Genotyping 42 serum samples taken from the pigs in the oubreaks from 2009 to 2013 with RT-PCR and nested RT-PCR, showed more than 80% of samples were positive with Chinese PRRSV clade, and the others were European and...Genotyping 42 serum samples taken from the pigs in the oubreaks from 2009 to 2013 with RT-PCR and nested RT-PCR, showed more than 80% of samples were positive with Chinese PRRSV clade, and the others were European and North American classical PRRSV genotypes. Ten serum samples from unnapprent PRRS herds were examined for antibodies against PRRSV with ELISA and also for PRRSV with RT-PCR. It was clearly that the titer of antibodies against PRRSV by ELISA test could not be used for interpreting PRRSV infection. In despite of PRRS vaccination or non-vaccination, a risk of PRRSV infection and re-infection exist, utilizing RT-PCR in combination with serology will give the producer and veterinarian PRRSV more exact situation in the herds.展开更多
文摘Genotyping 42 serum samples taken from the pigs in the oubreaks from 2009 to 2013 with RT-PCR and nested RT-PCR, showed more than 80% of samples were positive with Chinese PRRSV clade, and the others were European and North American classical PRRSV genotypes. Ten serum samples from unnapprent PRRS herds were examined for antibodies against PRRSV with ELISA and also for PRRSV with RT-PCR. It was clearly that the titer of antibodies against PRRSV by ELISA test could not be used for interpreting PRRSV infection. In despite of PRRS vaccination or non-vaccination, a risk of PRRSV infection and re-infection exist, utilizing RT-PCR in combination with serology will give the producer and veterinarian PRRSV more exact situation in the herds.
