三疣梭子蟹抗溶藻弧菌感染品系与野生蟹形态学、16S rRNA和COⅠ比较研究

本实验测定了三疣梭子蟹抗溶藻弧菌感染品系和野生群体的形态特征,并分析了2个群体的16SrRNA和COⅠ序列特征。结果表明,抗溶藻弧菌感染品系的体重、第一侧齿间距和第二测齿间距形态比例参数显著小于野生蟹,其余比例参数间差异不显著。结果表明,抗溶藻弧菌感染品系在外形特征上与野生蟹有一定差别,但是,所有形态的差异系数都小于1.28,尚未达到亚种水平,该结果与16SrRNA和COⅠ分析结果一致。

Potency of Nannochloropsis oculata as Antibacterial, Antioxidant and Antiviral on Humpback Grouper Infected by Vibrio alginolyticus and Viral Nervous Necrotic

The aim of this study was to explore the potency of N. oculata extracts as antibacterial, antioxidant and antiviral on grouper Cromileptes altivelis （C. altivelis） infected by Vibrio alginolyticus （V. alginolyticus） and Viral Nervous Necrotic （VNN）. Dilution test was used to measure antibacterial activity of N. oculata extracts. Antioxidant activity of extracts was expressed by levels of MDA （Malondialdehyde） and SOD （superoxide dismutase） in the brain and kidneys of fish. Antiviral capability of N. oculata was determined by the expression of cellular immune cells Major Histocompatibility Complex （MHC） Class I for proliferation and inhibition of VNN in blood cells with immunocytochemistry. The results showed that N. oculata extract was able to suppress the growth of V. alginolyticus at a concentration of 40%. Increasing levels of SOD and reducing level of MDA indicated that N. oculata extracts may serve as an antibacterial and antioxidant. Providing cellular response of MHC class I cell expressed on C. altive/is blood cells, N. oculata demonstrated its antiviral activity.