目的探讨星形细胞瘤中抗原处理相关转运蛋白(transporter associated with antigen processing,TAP)分子表达及其临床意义,探讨星形细胞瘤的治疗策略。方法采用免疫组织化学技术对50例星形细胞瘤患者肿瘤组织和15例来自于脑外伤内减压...目的探讨星形细胞瘤中抗原处理相关转运蛋白(transporter associated with antigen processing,TAP)分子表达及其临床意义,探讨星形细胞瘤的治疗策略。方法采用免疫组织化学技术对50例星形细胞瘤患者肿瘤组织和15例来自于脑外伤内减压手术、高血压脑出血及脑血管畸形手术中所取的正常脑组织中TAP的表达进行了检测。结果星形细胞瘤组27例TAP蛋白表达阳性,TAP蛋白阳性细胞百分率为0·291±0·2724;正常组全部表现为TAP蛋白表达阳性,TAP蛋白阳性细胞百分率为0·584±0·2169(P<0·05)。低级别星形细胞瘤(Ⅰ级和Ⅱ级)25例,TAP蛋白平均阳性细胞百分率为0·374±0·2913;高级别星形细胞瘤(Ⅲ级和Ⅳ级)25例,TAP蛋白平均阳性细胞百分率为0·228±0·2008(P<0·05)。结论星形细胞瘤患者存在TAP分子低表达,这可能促使星形细胞瘤患者肿瘤细胞逃避免疫监视,TAP蛋白缺失率有可能成为预测脑星形细胞瘤的发生及其恶性程度的指标之一。展开更多
Objective:To investigate the changes of calcium transport protein activities of aortic vascular smooth muscle cell in the progress of hypertension,evaluate the effects of irbesartan on them,then to elucidate the relat...Objective:To investigate the changes of calcium transport protein activities of aortic vascular smooth muscle cell in the progress of hypertension,evaluate the effects of irbesartan on them,then to elucidate the relationship between cell calcium transport protein and hypertension.Methods:Sixteen sixteen-week-old SHR were randomized into irbesartan treated group(IBT group,n=8)and distilled water group(DW group,n=8),and Wistar-Kyoto rats(WKY)as normal controls.Irbesartan and appropriate amounts of distilled water were administered respectively for 14 weeks by gavage.Blood pressure of caudal artery was examined before and after treatment,activities of Na+-K+-ATPase and Ca 2+-Mg 2+-ATPase of thoracic vascular aorta smooth muscle cell were measured.Results:Blood pressure in IBT group was much lower than that in DW group(129.17±3.71)vs(186.50±6.06),P<0.01).Compared with DW group,activities of Na+-K+-ATPase and Ca 2+-Mg 2+-ATPase of thoracic vascular aorta smooth muscle cell were remarkedly higher in both IBT group and WKY group(5.56±0.92)vs(2.91±0.56),P<0.01;(4.83±0.86)vs(2.76±0.43),P<0.01),(5.98±0.63)vs(2.91±0.56),P<0.01;(4.94±0.84)vs(2.76±0.43),P<0.01),while there was a slight difference between IBT group and WKY group(P>0.05).Activities of Na+-K+-ATPase and Ca 2-Mg 2+-ATPase of thoracic aorta smooth muscle cel were negatively relative to blood pressure(r=-0.446、r=-0.387,P<0.01).Conclusion:The changes of calcium transport protein activities of aortic vascular smooth muscle cell play an important role in the development of hypertension.Irbesartan may effectively inhibit the progress of hypertension by elevating calcium transport protein activities of vascular smooth muscel cell.展开更多
文摘目的探讨星形细胞瘤中抗原处理相关转运蛋白(transporter associated with antigen processing,TAP)分子表达及其临床意义,探讨星形细胞瘤的治疗策略。方法采用免疫组织化学技术对50例星形细胞瘤患者肿瘤组织和15例来自于脑外伤内减压手术、高血压脑出血及脑血管畸形手术中所取的正常脑组织中TAP的表达进行了检测。结果星形细胞瘤组27例TAP蛋白表达阳性,TAP蛋白阳性细胞百分率为0·291±0·2724;正常组全部表现为TAP蛋白表达阳性,TAP蛋白阳性细胞百分率为0·584±0·2169(P<0·05)。低级别星形细胞瘤(Ⅰ级和Ⅱ级)25例,TAP蛋白平均阳性细胞百分率为0·374±0·2913;高级别星形细胞瘤(Ⅲ级和Ⅳ级)25例,TAP蛋白平均阳性细胞百分率为0·228±0·2008(P<0·05)。结论星形细胞瘤患者存在TAP分子低表达,这可能促使星形细胞瘤患者肿瘤细胞逃避免疫监视,TAP蛋白缺失率有可能成为预测脑星形细胞瘤的发生及其恶性程度的指标之一。
文摘Objective:To investigate the changes of calcium transport protein activities of aortic vascular smooth muscle cell in the progress of hypertension,evaluate the effects of irbesartan on them,then to elucidate the relationship between cell calcium transport protein and hypertension.Methods:Sixteen sixteen-week-old SHR were randomized into irbesartan treated group(IBT group,n=8)and distilled water group(DW group,n=8),and Wistar-Kyoto rats(WKY)as normal controls.Irbesartan and appropriate amounts of distilled water were administered respectively for 14 weeks by gavage.Blood pressure of caudal artery was examined before and after treatment,activities of Na+-K+-ATPase and Ca 2+-Mg 2+-ATPase of thoracic vascular aorta smooth muscle cell were measured.Results:Blood pressure in IBT group was much lower than that in DW group(129.17±3.71)vs(186.50±6.06),P<0.01).Compared with DW group,activities of Na+-K+-ATPase and Ca 2+-Mg 2+-ATPase of thoracic vascular aorta smooth muscle cell were remarkedly higher in both IBT group and WKY group(5.56±0.92)vs(2.91±0.56),P<0.01;(4.83±0.86)vs(2.76±0.43),P<0.01),(5.98±0.63)vs(2.91±0.56),P<0.01;(4.94±0.84)vs(2.76±0.43),P<0.01),while there was a slight difference between IBT group and WKY group(P>0.05).Activities of Na+-K+-ATPase and Ca 2-Mg 2+-ATPase of thoracic aorta smooth muscle cel were negatively relative to blood pressure(r=-0.446、r=-0.387,P<0.01).Conclusion:The changes of calcium transport protein activities of aortic vascular smooth muscle cell play an important role in the development of hypertension.Irbesartan may effectively inhibit the progress of hypertension by elevating calcium transport protein activities of vascular smooth muscel cell.
