等离子体类聚氧乙烯薄膜的制备及性能研究

等离子体类聚氧乙烯薄膜(PEO-like)具有高亲水性、抗生物粘附的特性,能被应用于生物材料表面改性以提高材料的抗蛋白粘附、抗血小板粘附和抗菌等性能。以四乙二醇二甲醚(tetraethylene glycol dimethyl ether)为单体,采用射频等离子体连续放电模式,不锈钢SS为基底,在不同功率下合成了PEO-like薄膜。傅里叶变换红外光谱仪(FT-IR)、X光电子能谱仪(XPS)测试分析表明等离子体PEO-like薄膜中能部分保留单体分子中的氧化乙烯(EO)结构单元,功率为5W合成的PEO-like薄膜中EO单元含量为35.86%。PEO-like薄膜的亲水性优于不锈钢,薄膜在双蒸水中浸没1和10d的结果显示,尽管薄膜出现溶胀现象,但没有剥落和破裂。低功率下合成的薄膜具有一定的阻抗血小板粘附的性能,但3d的体外平滑肌细胞评价结果表明,3种PEO-like薄膜没有表现出抑制平滑肌细胞增殖的性能。

基于两性离子聚合物的防污涂层的探究

通过层层自组装法将聚磺酸甜菜碱、单宁酸和Fe~Ⅲ互相结合,在基材表面构建两性离子涂层并对其进行亲水性和抗污损性能表征。实验结果表明:随着组装层数的增加,水接触角从50°下降至15°;当组装层数为5时,在基材表面形成了厚度为55 nm的涂层;将其浸泡于蛋白质溶液后,在荧光显微镜下该涂层表面没有产生明亮的绿色荧光,说明该涂层具备良好的抗污损性能。

Synthesis of two multivalent lactosides with anti-adhesive activity and their fluorescein-labeled and biotin-labeled derivatives

Adhesion of leukocytes to endothelium plays an important role in inflammation-associated diseases.Our previous studies showed that multivalent lactosides were able to inhibit this process.Using 2-azide-l,3-propandiol and glutamic acid as spacers,we synthesized divalent lactoside An-2 and tetravalent lactoside Gu-4 by means of convergent method.These two compounds displayed high anti-adhesive activity and showed therapeutic effect in rats with severe burn shock.In addition, investigation of the anti-adhesion biological mechanism using labeled compounds YAn-2 and YGu-4 demonstrated that the target of multivalent lactosides was CD11b,theβ2 integrin subunit,on the surface of leukocytes.In this paper,the synthesis of these two new multivalent lactosides as well as their fluorescein-labeled and biotin-labeled compounds is reported.