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系统性红斑狼疮患者抗热休克蛋白90抗体的检测 被引量:2
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作者 朱里 黄长征 +2 位作者 冯爱平 刘厚君 涂亚庭 《华中科技大学学报(医学版)》 CAS CSCD 北大核心 2009年第1期131-132,136,共3页
目的通过对系统性红斑狼疮(SLE)患者抗热休克蛋白90抗体(HSP90-Ab)的检测,探讨其在SLE发病中的作用。方法采用Western blot法检测45例SLE患者抗HSP90抗体的表达情况,并分析其与HSP90蛋白表达间的相关性。另取15例健康血样进行正常对照... 目的通过对系统性红斑狼疮(SLE)患者抗热休克蛋白90抗体(HSP90-Ab)的检测,探讨其在SLE发病中的作用。方法采用Western blot法检测45例SLE患者抗HSP90抗体的表达情况,并分析其与HSP90蛋白表达间的相关性。另取15例健康血样进行正常对照。结果45例SLE患者血浆中HSP90 IgG抗体阳性表达23例(51.11%),HSP90 Ig M抗体阳性表达12例(26.67%)。并且HSP90蛋白与HSP90 IgG抗体间呈显著的正相关关系(χ2=4.15,P<0.05)。15例正常对照组中未见HSP90 IgG/Ig M抗体阳性表达。结论SLE患者体内高表达的HSP90可诱导产生抗HSP90自身抗体,其在SLE的自身免疫损伤机制中可能发挥重要作用。 展开更多
关键词 红斑狼疮 系统性 抗热休克蛋白90抗体
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冠心病患者血浆中抗热休克蛋白70抗体表达水平的研究
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作者 杨杪 谭皓 +5 位作者 陈永文 王峰 何美安 蒋长征 陈胜 邬堂春 《劳动医学》 北大核心 2004年第2期139-140,148,共3页
[目的 ]检测冠心病患者血浆中抗热休克蛋白 70 (HSP70 )抗体水平与正常人有无差异 ,并探讨其与冠心病发生发展之间的关系。 [方法 ]采用Western ELISA方法检测 192例冠心病患者中抗HSP70抗体水平 ,并以 192例无冠心病的个体作为对照。[... [目的 ]检测冠心病患者血浆中抗热休克蛋白 70 (HSP70 )抗体水平与正常人有无差异 ,并探讨其与冠心病发生发展之间的关系。 [方法 ]采用Western ELISA方法检测 192例冠心病患者中抗HSP70抗体水平 ,并以 192例无冠心病的个体作为对照。[结果 ] 192例冠心病患者中抗HSP70抗体阳性 7例 ,对照组 5例 ,病例组抗体阳性率与对照组之间差异无显著性 (P >0 .0 5 )。 [结论 ]本研究未发现抗HSP70抗体与冠心病的发生发展有联系 ,抗HSP70抗体在冠心病发病过程中的作用有待于进一步探讨。 展开更多
关键词 冠心病 血浆 抗热休克蛋白70 抗体表达 HSP70 CHD 血管性疾病
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氟暴露工人血浆抗热休克蛋白70抗体表达水平的研究
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作者 徐增光 邓生琼 +3 位作者 杨江林 郭怀兰 王建洲 刘先玲 《数理医药学杂志》 2005年第5期421-422,共2页
目的:检测氟暴露工人血浆抗热休克蛋白70(HSP70)抗体表达情况,探讨其与氟暴露应激反应的关系。方法:选择某铝厂氟暴露工人和非氟暴露工人各22人,用氟离子选择电极法测定血清氟、尿氟离子浓度,用Western-ELISA方法检测血浆抗HSP70抗体阳... 目的:检测氟暴露工人血浆抗热休克蛋白70(HSP70)抗体表达情况,探讨其与氟暴露应激反应的关系。方法:选择某铝厂氟暴露工人和非氟暴露工人各22人,用氟离子选择电极法测定血清氟、尿氟离子浓度,用Western-ELISA方法检测血浆抗HSP70抗体阳性率。结果:氟暴露组工人血清氟、尿氟离子浓度明显高于非暴露组(P<0.01);血浆HSP70抗体阳性率比较,氟暴露组抗HSP70抗体阳性率明显高于非暴露组(P<0.01)。结论:氟暴露环境下工人抗HSP70抗体阳性率明显增加,可能与氟暴露环境的应激保护反应有关。 展开更多
关键词 抗热休克蛋白70抗体
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噪声作业工人血浆抗热休克蛋白70抗体的应用分析 被引量:2
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作者 胡家培 董明国 《中国职业医学》 CAS 北大核心 2007年第4期335-335,338,共2页
目的探讨噪声作业工人血浆中抗热休克蛋白70(HSP70)抗体水平及其与累计噪声暴露量(CNE)的关系。方法采用蛋白电泳-酶联免疫吸附实验法对本市某机械制造公司380名工人(噪声暴露200名,对照180名)的血浆中HSP70抗体水平进行测定。用趋势2... 目的探讨噪声作业工人血浆中抗热休克蛋白70(HSP70)抗体水平及其与累计噪声暴露量(CNE)的关系。方法采用蛋白电泳-酶联免疫吸附实验法对本市某机械制造公司380名工人(噪声暴露200名,对照180名)的血浆中HSP70抗体水平进行测定。用趋势2检验分析HSP70抗体水平与噪声暴露量的关系。结果噪声暴露组HSP70抗体阳性率(41.0%)明显高于对照组(6.6%)。HSP70抗体阳性率随CNE的增高而明显升高。结论HSP70抗体阳性与CNE有密切关系。 展开更多
关键词 累计噪声暴露量 抗热休克蛋白 抗体
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幽门螺杆菌阳性慢性胃炎、消化性溃疡与血清抗热休克蛋白60抗体的关系
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作者 严洁 占强 +5 位作者 朱晓红 夏敏 尤一萍 杜虎 张国强 郭继忠 《中华消化内镜杂志》 2003年第6期421-422,共2页
热休克蛋白(Hsp)是一种应激蛋白,在幽门螺杆菌(Hp)及人胃黏膜细胞中均有表达.研究表明Hp和宿主胃黏膜细胞所表达的Hsp具有一致的抗原结构,因此可能诱导宿主产生自身免疫反应,导致多种疾病[1,2].我们初步研究Hp感染的慢性胃炎、消化性溃... 热休克蛋白(Hsp)是一种应激蛋白,在幽门螺杆菌(Hp)及人胃黏膜细胞中均有表达.研究表明Hp和宿主胃黏膜细胞所表达的Hsp具有一致的抗原结构,因此可能诱导宿主产生自身免疫反应,导致多种疾病[1,2].我们初步研究Hp感染的慢性胃炎、消化性溃疡患者及其血清抗幽门螺杆菌Hsp60抗体表达之间的关系,报告如下. 展开更多
关键词 幽门螺杆菌阳性 慢性胃炎 消化性溃疡 血清抗热休克蛋白60抗体
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eHSP的研究进展 被引量:2
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作者 任碧琼 余平 《国际病理科学与临床杂志》 CAS 2011年第5期439-443,共5页
热休克蛋白(heat shock protein,HSP)一直被认为是细胞内蛋白质,在胞内通过帮助蛋白质正确折叠、组装以及促进变性蛋白质降解而发挥生物学功能。HSP能被主动地分泌到胞外,成为细胞外HSP(extracellularHSPe,HSP),但其分泌及作用机制尚未... 热休克蛋白(heat shock protein,HSP)一直被认为是细胞内蛋白质,在胞内通过帮助蛋白质正确折叠、组装以及促进变性蛋白质降解而发挥生物学功能。HSP能被主动地分泌到胞外,成为细胞外HSP(extracellularHSPe,HSP),但其分泌及作用机制尚未定论。HSP偶然释放到外周循环是通过细胞坏死,生理性释放则是通过活化分泌系统。HSP有一种重要的前炎症因子可通过相同的分泌系统分泌,抗HSP自身抗体可以促进HSP的细胞因子样、辅佐样活性,抗HSP自身抗体与HSP或者HSP受体交联是放大HSP免疫信号作用的可能机制。 展开更多
关键词 细胞外热休克蛋白 细胞因子 休克蛋白受体 抗热休克蛋白自身抗体
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Protective effect of curcumin against liver warm ischemia/reperfusion injury in rat model is associated with regulation of heat shock protein and antioxidant enzymes 被引量:34
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作者 Shi-Qiang Shen Yuan Zhang +1 位作者 Jin-Jian Xiang Cheng-Long Xiong 《World Journal of Gastroenterology》 SCIE CAS CSCD 2007年第13期1953-1961,共9页
AIM: To investigate the hypothesis that the protective effects of curcumin in hepatic warm ischemia/reperfusion (I/R) injury are associated with increasing heat shock protein 70 (Hsp70) expression and antioxidant... AIM: To investigate the hypothesis that the protective effects of curcumin in hepatic warm ischemia/reperfusion (I/R) injury are associated with increasing heat shock protein 70 (Hsp70) expression and antioxidant enzyme activity. METHODS: Sixty Sprague-Dawley male rats were randomly divided into sham, I/R, C + I/R groups. The model of reduced-size liver warm ischemia and reperfusion was used. Curcumin (50 mg/kg) was administered by injection through a branch of superior mesenteric vein at 30 min before ischemia in C + I/R group. Five rats were used to investigate the survival during 1 wk after operation in each group. Blood samples and liver tissues were obtained in the remaining animals after 3, 12, and 24 h of reperfusion to assess serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), liver tissue NO2- + NO3-, malondialdehyde (MDA) content, superoxide dismutase (SOD), catalase (CAT), nitricoxide synthase (NOS) and myeloperoxidase (MPO) activity, HspT0 expression and apoptosis ratio. RESULTS: Compared with I/R group, curcumin pretreatment group showed less ischemia/reperfusioninduced injury. CAT and SOD activity and Hsp70 expression increased significantly. A higher rate of apoptosis was observed in I/R group than in C + I/R group, and a significant increase of MDA, NO2^- + NO3^- and MPO level in liver tissues and serum transaminase concentration was also observed in I/R group compared to C + I/R group. Curcumin also decreased the activity of inducible NO synthase (iNOS) in liver after reperfusion,but had no effect on the level of endothelial NO synthase (eNOS) after reperfusion in liver. The 7 d survival rate was significantly higher in C + I/R group than in I/R group. CONCLUSION: Curcumin has protective effects against hepatic I/R injury. Its mechanism might be related to the overexpression of Hsp70 and antioxidant enzymes. 展开更多
关键词 ISCHEMIA Reperfusion injury CURCUMIN LIVER Protection
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Expression profiles of two small heat shock proteins and antioxidant enzyme activity in Mytilus galloprovincialis exposed to cadmium at environmentally relevant concentrations 被引量:5
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作者 由丽萍 宁璇璇 +4 位作者 陈磊磊 张林宝 赵建民 刘小莉 吴惠丰 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2014年第2期334-343,共10页
Small heat shock proteins encompass a widespread but diverse class of proteins, which play key roles in protecting organisms from various stressors. In the present study, the full-length cDNAs of two small heat shock ... Small heat shock proteins encompass a widespread but diverse class of proteins, which play key roles in protecting organisms from various stressors. In the present study, the full-length cDNAs of two small heat shock proteins (MgsHSP22 and MgsHSP24.1) were cloned from Mytilus galloprovincialis, which encoded peptides of 181 and 247 amino acids, respectively. Both MgsHSP22 and MgsHSP24.1 were detected in all tissues examined by real-time PCR, with the highest expression being observed in muscle and gonad tissues. The real-time PCR results revealed that Cd significantly inhibited MgsHSP22 expression at 24 h and MgsHSP24.1 at 24 and 48 h under 5 ug/L Cd2+ exposure. MgsHSP24.1 expression was also significantly inhibited after 50 ug/L Cd2+ exposure for 48 h. With regard to antioxidant enzymes, increased GPx and CAT activity were detected under Cd2+ stress (5 and 50 ug/L), while no significant difference in SOD activity was observed throughout the experiment. Overall, both MgsHsps and antioxidant enzymes revealed their potential as Cd stress biomarkers in M. galloprovincialis. 展开更多
关键词 Mytilus galloprovincialis small heat shock protein superoxide dismutase (SOD) catalase (CAT) glutathione peroxidase (GPx) CADMIUM
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Interaction of temperature and salinity on the expression of immunity factors in different tissues of juvenile turbot Scophthalmus maximus based on response surface methodology 被引量:5
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作者 黄智慧 马爱军 +5 位作者 王新安 雷霁霖 李伟业 王婷 杨志 曲江波 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2015年第1期28-36,共9页
Central Composite Design(CCD) and response surface methodology were used in the experiment to examine the combined effect of temperature(16-28℃) and salinity(18-42) on Hsp70 and IgM genes expression levels in turbot(... Central Composite Design(CCD) and response surface methodology were used in the experiment to examine the combined effect of temperature(16-28℃) and salinity(18-42) on Hsp70 and IgM genes expression levels in turbot(Scophthalmus maximus) liver and kidney.The results showed that the coefficients of determination(R^2=0.965 2 for liver Hsp70,0.972 9 for kidney Hsp70,0.921 for liver IgM and 0.962 1 for kidney IgM) and probability values(P<0.01) were significant for the regression model.The interactive effect between temperature and salinity on liver Hsp70,kidney Hsp70 and liver IgM were not significant(P>0.05),while the interactive effect between temperature and salinity on kidney IgM was significant(P<0.01).The model equation could be used in practice for forecasting Hsp70 and IgM genes expression levels in the liver and kidney of juvenile turbot via applying statistical optimization of the response of interest,at which the maximum liver Hsp70,kidney Hsp70,liver IgM and kidney IgM of1.48,1.49,2.48,and 1.38,respectively,were reached.The present model may be valuable in assessing the feasibility of turbot farming at different geographic locations and,furthermore,could be a useful reference for scientists studying the immunity of turbot. 展开更多
关键词 turbot (Scophthalmus maximus) response surface methodology (RSM) Central CompositeDesign (CCD) HSP70 immunoglobulin M (IgM)
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Measurement of Antibodies and Cytokines in Chlamydia Trachomatis-related Infertile Patients
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作者 王慧 熊礼宽 +1 位作者 袁凤娣 朱锦明 《Chinese Journal of Sexually Transmitted Infections》 2002年第4期29-32,共4页
Objectives: To find out the level and functions of Chlamydia trachomatis heat shock protein (C-hsp60) antibody, anti-spermantibody(ASAb), interleukin 1(IL-1), interleukin 6 (IL-6),interleukin 8 (IL-8), Tumor necrosis ... Objectives: To find out the level and functions of Chlamydia trachomatis heat shock protein (C-hsp60) antibody, anti-spermantibody(ASAb), interleukin 1(IL-1), interleukin 6 (IL-6),interleukin 8 (IL-8), Tumor necrosis factor alpha (TNF-α)and γ-interferon (IFN-γ) in patients with CT-related infertility. Methods: CT-DNA of cervical secretions was detectedthrough polymerase chain reaction (PCR) and migrationinhibiting factor (MIF) was employed to measure IgG titre ofCT MOMP antibody. Western blot was used to determinepresence of C-hsp60 antibody and enzyme-linkedimmunoadsorbent assay (ELISA) measured ASAb of IgG typein blood serum and determine the content of IL-1, IL-6. IL-8.TNF-α. IFN-γ in uterine tube fluid. Results: 68 patients had positive CT-DNA, among which 57(83.8%) had C-hsp60 antibody. Among the 172 patients withnegative CT-DNA, 64 patients (37.2%) also had C-hsp60Antibody. There was a significant difference (P<0.01) betweeninfertile patients and control group patients in the presence ofAAb. Infertile patients with positive CT-DNA had higher levels of IL-1、IL-6. IL-8. TNF-α. IFN-γ in uterine tube fluidcompared to control group patients (P<0.01). Conclusion: Firstly, those patients with negative CT testingfrom cervical secretions cannot be ruled out for CT infectionin deep parts of the body (such as oviduct, pelvic kidney).Detection of C-hsp60 Antibody may help to diagnose suchcases of CT. Secondly, CT infection of the oviduct can raiselevels of IL-1、IL-6、 IL-8、 TNF-α. IFN-γ. The pathogenesis ofinfertility caused by CT infection in the reproductive tractmay be related to cytokine production and inflammatoryresponses mediated by C-hsp60 Antibody, IL-1, IL-6, IL-8,TNF-α, and IFN-γ. 展开更多
关键词 Chlamydia Trachomatis (CT) ANTIBODY INFERTILITY CYTOKINE
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Mechanisms of resistance to trastuzumab:an updated review 被引量:1
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作者 Yan Zhang Jinsheng Zhang 《The Chinese-German Journal of Clinical Oncology》 CAS 2010年第11期660-665,共6页
Human epidermal growth factor receptor 2 (HER2) is over-expressed in 20%-25% of invasive breast cancer and is associated with an aggressive tumor phenotype and reduced survival rate. As a clinically widely applied H... Human epidermal growth factor receptor 2 (HER2) is over-expressed in 20%-25% of invasive breast cancer and is associated with an aggressive tumor phenotype and reduced survival rate. As a clinically widely applied HER2-targeted monoclonal antibody, trastuzumab (Herceptin), combined with chemotherapy significantly increases the no tumor survival time of the patient. However, the majority of the cancers that initially respond to Herceptin begin to counter against the treat- ment within 1 year. This review describes several important well-known trastuzumab resistance mechanisms as well as the updated advancement in this field. The sufficiently investigated mechanisms are over-activation of PI3K/AKT pathway, activa- tion of PI3K/AKT via alternative pathway, steric hindrance of receptor-antibody interaction, increase of serum HER2 extracel- lular domain, and abnormal expression of epidermal growth factor receptor (EGFR) family and their ligands. And the newly investigated mechanisms involve Darpp-32 and t-Darpp, autophagy of tumor cells, HSP27, HsP90, c-MYC, ADAM proteases, EphA2, Racl, MUCI*, HER2△16, and mesenchymal CD44(pos)CD24(neg/low) phenotype. 展开更多
关键词 human epidermal growth factor receptor 2 (HER2) TRASTUZUMAB resistance PI3K/AKT IGF-1R MUC4 epidermal growth factor receptor (EGFR) family
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