitis (87% ), and 12 of 16 undergoing keratoplasty (75% ), tested positive. Five patients had local production of both anti-HSV and anti-VZV antibodies, whereas seven patients tested negative. Local antibody production was significantly associated with intraocular inflammation (P< 0.05), corneal neovascularisation (P< 0.05), and positive response to anti-viral treatment (P< 0.05). No complications were encountered in sampling aqueous humor. Conclusions: Assessment of local anti- HSV and-VZV antibody production is a safe and reliable diagnostic procedure for recurrent herpes keratitis. It might be particularly helpful in patients presenting with intraocular inflammation and neovascularisation since it discriminates between herpes and non-herpes pathologies and may therefore be useful for preventive and therapeutic strategies.展开更多
Nucleoside analogues have been the mainstay of clinical treatment of herpes simplex virus 1 (HSV-1) infections since their development. However, the emergence of drug resistant strains has underlined the urgency of th...Nucleoside analogues have been the mainstay of clinical treatment of herpes simplex virus 1 (HSV-1) infections since their development. However, the emergence of drug resistant strains has underlined the urgency of the discovery of novel anti-HSV-1 drugs. Natural products, which provided many novel drug leads, are known to be an important source of anti-HSV-1 agents. Herein, we present an overview of natural products with anti-HSV-1 activities isolated from a variety of plants reported in recent years. Several different compounds, mainly belonging to the three groups of polysaccharides, polyphenols and terpenes, showed antiviral effects against HSV-1, indicating their potential to be promising anti-HSV-1 agents.展开更多
最新的两项研究结果显示,两种疫苗有助于妇女对性传播疾病(STDs)和宫颈癌的预防。研究人员在随机的双盲对照试验中发现,人乳头状瘤病毒(HPV)-16疫苗对此前未曾暴露过的妇女的预防效果可高达100％。 Laura A.Koutsky及其同事进行的研究表...最新的两项研究结果显示,两种疫苗有助于妇女对性传播疾病(STDs)和宫颈癌的预防。研究人员在随机的双盲对照试验中发现,人乳头状瘤病毒(HPV)-16疫苗对此前未曾暴露过的妇女的预防效果可高达100％。 Laura A.Koutsky及其同事进行的研究表明,经HPV-16疫苗处理后。展开更多
文摘Purpose: The purpose of the study is to investigate whether analysis of specific antibody synthesis can aid the diagnosis of herpes keratitis. Methods: Aqueous humor was collected from 39 patients with presumed recurrent herpes keratitis, including 23 consulting for keratitis and 16 patients scheduled for penetrating keratoplasty. Local antibody production was ascertained by analysis of paired aqueous humor/serum samples, using a modified micro-ELISA technique. Results: Local production of antibodies was found in 32 patients (82% ): anti-herpes simplex virus (HSV) antibodies in 26 (67% ) and anti-varicella zoster virus (VZV) antibodies in 11 (28% ). Twenty of 23 patients with active keratitis (87% ), and 12 of 16 undergoing keratoplasty (75% ), tested positive. Five patients had local production of both anti-HSV and anti-VZV antibodies, whereas seven patients tested negative. Local antibody production was significantly associated with intraocular inflammation (P< 0.05), corneal neovascularisation (P< 0.05), and positive response to anti-viral treatment (P< 0.05). No complications were encountered in sampling aqueous humor. Conclusions: Assessment of local anti- HSV and-VZV antibody production is a safe and reliable diagnostic procedure for recurrent herpes keratitis. It might be particularly helpful in patients presenting with intraocular inflammation and neovascularisation since it discriminates between herpes and non-herpes pathologies and may therefore be useful for preventive and therapeutic strategies.
基金Joint funds of National Natural Science Foundation of China (U0632010)
文摘Nucleoside analogues have been the mainstay of clinical treatment of herpes simplex virus 1 (HSV-1) infections since their development. However, the emergence of drug resistant strains has underlined the urgency of the discovery of novel anti-HSV-1 drugs. Natural products, which provided many novel drug leads, are known to be an important source of anti-HSV-1 agents. Herein, we present an overview of natural products with anti-HSV-1 activities isolated from a variety of plants reported in recent years. Several different compounds, mainly belonging to the three groups of polysaccharides, polyphenols and terpenes, showed antiviral effects against HSV-1, indicating their potential to be promising anti-HSV-1 agents.
