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氮离子入法筛选ε-聚赖氨酸高产菌株 被引量:8
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作者 丛茂林 许鹏 +2 位作者 谭之磊 莫治文 贾士儒 《现代食品科技》 EI CAS 2009年第5期491-493,506,共4页
研究氮离子注入法诱变选育ε-聚赖氨酸高产菌株。本文从土壤筛选的菌株TS02出发,通过注入不同剂量30keV氮离子,辅以抗性平板法结合抑菌圈法,筛选出了一株ε-聚赖氨酸高产白色链霉菌(Streptomyces albulus)GC11。经多次传代实验结果表明G... 研究氮离子注入法诱变选育ε-聚赖氨酸高产菌株。本文从土壤筛选的菌株TS02出发,通过注入不同剂量30keV氮离子,辅以抗性平板法结合抑菌圈法,筛选出了一株ε-聚赖氨酸高产白色链霉菌(Streptomyces albulus)GC11。经多次传代实验结果表明GC11稳定性良好。摇瓶发酵培养24h,GC11抑菌圈直径为23.20mm,比TS02提高了1.32倍;5L自控发酵罐发酵培养72h,GC11ε-PL产量为4.21g/L,比TS02提高了1.30倍,达到了提高ε-聚赖氨酸产量的目的。 展开更多
关键词 氮离子注入 诱变选育 抗性平板法 Ε-聚赖氨酸
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Influence of superplasticizers on the early-age crack resistance of concrete 被引量:1
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作者 钱春香 陆旺杰 +3 位作者 何智海 范凌志 曲军 郭景强 《Journal of Southeast University(English Edition)》 EI CAS 2012年第1期41-45,共5页
In order to improve the early-age cracking resistance of concrete, different types of superplasticizers are used. Two types of polycarboxlic salt/acid superplasticizers and one retarding naphthalene superplasticizer a... In order to improve the early-age cracking resistance of concrete, different types of superplasticizers are used. Two types of polycarboxlic salt/acid superplasticizers and one retarding naphthalene superplasticizer are selected to investigate the influence of superplasticizers on the early-age cracking resistance of the concrete by using the slab test and the temperature-stress test. The results show that the polycarboxlic salt/acid superplasticizer cannot always improve the cracking resistance capacity of the concrete compared with the naphthalene superplasticizer, which is related to the chemical structure of the polycarboxlic salt/acid superplasticizer. High plastic tensile strength and dynamic elastic modulus at the early stage are beneficial to avoid cracking, and low hydration heat is also helpful. The evolutions of the drying shrilakage stress and the hydration heat temperature stress varying with time can be comprehensively evaluated by means of the slab test and the temperature stress test. 展开更多
关键词 SUPERPLASTICIZER crack resistance slab testmethod temperature-stress test method
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