Isothiocyanates(ITCs) extracts were prepared from fresh broccoli.Their antioxidant properties were evaluated by using the in vitro bioassays,including superoxide anion radical( 2 O·-) ,hydroxyl radical(HO·-)...Isothiocyanates(ITCs) extracts were prepared from fresh broccoli.Their antioxidant properties were evaluated by using the in vitro bioassays,including superoxide anion radical( 2 O·-) ,hydroxyl radical(HO·-) and 2,2-diphenyl-1-picrylhydracyl(DPPH·) radical-scavenging methods,lipid peroxidation assay,and reducing power assay.The ITCs extracts exhibited significant dose-dependent antioxidant activities(P<0.01) .Its antioxidant-stability was affected by temperature and storage condition,although it was more stable than vitamin C(Vc) .GC-MS iden-tified sulforaphane in the ITCs extracts.The antioxidant activity of sulforaphane was approximately one tenth to one fifth of that of Vc.Sulforaphane was experimented to be one of dominating contributors to antioxidant capacity of ITCs extracts.However,there was no direct antioxidant activity found for benzyl isothiocyanate(BITC) in vitro. Therefore,it could be concluded that the general group(-N=C=S) was not the essential part for the antioxidant activity of sulforaphane.It is suggested that the ITCs extracts from broccoli can be developed into a promising food additive for healthy food.展开更多
AIM： To the look at the current strength of evidence and the potential application of anti-oxidants in this setting.METHODS： Two electronic databases （PubMed and Web of Knowledge） were searched to January 2013 to ...AIM： To the look at the current strength of evidence and the potential application of anti-oxidants in this setting.METHODS： Two electronic databases （PubMed and Web of Knowledge） were searched to January 2013 to find studies addressing serum bilirubin levels in non-alcoholic fatty liver disease （NAFLD）. The search used key word combinations in relation to NAFLD and serum bilirubin specifc to human adults only. After screening selected studies were reviewed in depth by two inde-pendent reviewers. Data synthesis with further metaanalysis was planned but not possible due to the het-erogeneity of the outcome measures in these studies.RESULTS： Out of 416 studies screened only seven studies were considered suitable for inclusion. All seven studies consistently reported an inverse association of bilirubin with NAFLD despite the heterogeneous sample of studies. Only two studies were prospective. No negative studies were found.CONCLUSION： Most studies suggest a correlation between high bilirubin levels of any type are inversely correlated with NAFLD. But to date most of these stud-ies have been poorly designed to allow meaningful con-clusions, except one cohort study. There is a need for a large prospective cohort study in multiple populations to test this hypothesis fully before mechanistic associa-tions can be established and therapeutic options of the apparent anti-oxidant effect of bilirubin be explored in NAFLD. Furthermore these studies should include analysis of UGT1A1 gene to expound upon underlying cause of unconjugated hyperbilirubinaemia.展开更多
The possibilities of changes of ORP （oxidation-reduction water potential） with the help of chemical and physical-chemical methods were explored. Distillation and membrane technology were used as physical-chemical me...The possibilities of changes of ORP （oxidation-reduction water potential） with the help of chemical and physical-chemical methods were explored. Distillation and membrane technology were used as physical-chemical methods. In the case of application of chemical methods well-soluble substances were added into water. It was ascertained that the application of membrane technology makes it possible to obtain antioxidant water with negative ORR. Different energy change in a time unit can be applied in a whole number of technological processes and reveals new possibilities for many branches of industry.展开更多
Experimental results show that, all the sample of seasonal-temperature combinations, the effective component content is highest in November to collect leaves in the 25%; drying, and the strongest antioxidant activity....Experimental results show that, all the sample of seasonal-temperature combinations, the effective component content is highest in November to collect leaves in the 25%; drying, and the strongest antioxidant activity. The results of this study will provide theoretical basis for the preparation process of pomegranate leaf tea. Results of the correlation between antioxidant activity and the effective components show that the antioxidant activity of pomegranate leaves is closely related to polyphenol content, in particular had significant correlation with the content of tannin polyphenols, flavonoids, and ellagic acid content had no significant correlation.展开更多
OBJECTIVE: To assess the phytochemical contents and antioxidant activities of crude extracts from selected Tulbaghia species.METHODS: Standard methods were used for preliminary phytochemical analysis. The total phenol...OBJECTIVE: To assess the phytochemical contents and antioxidant activities of crude extracts from selected Tulbaghia species.METHODS: Standard methods were used for preliminary phytochemical analysis. The total phenolic acid contents of the plant extracts were determined using the Folin-Ciocalteu method, and the total flavonoid contents were determined using the aluminum chloride colorimetric method. 1,1-Diphenyl-2-picrylhydrazyl and 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) assays were used to evaluate the antioxidant activities.RESULTS: Phytochemical screening showed flavonoids, glycosides, tannins, terpenoids, saponins,and steroids were present in the Tulbaghia species.The total phenolic acid and flavonoid contents varied in the different plant extracts, ranging from4.50 to 11.10 mg of gallic acid equivalents per gram of fresh material and 3.04 to 9.65 mg of quercetin equivalents per gram, respectively. The IC50 values determined for Tulbaghia alliacea and Tulbaghia violacea based on 1,1-diphenyl-2-picrylhydrazyl(0.06 and 0.08 mg/m L, respectively) and 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)(0.06 and 0.03 mg/m L, respectively) were low and showed they had potential antioxidant activities.CONCLUSION: Our results suggest that individual compounds from Tulbaghia species should be isolated for analysis of their antioxidant activity because some compounds may work best when pure.展开更多
基金Supported by the Natural Science Foundation of Zhejiang Province(Y3090135)
文摘Isothiocyanates(ITCs) extracts were prepared from fresh broccoli.Their antioxidant properties were evaluated by using the in vitro bioassays,including superoxide anion radical( 2 O·-) ,hydroxyl radical(HO·-) and 2,2-diphenyl-1-picrylhydracyl(DPPH·) radical-scavenging methods,lipid peroxidation assay,and reducing power assay.The ITCs extracts exhibited significant dose-dependent antioxidant activities(P<0.01) .Its antioxidant-stability was affected by temperature and storage condition,although it was more stable than vitamin C(Vc) .GC-MS iden-tified sulforaphane in the ITCs extracts.The antioxidant activity of sulforaphane was approximately one tenth to one fifth of that of Vc.Sulforaphane was experimented to be one of dominating contributors to antioxidant capacity of ITCs extracts.However,there was no direct antioxidant activity found for benzyl isothiocyanate(BITC) in vitro. Therefore,it could be concluded that the general group(-N=C=S) was not the essential part for the antioxidant activity of sulforaphane.It is suggested that the ITCs extracts from broccoli can be developed into a promising food additive for healthy food.
文摘AIM： To the look at the current strength of evidence and the potential application of anti-oxidants in this setting.METHODS： Two electronic databases （PubMed and Web of Knowledge） were searched to January 2013 to find studies addressing serum bilirubin levels in non-alcoholic fatty liver disease （NAFLD）. The search used key word combinations in relation to NAFLD and serum bilirubin specifc to human adults only. After screening selected studies were reviewed in depth by two inde-pendent reviewers. Data synthesis with further metaanalysis was planned but not possible due to the het-erogeneity of the outcome measures in these studies.RESULTS： Out of 416 studies screened only seven studies were considered suitable for inclusion. All seven studies consistently reported an inverse association of bilirubin with NAFLD despite the heterogeneous sample of studies. Only two studies were prospective. No negative studies were found.CONCLUSION： Most studies suggest a correlation between high bilirubin levels of any type are inversely correlated with NAFLD. But to date most of these stud-ies have been poorly designed to allow meaningful con-clusions, except one cohort study. There is a need for a large prospective cohort study in multiple populations to test this hypothesis fully before mechanistic associa-tions can be established and therapeutic options of the apparent anti-oxidant effect of bilirubin be explored in NAFLD. Furthermore these studies should include analysis of UGT1A1 gene to expound upon underlying cause of unconjugated hyperbilirubinaemia.
文摘The possibilities of changes of ORP （oxidation-reduction water potential） with the help of chemical and physical-chemical methods were explored. Distillation and membrane technology were used as physical-chemical methods. In the case of application of chemical methods well-soluble substances were added into water. It was ascertained that the application of membrane technology makes it possible to obtain antioxidant water with negative ORR. Different energy change in a time unit can be applied in a whole number of technological processes and reveals new possibilities for many branches of industry.
文摘Experimental results show that, all the sample of seasonal-temperature combinations, the effective component content is highest in November to collect leaves in the 25%; drying, and the strongest antioxidant activity. The results of this study will provide theoretical basis for the preparation process of pomegranate leaf tea. Results of the correlation between antioxidant activity and the effective components show that the antioxidant activity of pomegranate leaves is closely related to polyphenol content, in particular had significant correlation with the content of tannin polyphenols, flavonoids, and ellagic acid content had no significant correlation.
基金Supported by Vaal University of Technology,Private Bag X021,Vanderbijlpark 1900,South Africa
文摘OBJECTIVE: To assess the phytochemical contents and antioxidant activities of crude extracts from selected Tulbaghia species.METHODS: Standard methods were used for preliminary phytochemical analysis. The total phenolic acid contents of the plant extracts were determined using the Folin-Ciocalteu method, and the total flavonoid contents were determined using the aluminum chloride colorimetric method. 1,1-Diphenyl-2-picrylhydrazyl and 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) assays were used to evaluate the antioxidant activities.RESULTS: Phytochemical screening showed flavonoids, glycosides, tannins, terpenoids, saponins,and steroids were present in the Tulbaghia species.The total phenolic acid and flavonoid contents varied in the different plant extracts, ranging from4.50 to 11.10 mg of gallic acid equivalents per gram of fresh material and 3.04 to 9.65 mg of quercetin equivalents per gram, respectively. The IC50 values determined for Tulbaghia alliacea and Tulbaghia violacea based on 1,1-diphenyl-2-picrylhydrazyl(0.06 and 0.08 mg/m L, respectively) and 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)(0.06 and 0.03 mg/m L, respectively) were low and showed they had potential antioxidant activities.CONCLUSION: Our results suggest that individual compounds from Tulbaghia species should be isolated for analysis of their antioxidant activity because some compounds may work best when pure.
