中国大豆种质抗SCN基因rhg1位点SSR标记等位变异特点分析

利用与抗大豆胞囊线虫病主效基因rhg1紧密连锁的SSR标记Satt30 9分析 6 34份中国大豆种质 ,以 2 1份美国种质为对照 ,目的是明确我国抗大豆胞囊线虫病种质资源的特点 ,为大豆抗胞囊线虫病种质资源鉴定及抗线育种提供依据。 14 9份免疫或高抗种质和 4 95份感病种质共鉴定出 6个等位变异 ,其中介于 134bp和 14 9bp之间的Satt30 9- 5为新等位变异 ,具有该等位变异的陕西三股条黑豆和山西忻县小黑豆分别免疫和中抗 1号生理小种。对不同等位变异、不同抗性等级和抗不同生理小种种质的分析表明 :92 .13%的感病种质具有 12 5bp、131bp或 14 9bp等位变异 ,抗病种质在各等位变异都有分布 ,但以 12 8bp和 134bp为主 ,在具有这两个等位变异的种质中 ,有 72 .4 6 %表现为抗病 ,且 77.2 7%的免疫种质、87.6 9%的双抗种质和 10 0 %的三抗种质都具有这两个等位变异。16份具有 12 8bp等位变异的种质中有 14份来自山西省 ,另有 2份是黑龙江省和河北省的育成品种 ,推测山西省可能是Satt30 9位点 12 8bp等位变异种质的起源地。本研究结果可为大豆抗胞囊线虫病育种的抗源选择提供一定的参考依据。

Constructing Biophore of Uroselective α1-Adrenoceptor antagonist

Aim The biophore of uroselective α_1-adrenoceptor antagonist was studied byusing Apex-3D software on an 02 Silicon Graphics Computer Station. Methods Five known antagonists(Indoramin, GG-818, RS100975, R-YM12167, and KMD-3213), which possess both good selectivity and highaffinities to prostate and α_1-AR subtype, were chosen for building the biophore. Using anautomatic filtering software for obtaining reasonable biophores, the filter parameters wereselected: P (probability) > 0.8, active (number of active compounds) ≥ 4, and size (descriptorcenter) ≥ 3. Results Three biophores conformed to the requirements, each of whom contained a basiccenter, an aromatic ring center and H-site according to the structure-activity relationships ofknown α_1-adrenoceptor antagonist. Conclusion The biophore model developed by computer simulationwith Apex-3D software can be used to design and synthesize a new α_1-adrenoceptor antagonist withhigh activity and low side effect.