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青萝卜汁提取物及肝特灵抗肝细胞脂质过氧化损伤作用研究 被引量:6
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作者 高斌 袁桂玉 +3 位作者 梁树仁 奚明 曹武奎 李丽芬 《天津中医》 2000年第4期8-10,共3页
关键词 青萝卜汁提取物 特灵 细胞脂质过氧化损伤
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齐墩果烷及熊果烷型五环三萜抗肝损伤作用及其构效关系研究 被引量:5
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作者 宁娱 张润 +5 位作者 邹子军 孙洋 陈杰桃 林朝展 熊天琴 祝晨蔯 《天然产物研究与开发》 CAS CSCD 北大核心 2013年第10期1346-1351,共6页
研究3种广东地区习用中药中32个齐墩果烷及熊果烷型五环三萜化合物对肝损伤的保护作用及其构效关系。采用体外氧化肝损伤模型,测定分离得到的五环三萜化合物对损伤肝细胞的保护作用;以化合物的分子结构参数与损伤肝细胞保护作用构建比... 研究3种广东地区习用中药中32个齐墩果烷及熊果烷型五环三萜化合物对肝损伤的保护作用及其构效关系。采用体外氧化肝损伤模型,测定分离得到的五环三萜化合物对损伤肝细胞的保护作用;以化合物的分子结构参数与损伤肝细胞保护作用构建比较分子场分析(CoMFA)模型,并研究其构效关系。建立的CoMFA模型证实上述化合物的结构参数与其对氧化肝损伤细胞保护活性存在明显的相关性(模型的相关系数γ2为0.976),且有良好的预测能力(交叉验证相关系数q2为0.719),同时使用"留一法"证实模型的稳定性和可靠性。熊果烷型三萜较齐敦果烷型三萜的保肝活性明显增强,根据获得的模型的三维等势图,前者在3、19、20、23和24等5个取代位置添加带负电荷的基团,如-OH,则使化合物的保肝活性明显提高;如在这些位置引入疏水性较强的基团,如-CH3等,则可能使化合物的保肝活性降低。应用所构建的CoMFA模型预测化合物的活性与实测值接近,表明所建模型具有良好的预测性。 展开更多
关键词 抗氧化肝损伤 五环三萜类 定量构效关系 比较分子场
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Protective effect of some vitamins against the toxic action of ethanol on liver regeneration induced by partial hepatectomy in rats 被引量:8
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作者 Carlett Ramírez-Farías Eduardo Madrigal-Santillán +6 位作者 José Gutiérrez-Salinas Nidia Rodríguez-Sánchez Maricela Martínez-Cruz Ilse Valle-Jones Ingrid Gramlich-Martínez Alejandra Hernández-Ceruelos José A Morales-González 《World Journal of Gastroenterology》 SCIE CAS CSCD 2008年第6期899-907,共9页
AIM: To investigate the effects of vitamins (A, C and E) on liver injury induced by ethanol administration during liver regeneration in rats. METHODS: Male Wistar rats subjected to 70% partial hepatectomy were divided... AIM: To investigate the effects of vitamins (A, C and E) on liver injury induced by ethanol administration during liver regeneration in rats. METHODS: Male Wistar rats subjected to 70% partial hepatectomy were divided into five groups (groups 1-5). During the experiment, animals of Group 1 drank only water. The other four groups (2-5) drank 30 mL of ethanol/L of water. Group 3 additionally received vitamin A, those of group 4 vitamin C and those of group 5 received vitamin E. Subsequently serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), albumin and bilirubin were measured colorimetrically. Lipid peroxidation (thiobarbituric-acid reactive substances, TBARS) both in plasma and liver was measured, as well as liver mass gain assessment and total DNA. RESULTS: Compared with sham group, serum AST and ALT increased significantly under ethanol treatment (43% and 93%, respectively, with P < 0.05). Vitamin C and vitamin E treatment attenuated the ethanol-induced increases in ALT and AST activity. Ethanol treatment also decreased serum albumin concentration compared to sham group (3.1 ± 0.4 g/dL vs 4.5 ± 0.2 g/dL; P < 0.05). During liver regeneration vitamins C and E significantly ameliorated liver injury for ethanol administration in hepatic lipid peroxidation (4.92 nmol/mg and 4.25 nmol/mg vs 14.78 nmol/mg, respectively, with P < 0.05). In association with hepatic injury, ethanol administration caused a significant increase in both hepatic and plasma lipid peroxidation. Vitamins (C and E) treatment attenuated hepatic and plasma lipid peroxidation. CONCLUSION: Vitamins C and E protect against liver injury and dysfunction, attenuate lipid peroxidation, and thus appear to be significantly more effective than vitamin A against ethanol-mediated toxic effects during liver regeneration. 展开更多
关键词 Lipid peroxidation Antioxidant vitamins Alcohol-induced liver injury Liver regeneration
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Reduced antioxidant level and increased oxidative damage in intact liver lobes during ischaemia-reperfusion 被引量:6
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作者 LászlóVáli Gabriella Taba +5 位作者 KláraSzentmihályi HedvigFébel TímeaKurucz Zsolt Pallai PéterKupcsulik AnnaBlázovics 《World Journal of Gastroenterology》 SCIE CAS CSCD 2006年第7期1086-1091,共6页
AIM: To determine whether increased blood flow of the liver can cause oxidative stress and hepatocyte damage, and to elaborate methods suitable for measuring the antioxidant defence during hepatic surgery on rat mode... AIM: To determine whether increased blood flow of the liver can cause oxidative stress and hepatocyte damage, and to elaborate methods suitable for measuring the antioxidant defence during hepatic surgery on rat model.METHODS: In nembutal narcosis, the left lateral and the medial lobes of the liver were clipped for 45 rain to make the total blood supply flow through the other lobes. Total antioxidant status, glutathione peroxidase and superoxide dysmutase activity, as well as the concentrations of diene conjugates and free sulphydril groups, H-donating ability and reducing power of the liver samples were determined. Chemiluminescent intensity of the liver was also measured. Metal ions (Al, Ca, Cu, Fe, Mg, Mn, Zn) and P and S concentrations of the liver were determined with an inductively coupled plasma optical emission spectrometer and Se content was measured by cathodic stripping voltammetry.RESULTS: Glutathione peroxidase and superoxide dysmutase activities of the liver decreased significantly in the hyperemia group compared to those observed in the sham operated group. The level of total antioxidant status was also significantly lower in the hyperemia group. H-donating ability, reducing power and free sulphydril group concentration showed the same tendency. A significant correlation (P〈0.05) was found between the changes in non-specific antioxidant activities. This pointed to simultaneous activity of the antioxidant defence system. Al, Cu, Mn, Zn, and S were lower in the hyperemia group than in the sham operated group when the levels of Ca, Fe, Mg, Se and P ions were higher during hyperemia.CONCLUSION: Oxidative stress is one of the main factors for the injury of intact liver lobes during ischaemia-reperfusion. 展开更多
关键词 Free radicals LIVER Oxidative stress Metal elements Redox-balance
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Estradiol-17β protects against hypoxia-induced hepatocyte injury through ER-mediated upregulation of Bcl-2 as well as ER-independent antioxidant effects 被引量:4
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作者 Min Young Lee Sun Chul Jung +1 位作者 Jang Hem Lee Ho Jae Han 《Cell Research》 SCIE CAS CSCD 2008年第4期491-499,共9页
Although many previous studies have suggested that estrogen functions as a cytoprotective agent under oxidative stress conditions, the underlying mechanism by which this effect is exerted remains to be elucidated. Thi... Although many previous studies have suggested that estrogen functions as a cytoprotective agent under oxidative stress conditions, the underlying mechanism by which this effect is exerted remains to be elucidated. This study assessed the effects of estradiol-17β (E2) (10^-8s M) on hypoxia-induced cell injury and its related signaling in primary cultured chicken hepatocytes. Hypoxic conditions were found to augment the level of DNA damage and to reduce cell viability and the level of [^3H]-thymidine incorporation, and these phenomena were prevented through treatment with E2. Hypoxia also increased caspase-3 expression, but showed no evidence of an influence on the expression of Bcl-2. However, E2 induced an increase in the level of Bcl-2 expression under hypoxic conditions and reduced the level of caspase-3 expression. The effects of E2 on Bcl-2 and caspase expression were blocked by ICI 182780 (E2 receptor (ER) antagonist, 10"7 M). In addition, hypoxia resulted in an increase in the intracellular reactive oxygen species (ROS) generated. These effects were blocked by E2, but not by E2-BSA and ICI 182780. Hypoxia also activated p38 mitogen-activated protein kinase (MAPK), c-JUN N-terminal kinase/stress-activated protein kinase (JNK/SAPK) and nuclear factor-kB (NF-kB). These effects were blocked by E2, but not by ICI 182780. The inhibition of p38 MAPK and JNK/SAPK blocked NF-kB activation. In conclusion, E2 was found to protect against hypoxia-induced cell injury in chicken hepatocytes through ER-mediated upregulation of Bcl-2 expression and through reducing the activity of ROS-dependent p38 MAPK, JNK/ SAPK and NF-kB. 展开更多
关键词 HYPOXIA estradiol-17β chicken hepatocytes
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Antioxidant and hepatoprotective effect of different extracts of Guizhencao (Herba Bidentis Bipinnatae) against liver injury in hyperlipidemia rats 被引量:1
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作者 Xichao Xia Yuhong Ma +5 位作者 Xiankun Xing Chuanfeng Huang Ling Li Gaixia Gui Qingchun Liu Shipeng Xue 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2013年第4期518-523,共6页
OBJEVTIVE: To investigate the antioxidant and hepatoprotective properties of the different extracts Guizhencao (Herba Bidentis Bipinnatae) against liver injury in hyperlipidemia rats. METHODS: The rats were divided in... OBJEVTIVE: To investigate the antioxidant and hepatoprotective properties of the different extracts Guizhencao (Herba Bidentis Bipinnatae) against liver injury in hyperlipidemia rats. METHODS: The rats were divided into 7 groups, with 10 rats in each. Rats were treated with high-fat diet for 18 weeks besides the normal control group, then rats in both normal control and model groups were received 5 mL/kg -1 · day -1 of saline and those in the positive control group with 2 mg/kg -1 · day -1 of lovastatin. Rats in the positive control group and different Guizhencao (Herba Bidentis Bipinnatae) extracts treatment groups (ethyl acetate extract group, n-hexane extract group, ethanol extract group, and aqueous extract group) were treated with corresponding extract at a concentration of 5 mL/kg -1 · day -1 . After 8 weeks treatment, all rats were sacrificed and total blood samples were collected. Histological analysis of liver was underdone by hematoxylin and eosin. The levels of serum ala-nine aminotransferase (ALT), aspartate aminotransferase (AST), glouse (GLU), cholesterol (TC), triglycerides (TG), low-density lipoprotein-cholesterol (LDL-C) and high-density lipoprotein-cholesterol were measured according to standard procedure using auto-analyzer. The superoxide dismutase (SOD) and malondialdehyde (MDA) levels in liver were ananlyzed by procedure instruction. RESULTS: The histopathological analysis implied that the administration of Guizhencao (HerbaBidentis Bipinnatae) extracts resulted in hepatoprotective role compared with that of the model group. In addition, the high-fat diet caused a remarkable increase of ALT, AST, GLU, TC, TG, LDL-C and MDA levels. A decline in HDL-C and SOD concentrations and a reversal of effects were observed in different Guizhencao (Herba Bidentis Bipinnatae) extracts groups, especially in the aqueous extract and ethanol extract groups. CONCLUSION: The different extracts of Guizhencao (Herba Bidentis Bipinnatae) can play a protecting role against liver injury in hyperlipidemia rats maybe through decreasing ALT, AST, GLU, TC, TG, LDL-C and MDA levels and enhancing the liver anti-oxidative ability. 展开更多
关键词 Bidens Antioxidants Drug-induced liver injury Hyperlipidemias
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