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食源性致病菌药敏性检测用参考菌株的研制
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作者 秦明倩 杨静 +4 位作者 赵红阳 卢行安 周巍 崔生辉 杨保伟 《中国食品卫生杂志》 CSCD 北大核心 2021年第6期666-672,共7页
目的鉴于当前食源性致病菌药敏性测定用质控菌对常用抗生素较敏感,而我国分离的大部分菌株对常用抗生素较耐受,药敏性测定时需选择的抗生素浓度范围非常大,导致抗生素浪费,测试结果不准确。方法本研究研制了5种抗生素最小抑菌浓度(MIC)... 目的鉴于当前食源性致病菌药敏性测定用质控菌对常用抗生素较敏感,而我国分离的大部分菌株对常用抗生素较耐受,药敏性测定时需选择的抗生素浓度范围非常大,导致抗生素浪费,测试结果不准确。方法本研究研制了5种抗生素最小抑菌浓度(MIC)赋值明确、可用于食源性致病菌(乌普萨拉沙门氏菌、苏卜拉沙门氏菌、汤普森沙门氏菌、印第安纳沙门氏菌、肠炎沙门氏菌)对常见抗生素(链霉素、庆大霉素、氯霉素、头孢西丁、氨苄西林等)药敏性检测用的菌株标准样品。采用琼脂稀释法和微量肉汤稀释法测定菌株的药敏性,使用真空冷冻干燥方法制备参考菌株标准样品。结果分别研制得到链霉素、庆大霉素、氯霉素、头孢西丁、氨苄西林MICs赋值明确的参考菌株,菌株药敏性遗传稳定、均匀性和贮存稳定性良好。分别在37℃、4℃和-80℃条件下贮存13、90、360 d后,活菌量基本维持在10^(5)CFU/瓶以上,确定的耐药表型均可检出,抗生素MICs值未出现较大波动或漂移。结论符合我国食源性致病菌耐药特性、抗生素MICs赋值明确菌株标准样品的研制,将有助于丰富我国参考菌株种类,可以进一步加强耐药菌监测,确保食品安全。 展开更多
关键词 参考菌株 抗生素药敏性 均匀 储存稳定
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Comparative Study of the Static Magnetic Field Effects on Growth Rate with Relative Antibiotic Susceptibility in Escherichia coli
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作者 Fouad Houssein Kamel Ashti M. Amin Khonaw Kader Salih Saleem S.Qader 《Journal of Life Sciences》 2013年第7期690-694,共5页
We studied the biological effects of different magnetic fields. Identified bacterial strain Escherichia coli (type I) has been exposed to the dipolar magnetic field force (400, 800, 1200 and 1600 Gausses) which pr... We studied the biological effects of different magnetic fields. Identified bacterial strain Escherichia coli (type I) has been exposed to the dipolar magnetic field force (400, 800, 1200 and 1600 Gausses) which prepared locally with incubation for different period times (24, 48 and 72 hrs) at 37℃. The effects were evaluated by optical density (OD) at 600 nm determining their growth density incorporation with negative control and depending of McFarland turbidity standard (0.5), in addition to its susceptibility to various antibiotics. Results illustrate different forces of magnetic field decreased the growth rate of E. coli in particular at 24 hrs incubation comparing with unexposed or control samples. The magnetic field increased the logarithmic phase within 4-6 hrs of treatment but decreased after 16 to 18 hrs. Furthermore, changes in the antibiotic sensitivity were observed after exposure period of 6 hrs since E. coli cells became more sensitive to certain antibiotics. While after a 16 hrs exposure period, it became more resistant to the same antibiotics comparing with control groups. 展开更多
关键词 Magnetic field BACTERIA optical density Escherichia coli antibiotic susceptibility.
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Occurrence and Antibiotic Susceptibility Profile of Streptococcus spp. Isolated from Ewe's Milk
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作者 Athanasios Alexopoulos Stavros Plessas +3 位作者 Zafiris Abas Vasiliki Lagka Antonis Zdragas Eugenia Bezirtzoglou 《Journal of Food Science and Engineering》 2013年第12期657-661,共5页
The purpose of this study was to determine the occurrence and antimicrobial resistance of Streptococcus spp. isolated from fresh ewe's milk and consequently to draw conclusions concerning the health condition of the ... The purpose of this study was to determine the occurrence and antimicrobial resistance of Streptococcus spp. isolated from fresh ewe's milk and consequently to draw conclusions concerning the health condition of the animal flocks and the current trends of antibiotic use in small ruminants. A total of 77 mountainous sheepfolds were investigated representing 10% of all transhumant sheep and goats flocks in the area. Duplicate milk samples were collected from January to February transferred to the laboratory and analyzed for the presence of Streptococcus spp. by using the serial dilution method and plating on the surface of Slanetz and Bartley agar. The isolates were tested against nine antimicrobial agents with the Kirby-Bauer disk diffusion method. The results showed that Streptococcus spp. occurred in 84.4% of the samples and in counts ranged between 1.8 log CFU/mL and 4.6 log CFU/mL. Concerning susceptibility, only 1.3% of the isolates were resistant to all antimicrobials while 27.3% were fully susceptible. Resistance to cefepime was the most common (93.1%) observation followed by resistance to ampicillin (82.1%), ciprofloxacine (58.3%) and meropenem (51.9%). The isolates were vancomycin susceptible at a rate of 83.6%. Multidrug resistance to at least three antibiotics was observed to be 22.1% of Streptococcus spp.. Given the prevalence of Streptococcus spp. and the elevated resistance of isolates to various antibiotics it is concluded that further examinations and even interventions are required in order to pertain the appropriate animal health and milk quality. 展开更多
关键词 Antibiotic ewe's milk Streptococcus.
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