期刊文献+
共找到7篇文章
< 1 >
每页显示 20 50 100
紫花芸豆肽修复H2O2对HepG2细胞的氧化应激损伤 被引量:19
1
作者 马萍 程天赋 +3 位作者 郭增旺 周义 王欣 曹冬梅 《食品科学》 EI CAS CSCD 北大核心 2020年第1期175-182,共8页
目的:研究紫花芸豆肽(Phaseolus vulgaris peptides,PVPs)对H2O2诱导的HepG2细胞氧化损伤的保护作用及其作用机制。方法:体外培养HepG2细胞,实验分为空白组、模型组(正常培养24 h后加2 mmol/L H2O2刺激1 h)以及PVPs低、中、高剂量组(分... 目的:研究紫花芸豆肽(Phaseolus vulgaris peptides,PVPs)对H2O2诱导的HepG2细胞氧化损伤的保护作用及其作用机制。方法:体外培养HepG2细胞,实验分为空白组、模型组(正常培养24 h后加2 mmol/L H2O2刺激1 h)以及PVPs低、中、高剂量组(分别用50、100、200μg/mL PVPs处理24 h后,加2 mmol/L H2O2刺激1 h),采用水溶性四唑盐法(WST-1法)检测细胞增殖率,流式细胞仪检测胞内活性氧(reactive oxygen species,ROS)水平,酶联免疫吸附测定法检测胞内抗氧化物酶系活力,Western blot法检测凋亡蛋白表达量。结果:PVPs能缓解H2O2导致的HepG2细胞生长抑制,降低胞内ROS、丙二醛水平,提高超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶活力,并且下调p53、Caspase-3蛋白表达量。结论:PVPs对H2O2引起的HepG2细胞氧化损伤具有一定的保护作用,能抑制凋亡蛋白的表达,同时可以调节细胞的氧化还原系统、清除胞内ROS、提高胞内抗氧化物酶系的活力。 展开更多
关键词 紫花芸豆肽 氧化应激 抗氧化物酶系 凋亡蛋白
下载PDF
小米糠黄酮对H2O2致HepG2氧化应激损伤的保护作用 被引量:10
2
作者 郭增旺 樊乃境 +5 位作者 田海芝 滕飞 李萌 李杨 王中江 江连洲 《食品科学》 EI CAS CSCD 北大核心 2020年第5期159-165,共7页
目的:研究小米糠黄酮对H2O2致HepG2细胞氧化损伤的修复作用及其机制。方法:实验分为正常组、氧化应激模型组以及小米糠黄酮低、中、高剂量组,测定小米糠黄酮对H2O2诱导构建氧化应激损伤模型的细胞增殖率、胞内活性氧水平、抗氧化物酶系... 目的:研究小米糠黄酮对H2O2致HepG2细胞氧化损伤的修复作用及其机制。方法:实验分为正常组、氧化应激模型组以及小米糠黄酮低、中、高剂量组,测定小米糠黄酮对H2O2诱导构建氧化应激损伤模型的细胞增殖率、胞内活性氧水平、抗氧化物酶系和凋亡蛋白表达量的影响。结果表明:小米糠黄酮显著缓解H2O2对HepG2造成的细胞生长抑制(P<0.05),显著降低胞内活性氧和丙二醛的水平(P<0.05),显著提高超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶活力(P<0.05),并且显著下调bax、p53、Caspase-3凋亡蛋白的表达量(P<0.05)。小米糠黄酮对H2O2致HepG2氧化应激损伤具有保护作用,其作用机制可能和抑制细胞凋亡通路蛋白的表达,调控细胞的氧化还原系统、清除胞内活性氧水平、提高胞内抗氧化酶系的活性有关。 展开更多
关键词 小米糠黄酮 氧化应激 抗氧化物酶系 凋亡通路
下载PDF
The influence of cadmium on the antioxidant enzyme activities in polychaete Perinereis aibuhitensis Grube(Annelida:Polychaeta) 被引量:8
3
作者 袁秀堂 陈爱华 +2 位作者 周一兵 刘海映 杨大佐 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2010年第4期849-855,共7页
The infaunal polychaete Perinereis aibuhitensis Grube,distributed widely along Asian coasts and estuaries,is considered a useful animal model in ecotoxicological tests and a promising candidate in biomonitoring progra... The infaunal polychaete Perinereis aibuhitensis Grube,distributed widely along Asian coasts and estuaries,is considered a useful animal model in ecotoxicological tests and a promising candidate in biomonitoring programs.This paper deals with the activities of antioxidant enzymes including superoxide dismutase(SOD),catalase(CAT),and glutathione peroxidases(GSH-Px) in infaunal polychaete P.aibuhitensis exposed to a series of sublethal water-bound cadmium(Cd) concentrations(0,0.34,1.72,3.44,6.89,and 17.22 mg L-1) under a short-term exposure(1-8 d).The results indicate that the SOD and GSH-Px activities in P.aibuhitensis are stimulated first and then renewed to the original level.The CAT activity of worms decreases at an earlier exposure time but increases to the control values at a later exposure time.Our study suggests that Cd can interfere with the antioxidant defense system of P.aibuhitensis.However,the changes in antioxidant enzyme activities for this species do not show the best promise as biomarkers in Cd biomonitoring of estuarine and coastal zones because weak or non-dose-effect relationships between the antioxidant enzymes activities and Cd levels are found. 展开更多
关键词 Polychaete(Perinereis aibuhitensis) CADMIUM antioxidant enzyme biomarker biomonitoring.
下载PDF
Effects of UV-B irradiation on isoforms of antioxidant enzymes and their activities in red alga Grateloupia filicina(Rhodophyta) 被引量:3
4
作者 赵吉强 李丽霞 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2014年第6期1364-1372,共9页
Macroalgae in a littoral zone are inevitably exposed to UV-B irradiance. We analyzed the effects of UV-B on isoenzyme patterns and activities of superoxide dismutase (SOD), peroxidase (POX), catalase (CAT), and ... Macroalgae in a littoral zone are inevitably exposed to UV-B irradiance. We analyzed the effects of UV-B on isoenzyme patterns and activities of superoxide dismutase (SOD), peroxidase (POX), catalase (CAT), and ascorbate peroxidase (APX) of red algae Grateloupia filicina (Lamour.) C. Agardh. The activities of SOD, CAT, and APX changed in response to UV-B in a time- and dose-dependent manner. POX activity increased significantly under all three UV-B treatments. The enzymatic assay showed three distinct bands of SODI (Mn-SOD), SODII (Fe-SOD), and SODIII (CuZn-SOD) under a low (Luv) and medium (Muv) dose of UV-B irradiation, while SODI and SODIII activities decreased significantly when exposed to a high dose of UV-B irradiation (Huv). The activity of POX isoenzymes increased significantly after exposure to UV-B, which is consistent with the total activity. In addition, a clear decrease in activity of CATIV was detected in response to all the three doses of UV treatments. Some bands ofAPX isoenzyme were also clearly influenced by UV-B irradiation. Correspondingly, the daily growth rate declined under all the three exposure doses, and was especially significant under Muv and Huv treatments. These data suggest that, although the protection mechanisms of antioxidant defense system are partly inducible by UV-B to prevent the damage, G.filicina has incomplete tolerance to higher UV-B irradiation stress. 展开更多
关键词 UV-B irradiation Grateloupiafilicina antioxidant system ISOENZYME ELECTROPHORESIS
下载PDF
Response of enzymatic and non-enzymatic antioxidant defense systems of Polygonum hydropiper to Mn stress 被引量:2
5
作者 杨贤均 邓冬梅 +1 位作者 刘可慧 于方明 《Journal of Central South University》 SCIE EI CAS CSCD 2016年第4期793-797,共5页
The response of enzyme and non-enzymatic antioxidants of Mn hyperaccumuator, Polygonum hydropiper (P. hydropiper), to Mn stress was studied using hydroponics culture experiments to explore the mechanism of Mn tolera... The response of enzyme and non-enzymatic antioxidants of Mn hyperaccumuator, Polygonum hydropiper (P. hydropiper), to Mn stress was studied using hydroponics culture experiments to explore the mechanism of Mn tolerance in this species. Results showed that both chlorophyll and carotenoid contents significantly (p〈0.05) decreased with increasing Mn treatment levels (0, 0.5, 1, 2, 4, and 8 mg/L) in hydroponics. The concentrations of malondialdehyde (MDA) and hydrogen peroxide (H202) in the root and shoot of P hydropiper were accumulated under Mn stress. Meanwhile, the anti-oxidative functions of several important enzymes, including superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and peroxidase (POD) in plants were stimulated by Mn spike in leaves and roots, especially at low Mn stress; while sulfhydryl group (--SH) and glutathion (GSH) were likely involved in Mn detoxification ofP. hydropiper under high Mn stress. 展开更多
关键词 Polygonum hydropiper HYPERACCUMULATION enzymatic antioxidative defense non-enzymatic antioxidative defense
下载PDF
Influence of Irrigation with Reclaimed Water on Antioxidant System of Maize and Soybean
6
作者 Zhanbin Huang Wenying Li +4 位作者 Xiaoqing Wang Zhihua Jiao Yang Cao Shengpeng Jing Zhanxia Miao 《Journal of Life Sciences》 2010年第1期32-37,共6页
Irrigation with reclaimed water is an important way to deal with water shortage of agricultural production and solve the problem of sewage pollution. The response of crop antioxidant enzyme system is an early-warning ... Irrigation with reclaimed water is an important way to deal with water shortage of agricultural production and solve the problem of sewage pollution. The response of crop antioxidant enzyme system is an early-warning indicator of environmental factors changes. Compared with raw wastewater and used fresh water as controls, this research studied peroxides (POD), superoxide dismutase (SOD) activities and malondialdehyde (MDA) content in leaves from maize (Zea mays L.) and soybean (Phaseolus vulgaris L.) with pot culture under irrigating with reclaimed water which with secondary and third treatment. The results showed that secondary reclaimed water had some adverse effects on antioxidant system of maize in seedling stage, and the influence decreased in later stage of maize; effect of the third reclaimed water on antioxidant system of maize was not significant. Irrigation with reclaimed water has little influence on antioxidant system of soybean in seedling and reaping stage, but it could bring some oxidative stress in blossom stage. As a result, irrigating maize with second or third reclaimed water is safe to the growth of maize and soybean, but it is suggested that second reclaimed water should not be used in seedling stage of maize and reclaimed water should not be used in the second stage of soybean. 展开更多
关键词 Reclaimed water MAIZE SOYBEAN peroxidase (POD) superoxide dismutase (SOD) malondialdehyde (MDA).
下载PDF
Antioxidant effect of indirect moxibustion on healthy subjects:a pilot study 被引量:7
7
作者 Hyejung Park Hyeunggeug Kim +2 位作者 Sara Yoo Namhun Lee Changgue Son 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2012年第4期590-595,共6页
OBJECTIVE:Moxibustion has long been thought to promote vital energy or immunity through clinical experience.This study aimed to investigate the clinical effects of indirect moxibustion on antioxidant and immunomodulat... OBJECTIVE:Moxibustion has long been thought to promote vital energy or immunity through clinical experience.This study aimed to investigate the clinical effects of indirect moxibustion on antioxidant and immunomodulation in a normal population.METHODS:Twenty-five healthy volunteers with no objective or subjective disorders were recruited.The participants were treated with indirect moxibustion on acupoints Guanyuan(CV 4) and Shenque(CV 8) three times per week for 4 weeks.The serum levels of reactive oxygen species(ROS) and malondialdehyde(MDA),the total antioxidant capacity(TAC),the activities of catalase and superoxide dismutase(SOD),and the total glutathione content were determined before and after the 12th moxibustion.Lymphocyte subpopulations and 42 cytokines in the peripheral blood were analyzed using flow cytometry and antibody array,respectively.RESULTS:Compared with the initial time point,the serum levels of ROS and MDA were significantly lowered by moxibustion,while TAC was increased(P<0.01 for all).A significant increase was observed in catalase activity(P<0.05),but not in SOD or total glutathione.There were no significant changes in lymphocyte subpopulations or cytokines in the peripheral blood.Fifteen of 25 participants reported at least one symptom in which they felt subjective improvement after moxibustion.CONCLUSION:Indirect moxibustion on acupoints CV 4 and CV 8 improved the antioxidant defense system,which may be a mechanism explaining the clinical effects of moxibustion. 展开更多
关键词 MOXIBUSTION Point CV 4 (Guanyuan) Point CV 8 (Shenque) ANTIOXIDANTS Pilot projects
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部