本文采用大孔树脂纯化瓜馥木总黄酮,并评价其抗抑郁活性。以大孔树脂AB-8、HPD-600、HPD-826、X-5和D4006对瓜馥木总黄酮的吸附率和解吸率为指标筛选树脂种类,并对优选树脂的吸附特性和各影响因素进行研究,优化工艺条件。利用小鼠强迫...本文采用大孔树脂纯化瓜馥木总黄酮,并评价其抗抑郁活性。以大孔树脂AB-8、HPD-600、HPD-826、X-5和D4006对瓜馥木总黄酮的吸附率和解吸率为指标筛选树脂种类,并对优选树脂的吸附特性和各影响因素进行研究,优化工艺条件。利用小鼠强迫游泳、小鼠悬尾和小鼠开野实验对瓜馥木总黄酮的抗抑郁活性进行了评价。结果表明AB-8具有较好的吸附率和解吸率,最佳纯化工艺为:上样流速为1.0 m L/min,树脂床用7 BV 5%乙醇除杂,再用8 BV 50%乙醇洗脱,洗脱流速1.0 m L/min,经AB-8大孔树脂纯化1次后,瓜馥木总黄酮含量为57.3%。瓜馥木总黄酮100 mg/kg剂量给药组能明显缩短小鼠强迫游泳和悬尾的不动时间,且该给药组小鼠的自主运动情况与阳性对照和空白之间没有显著差异。AB-8大孔树脂能较好地用于瓜馥木总黄酮的纯化,瓜馥木总黄酮具有明确的抗抑郁活性。展开更多
AIM: To search some compounds that have anti-depressive activity from Helicid WN7-6 and its derivatives, and to explore their structure-function relationship. METHODS: 21 compounds including WN7-6, the lead compound w...AIM: To search some compounds that have anti-depressive activity from Helicid WN7-6 and its derivatives, and to explore their structure-function relationship. METHODS: 21 compounds including WN7-6, the lead compound were screened for antidepressant effect by the behavioral despair models(tail suspension test and the forced swimming test). And to study their stimulative effect on central nerve system(CNS) by mice autonomic activity test. RESULTS: Results shown that 11 of these compounds could decrease immobile time in suspend tail test and 6 compounds had the similar effect in force swimming test, compared with that of control group, the immobile time was obviously reduced (P<0.05, P<0.01). The results of autonomic activity test indicated that only 7 compounds could inhibit the activities of CNS (P<0.05, P<0.01). CONCLUSION: 30%-50% of these derivatives have anti-depressive activities, and much attention should be paid to further researches.展开更多
文摘本文采用大孔树脂纯化瓜馥木总黄酮,并评价其抗抑郁活性。以大孔树脂AB-8、HPD-600、HPD-826、X-5和D4006对瓜馥木总黄酮的吸附率和解吸率为指标筛选树脂种类,并对优选树脂的吸附特性和各影响因素进行研究,优化工艺条件。利用小鼠强迫游泳、小鼠悬尾和小鼠开野实验对瓜馥木总黄酮的抗抑郁活性进行了评价。结果表明AB-8具有较好的吸附率和解吸率,最佳纯化工艺为:上样流速为1.0 m L/min,树脂床用7 BV 5%乙醇除杂,再用8 BV 50%乙醇洗脱,洗脱流速1.0 m L/min,经AB-8大孔树脂纯化1次后,瓜馥木总黄酮含量为57.3%。瓜馥木总黄酮100 mg/kg剂量给药组能明显缩短小鼠强迫游泳和悬尾的不动时间,且该给药组小鼠的自主运动情况与阳性对照和空白之间没有显著差异。AB-8大孔树脂能较好地用于瓜馥木总黄酮的纯化,瓜馥木总黄酮具有明确的抗抑郁活性。
文摘AIM: To search some compounds that have anti-depressive activity from Helicid WN7-6 and its derivatives, and to explore their structure-function relationship. METHODS: 21 compounds including WN7-6, the lead compound were screened for antidepressant effect by the behavioral despair models(tail suspension test and the forced swimming test). And to study their stimulative effect on central nerve system(CNS) by mice autonomic activity test. RESULTS: Results shown that 11 of these compounds could decrease immobile time in suspend tail test and 6 compounds had the similar effect in force swimming test, compared with that of control group, the immobile time was obviously reduced (P<0.05, P<0.01). The results of autonomic activity test indicated that only 7 compounds could inhibit the activities of CNS (P<0.05, P<0.01). CONCLUSION: 30%-50% of these derivatives have anti-depressive activities, and much attention should be paid to further researches.
