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硫芥对大鼠淋巴细胞谷胱甘肽含量的影响 被引量:4
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作者 曾惠 刘勇 +2 位作者 朱明学 张黎明 董兆君 《第三军医大学学报》 CAS CSCD 北大核心 2004年第11期940-942,共3页
目的 探讨硫芥中毒后淋巴细胞谷胱甘肽含量变化。方法 用密度梯度离心法分离大鼠脾淋巴细胞 ,与硫芥共同进行体外培养。用MTT法测定细胞活力。以荧光分光光度计法测定细胞还原型谷胱甘肽 (glutathione ,GSH)、氧化型谷胱甘肽 (oxidize... 目的 探讨硫芥中毒后淋巴细胞谷胱甘肽含量变化。方法 用密度梯度离心法分离大鼠脾淋巴细胞 ,与硫芥共同进行体外培养。用MTT法测定细胞活力。以荧光分光光度计法测定细胞还原型谷胱甘肽 (glutathione ,GSH)、氧化型谷胱甘肽 (oxidizedglutathione ,GSSG)含量变化 ,结果 淋巴细胞活力在硫芥中毒后降低水平与细胞内GSH含量呈直线相关关系。硫芥中毒 8hGSH含量开始下降 ,8~ 12h维持在低水平 ,2 4~ 48hGSH进一步下降 ;GSSG含量 2 4~ 48h显著低于对照水平 ;GSH GSSG比值在 8h后显著降低。 展开更多
关键词 硫芥 还原性 抗氧化型谷胱甘肽 淋巴细胞
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The Effect of Herb Feeding on Antioxidant Liver Activity
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作者 Eliska Kabourkova Martina Lichovnikova Vojtech Adam 《Journal of Environmental Science and Engineering(B)》 2013年第10期602-607,共6页
The aim of the study was to evaluate the effect of 1.5% herb supplement of RL (rosemary leaves), YB (yarrow blooms), PL (plantain leaves), OS (oreganos talks) or red GP (grape pomace) on the broiler liver an... The aim of the study was to evaluate the effect of 1.5% herb supplement of RL (rosemary leaves), YB (yarrow blooms), PL (plantain leaves), OS (oreganos talks) or red GP (grape pomace) on the broiler liver antioxidant activity. Samples were analyzed by FRAP (ferric-reducing antioxidant power), FRK (free radicals) and DPPH (2,2-diphenyl-l-picrylhydrazyl) methods. Oxidative stress values (metallo thionein, reduced glutathione, oxidized glutathione and reduced/oxidized glutation ration) were measured in the blood and liver. Biochemical parameters (serum albumin, uric acid and bilirubin) were monitored in the blood. The antioxidant activity measured by the FRK method was higher in the oregano supplement (P 〈 0.05) than in plantain and rosemary supplements. 展开更多
关键词 FRAP FRK DPPH antioxidant activity herb.
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