Objectives Because the endothelium is a major target for inflammatory c ytokine s, we investigated whether elevated interleukin (IL)-10 serum levels are associ ated with improved endothelial vasoreactivity in patients...Objectives Because the endothelium is a major target for inflammatory c ytokine s, we investigated whether elevated interleukin (IL)-10 serum levels are associ ated with improved endothelial vasoreactivity in patients with coronary artery d isease (CAD). Background Chronic inflammation plays a pivotal role in the progre ssion of atherosclerosis. Interleukin-10 is an anti-inflammatory cytokine that exerts important protective effects on atherosclerotic lesion development in ex perimental animals. Methods Vasoreactivity was assessed in 65 male patients with documented CAD by measuring endothelium-dependent (acetylcholine <<ACh>> 10 to 5 0 μg/min) and endothelium-independent (sodium nitroprusside <<SNP>> 2 to 8 μg/m in) forearm blood flow (FBF) responses using venous occlusion plethysmography. R esults Serum levels of IL-10 were significantly correlated with ACh-induced FB F responses (r=0.31, p< 0.02), but not with SNP responses. Importantly, if IL-1 0 serum levels were increased in patients with elevated C-reactive protein (CRP ) levels, no impairment of ACh-stimulated FBF response was observed. On multiva riate analysis, including low-density lipoprotein cholesterol, smoking, hyperte nsion, diabetes, clinical status of the patients, and statin and/or angiotensin -converting enzyme inhibitor treatment, only IL-10 (p< 0.02) and CRP serum lev els (p< 0.02) were significant independent predictors of ACh-induced FBF respon ses. Conclusions Thus, increased IL-10 serum levels are associated with improve d systemic endothelial vasoreactivity in patients with elevated CRP serum levels , demonstrating that the balance between pro-and anti-inflammatory mediators i s a major determinant of endothelial function in patients with CAD.展开更多
Thais luteostoma has been utilized as a crude drug whose shell and soft tissue have been widely used for the treatment of heat syndrome in China for thousands of years. The present study was designed to investigate th...Thais luteostoma has been utilized as a crude drug whose shell and soft tissue have been widely used for the treatment of heat syndrome in China for thousands of years. The present study was designed to investigate the antipyretic and anti-inflammatory activities of T. luteostoma. T. luteostoma was divided into shell （TLSH） and soft tissue （TLST） samples in the present study. The rat model of yeast-induced fever was used to investigate their antipyretic effects; and the rat model of hind paw edema induced by carrageenan was utilized to study their anti-inflammatory activities, and at the same time, the concentration variations of the central neurotransmitter [prostaglandin E2 （PGE2） and cyclic adenosine monophosphate （cAMP）], inflammatory mediators [tumor necrosis factor （TNFα）, interleukin-1β （IL-1）, interleukin-2 （IL-2） and interleukin-6 （IL-6）] and ion （Na^＋ and Ca^2＋） were also tested. The results showed that TLSH and TLST extracts significantly inhibited yeast-induced pyrexia in rats （P 〈 0.05）, and exhibited more lasting effects as compared to aspirin, and TLSH had the better antipyretic activity than TLST, and that TLSH and TLST could significantly prevent against carrageenan induced paw edema in rats （P 〈 0.05）; and markedly reduced levels of PGE2, cAMP, TNFα, IL-1β, IL-2, IL-6, and Na^＋/Ca^2＋. In fever model, TLST could significantly reduce the levels of PGE2 （P 〈 0.01） in rats＇ homogenate and TNF a （P 〈 0.05）, IL-113 （P 〈 0.01） in the plasma than TLSH, whereas TLSH could reduce the content of IL-2 （P 〈 0.01） and IL-6 （P 〈 0.01） in plasma and increase the content of Ca2＋ （P 〈 0.01） in plasma and homogenate more significantly than TLST. In conclusion, T. luteostoma extract has antipyretie and anti-inflammatory activities, which may be mediated through the suppression of production of PGE2, cAMP, Na^＋/Ca^2＋ , TNF a, IL-1β, IL-2, and IL-6.展开更多
文摘Objectives Because the endothelium is a major target for inflammatory c ytokine s, we investigated whether elevated interleukin (IL)-10 serum levels are associ ated with improved endothelial vasoreactivity in patients with coronary artery d isease (CAD). Background Chronic inflammation plays a pivotal role in the progre ssion of atherosclerosis. Interleukin-10 is an anti-inflammatory cytokine that exerts important protective effects on atherosclerotic lesion development in ex perimental animals. Methods Vasoreactivity was assessed in 65 male patients with documented CAD by measuring endothelium-dependent (acetylcholine <<ACh>> 10 to 5 0 μg/min) and endothelium-independent (sodium nitroprusside <<SNP>> 2 to 8 μg/m in) forearm blood flow (FBF) responses using venous occlusion plethysmography. R esults Serum levels of IL-10 were significantly correlated with ACh-induced FB F responses (r=0.31, p< 0.02), but not with SNP responses. Importantly, if IL-1 0 serum levels were increased in patients with elevated C-reactive protein (CRP ) levels, no impairment of ACh-stimulated FBF response was observed. On multiva riate analysis, including low-density lipoprotein cholesterol, smoking, hyperte nsion, diabetes, clinical status of the patients, and statin and/or angiotensin -converting enzyme inhibitor treatment, only IL-10 (p< 0.02) and CRP serum lev els (p< 0.02) were significant independent predictors of ACh-induced FBF respon ses. Conclusions Thus, increased IL-10 serum levels are associated with improve d systemic endothelial vasoreactivity in patients with elevated CRP serum levels , demonstrating that the balance between pro-and anti-inflammatory mediators i s a major determinant of endothelial function in patients with CAD.
基金supported by Marine Industry Research Special Funds for Public Welfare Projects(201205024–1)The Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(ysxk-2010)
文摘Thais luteostoma has been utilized as a crude drug whose shell and soft tissue have been widely used for the treatment of heat syndrome in China for thousands of years. The present study was designed to investigate the antipyretic and anti-inflammatory activities of T. luteostoma. T. luteostoma was divided into shell （TLSH） and soft tissue （TLST） samples in the present study. The rat model of yeast-induced fever was used to investigate their antipyretic effects; and the rat model of hind paw edema induced by carrageenan was utilized to study their anti-inflammatory activities, and at the same time, the concentration variations of the central neurotransmitter [prostaglandin E2 （PGE2） and cyclic adenosine monophosphate （cAMP）], inflammatory mediators [tumor necrosis factor （TNFα）, interleukin-1β （IL-1）, interleukin-2 （IL-2） and interleukin-6 （IL-6）] and ion （Na^＋ and Ca^2＋） were also tested. The results showed that TLSH and TLST extracts significantly inhibited yeast-induced pyrexia in rats （P 〈 0.05）, and exhibited more lasting effects as compared to aspirin, and TLSH had the better antipyretic activity than TLST, and that TLSH and TLST could significantly prevent against carrageenan induced paw edema in rats （P 〈 0.05）; and markedly reduced levels of PGE2, cAMP, TNFα, IL-1β, IL-2, IL-6, and Na^＋/Ca^2＋. In fever model, TLST could significantly reduce the levels of PGE2 （P 〈 0.01） in rats＇ homogenate and TNF a （P 〈 0.05）, IL-113 （P 〈 0.01） in the plasma than TLSH, whereas TLSH could reduce the content of IL-2 （P 〈 0.01） and IL-6 （P 〈 0.01） in plasma and increase the content of Ca2＋ （P 〈 0.01） in plasma and homogenate more significantly than TLST. In conclusion, T. luteostoma extract has antipyretie and anti-inflammatory activities, which may be mediated through the suppression of production of PGE2, cAMP, Na^＋/Ca^2＋ , TNF a, IL-1β, IL-2, and IL-6.
