目的研究细胞外信号调节蛋白激酶(extracellular signal regulated kinase,ERK)1/2信号通路参与抗心磷脂抗体(anticardiolipin antibodies,ACA)阳性流产的发病过程,从细胞增殖的角度阐明安子合剂对ERK1/2信号通路的影响。方法采用免疫...目的研究细胞外信号调节蛋白激酶(extracellular signal regulated kinase,ERK)1/2信号通路参与抗心磷脂抗体(anticardiolipin antibodies,ACA)阳性流产的发病过程,从细胞增殖的角度阐明安子合剂对ERK1/2信号通路的影响。方法采用免疫组化方法检测ACA阳性流产模型组、空白对照组、安子合剂低/中/高剂量组和阿司匹林组小鼠母胎界面ERK1、ERK2磷酸化水平。结果JD801形态学统计分析结果显示安子合剂低剂量组ERK1平均光密度值(0.678±0.097)接近空白对照组的平均光密度值(0.727±0.047),差异无统计学意义;模型组、安子合剂中剂量组、高剂量组、阿司匹林组ERK1平均光密度值(0.434±0.066、0.613±0.041、0.487±0.061、0.551±0.056)均较空白对照组明显降低,差异均有统计学意义(P<0.01)。模型组、安子合剂低剂量组、中剂量组、高剂量组、阿司匹林组ERK2平均光密度值(0.430±0.058、0.621±0.041、0.562±0.047、0.449±0.062、0.515±0.045)均较空白对照组(0.708±0.038)明显降低,差异有统计学意义(P<0.01)。结论安子合剂可以通过ERK信号转导通路提高细胞增殖的活性,改善胎盘功能,改变妊娠结局。展开更多
复发性自然流产(RSA)发病率不断上升,是一种影响有生育需求女性身心健康的生殖并发症,随着国家三胎政策的实施,越来越多的女性受到复发性流产的困扰,其家庭幸福指数及新生命的延续受到严重威胁。研究发现一半以上的复发性流产可能与免...复发性自然流产(RSA)发病率不断上升,是一种影响有生育需求女性身心健康的生殖并发症,随着国家三胎政策的实施,越来越多的女性受到复发性流产的困扰,其家庭幸福指数及新生命的延续受到严重威胁。研究发现一半以上的复发性流产可能与免疫因素有关,其中抗磷脂抗体异常是最相关的因素之一。抗凝治疗及免疫抑制及调节治疗可有效提高复发性流产合并抗磷脂抗体异常患者的成功分娩率,然而,是否需要及如何实施治疗仍然是最大的医疗热点。本综述的目的是通过查阅文献总结目前抗磷脂综合征复发性流产的治疗方法,为临床治疗提供参考。The rising incidence of recurrent spontaneous abortion (RSA) is a kind of reproductive complications that affects the physical and mental health of women with reproductive needs. With the implementation of the country’s three-child policy, more and more women are suffering from recurrent abortions, and its family happiness index and the continuation of new life are seriously threatened. Studies have found that more than half of recurrent miscarriages may be related to immune factors, among which abnormal antiphospholipid antibodies are one of the most relevant factors. Anticoagulation therapy, immunosuppression and regulation therapy can effectively improve the successful delivery rate of patients with recurrent abortion complicated with abnormal antiphospholipid antibodies. However, whether and how to implement treatment is still the biggest medical hotspot. The purpose of this review is to summarize the current treatment methods of recurrent abortion in antiphospholipid syndrome by reviewing the literature, and to provide reference for clinical treatment.展开更多
文摘目的研究细胞外信号调节蛋白激酶(extracellular signal regulated kinase,ERK)1/2信号通路参与抗心磷脂抗体(anticardiolipin antibodies,ACA)阳性流产的发病过程,从细胞增殖的角度阐明安子合剂对ERK1/2信号通路的影响。方法采用免疫组化方法检测ACA阳性流产模型组、空白对照组、安子合剂低/中/高剂量组和阿司匹林组小鼠母胎界面ERK1、ERK2磷酸化水平。结果JD801形态学统计分析结果显示安子合剂低剂量组ERK1平均光密度值(0.678±0.097)接近空白对照组的平均光密度值(0.727±0.047),差异无统计学意义;模型组、安子合剂中剂量组、高剂量组、阿司匹林组ERK1平均光密度值(0.434±0.066、0.613±0.041、0.487±0.061、0.551±0.056)均较空白对照组明显降低,差异均有统计学意义(P<0.01)。模型组、安子合剂低剂量组、中剂量组、高剂量组、阿司匹林组ERK2平均光密度值(0.430±0.058、0.621±0.041、0.562±0.047、0.449±0.062、0.515±0.045)均较空白对照组(0.708±0.038)明显降低,差异有统计学意义(P<0.01)。结论安子合剂可以通过ERK信号转导通路提高细胞增殖的活性,改善胎盘功能,改变妊娠结局。
文摘复发性自然流产(RSA)发病率不断上升,是一种影响有生育需求女性身心健康的生殖并发症,随着国家三胎政策的实施,越来越多的女性受到复发性流产的困扰,其家庭幸福指数及新生命的延续受到严重威胁。研究发现一半以上的复发性流产可能与免疫因素有关,其中抗磷脂抗体异常是最相关的因素之一。抗凝治疗及免疫抑制及调节治疗可有效提高复发性流产合并抗磷脂抗体异常患者的成功分娩率,然而,是否需要及如何实施治疗仍然是最大的医疗热点。本综述的目的是通过查阅文献总结目前抗磷脂综合征复发性流产的治疗方法,为临床治疗提供参考。The rising incidence of recurrent spontaneous abortion (RSA) is a kind of reproductive complications that affects the physical and mental health of women with reproductive needs. With the implementation of the country’s three-child policy, more and more women are suffering from recurrent abortions, and its family happiness index and the continuation of new life are seriously threatened. Studies have found that more than half of recurrent miscarriages may be related to immune factors, among which abnormal antiphospholipid antibodies are one of the most relevant factors. Anticoagulation therapy, immunosuppression and regulation therapy can effectively improve the successful delivery rate of patients with recurrent abortion complicated with abnormal antiphospholipid antibodies. However, whether and how to implement treatment is still the biggest medical hotspot. The purpose of this review is to summarize the current treatment methods of recurrent abortion in antiphospholipid syndrome by reviewing the literature, and to provide reference for clinical treatment.
