In order to determine the optimal mode of capillary electrophoresis for the impurity control of β-lactam antibiotics, different modes and various electrophoresis conditions for the separation of impurities were compa...In order to determine the optimal mode of capillary electrophoresis for the impurity control of β-lactam antibiotics, different modes and various electrophoresis conditions for the separation of impurities were compared.The results showed that micellar electrokinetic capillary chromatography（MEKC） was the optimal separation mode for the impurity profiling of β-lactam antibiotics.In MEKC,not only the common R and S isomers,Δ-2 andΔ-3 isomers,and Z and E isomers,but also the impurities of β-lactam antibiotics could be well separated compared with the capillary zone electrophoresis.Therefore,MECK is the first choice for the separation of impurities of β-lactam antibiotics with capillary electrophoresis（CE）.The optimal separation could be achieved in MEKC by optimizing the pH and the concentrations of buffered saline,micelles and organic solvent（methanol） in running buffer.展开更多
基金National Key New Drug R&D Program Foundation of China(Grant No.2009ZX09313-027)
文摘In order to determine the optimal mode of capillary electrophoresis for the impurity control of β-lactam antibiotics, different modes and various electrophoresis conditions for the separation of impurities were compared.The results showed that micellar electrokinetic capillary chromatography（MEKC） was the optimal separation mode for the impurity profiling of β-lactam antibiotics.In MEKC,not only the common R and S isomers,Δ-2 andΔ-3 isomers,and Z and E isomers,but also the impurities of β-lactam antibiotics could be well separated compared with the capillary zone electrophoresis.Therefore,MECK is the first choice for the separation of impurities of β-lactam antibiotics with capillary electrophoresis（CE）.The optimal separation could be achieved in MEKC by optimizing the pH and the concentrations of buffered saline,micelles and organic solvent（methanol） in running buffer.
