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抗犬瘟热病毒单克隆抗体的制备及初步应用 被引量:12
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作者 陈万荣 付少才 刘树玲 《中国兽医学报》 CAS CSCD 北大核心 2002年第5期455-456,共2页
采用 B淋巴细胞杂交瘤技术 ,通过免疫荧光法筛选 ,获得 4株能稳定分泌抗犬瘟热病毒的单克隆抗体杂交瘤细胞株。 4株单克隆抗体均属 Ig G1亚类 ,其腹水效价在 1∶ 10 - 4 ~ 1∶ 10 - 5,细胞培养上清液效价为 1∶ 10 - 2 ~ 1∶ 10 - 3 ... 采用 B淋巴细胞杂交瘤技术 ,通过免疫荧光法筛选 ,获得 4株能稳定分泌抗犬瘟热病毒的单克隆抗体杂交瘤细胞株。 4株单克隆抗体均属 Ig G1亚类 ,其腹水效价在 1∶ 10 - 4 ~ 1∶ 10 - 5,细胞培养上清液效价为 1∶ 10 - 2 ~ 1∶ 10 - 3 。用微量细胞培养中和试验筛选到 2株具有中和活性的特异性单克隆抗体 ,其中和效价为 1∶ 32和 1∶ 12 8,并初步应用于治疗中早期犬瘟热病毒感染犬 ,取得了较好的结果。 展开更多
关键词 抗犬瘟热 病毒 单克隆 应用 免疫荧光试验 治疗
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抗犬瘟热疫苗的研制及其效果
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作者 李琼璋 《畜牧兽医科技信息》 2000年第6期13-13,共1页
抗犬瘟热冻干苗是由-88株研制而成的,试验是在4个养兽场进行的,总共接种了165130只年龄在2—3和8—9个月的皮毛兽(杂种鼬、水貂、北极狐、狸)。
关键词 犬瘟热疫苗 北极狐 抗犬瘟热 体效价 检测 接种后 免疫原性 基础群 攻毒试验 存活率
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ELISA Detection of Antibody against Canine Distemper Virus in Fox
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作者 张艳英 卢会鹏 +5 位作者 王晓姗 郭杨柳 吴楠 李艳云 史秋梅 高超 《Agricultural Science & Technology》 CAS 2015年第1期120-124,共5页
Canine distemper is one of the most important common viral epidemics in farmed foxes. In this study, the whole blood samples of foxes that had been immunized with canine distemper vaccine were collected from a farming... Canine distemper is one of the most important common viral epidemics in farmed foxes. In this study, the whole blood samples of foxes that had been immunized with canine distemper vaccine were collected from a farming area in Changli County. The antibody against canine distemper virus in the 85 prepared serum samples were detected with a double-antibody sandwich ELtSA method. The results showed among the 85 samples from the 4 farms, 81 samples were antibody-positive, and only 4 samples had no antibody against canine distemper. The overall antibody level was higher in that farming area. It is indicated canine distemper vaccination and vaccination program have gotten attention from most of the local farmers, and the immune effect of canine distemper vaccine is better. 展开更多
关键词 Canine distemper in fox ANTIBODY ELISA
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