云南省地方油菜品种资源鉴定及研究

对云南省 317份油菜种质资源进行研究 ,发现云南省油菜种质资源大部分为极早熟和早熟品种 ,占云南油菜品种资源的 75 % ;同时也是高产种质资源和高含油量种质资源 ,且含油量与海拔高度有关 ,即高海拔地区油菜种质的含油量普遍较高 ;该省地方品种中缺乏低芥酸(<1% )和低硫代葡萄糖甙 (<30 μmol/g)的种质资源 ,研究结果为育种工作的准确性和可靠性提供科学依据。

我国葡萄属野生种对炭疽病抗性的研究

利用田间自然鉴定.田间接种鉴定和室内果实离体鉴定三种方法.研究了起源于我国的葡萄属12种或变种、49个株系对葡萄炭疽病的抗性.结果表明.一些种和株系的果实不感炭疽病或感病率极低.表现为抗(?)性极强或强.严重感病的仅是个别株系.我国葡萄属植物是极为宝贵的抗炭疽病的种质资源.

福建省褐稻虱生物型研究

采用具有不同抗性基因的 5个水稻鉴别品种 ,把从宁化、诏安、建阳、平和、莆田县黄石和笏石、德化县上涌和城关、周宁、武平等县田间采集的褐稻虱分别接在 5个鉴别稻种上进行福建省不同地区褐稻虱生物型的测定 ,结果表明 ,TN1受害比其他 4个稻种严重 ;不同地区褐稻虱在 TN1上的生存率、群体数量、蜜露排泄量均多于其他 4个品种 ,表明 8个县 10个点褐稻虱属生物型 1。文章根据生物型测定结果 。

Identification of Drought Tolerance in Peanut (Arachis hypogaea) Germplasm at Seedling Stage

[Objective] This study was conducted to identify the drought tolerance of peanut（Arachis hypogaea） seedlings. [Method] The plant height, fresh weight and dry weight of 80 peanut varieties or lines were determined under the drought stress simulated using 15% PEG6000 in laboratory. Relative values of the three indices were calculated to measure the drought tolerance of the 80 peanut varieties or lines. [Result] The growth of peanut seedlings was significantly inhibited after 10 d of drought treatment. Cluster analysis revealed that at Euclidean distance D=15, the80 peanut varieties or lines were divided into four categories A, B, C and D. Among them, category B had better drought tolerance while category C was sensitive to drought. In all the four categories, the approved peanut varieties or lines showed better drought tolerance than the unapproved ones. [Conclusion] The drought tolerant and the drought sensitive peanut varieties or lines screened in this study can be used for further basic research and germplasm improvement.

Research Progress and Identification Method of Apple Stress Resistance

In the research, changes of apple chemistry, and molecule, under stresses, are n terms of morphology, physiology, bio- illustrated and research and identifica- tion methods of apple resistance are explored involving drought-resistance, flood-re- sistance, salt-stress resistance, cold-hardiness and heat-resistance. In addition prospects of apple resistance research are proposed, as well.

Isolation,characterization and extraction of mer gene of Hg^(2+) resisting strain D_2

Mercury-resistant strain D2 was isolated from mercury-contaminated soil and investigations on its 16S rDNA sequence,growth,minimal inhibitory concentrations(MICs) of metals,antibiotic susceptibility and mer gene were conducted.The strain D2 can grow in the medium containing 60 mg/L mercury ion.It presents more than 99% identity of 16S rRNA gene with Pseudomonas aeruginosa,and exhibits high MIC values for heavy metals and a large spectrum antibiotics resistance.The mer RT gene sequence was amplified from chromosome.Strain D2 is identified as Pseudomonas aeruginosa and the resistance to mercuric ion is related to chromosome.

Screening and characterization of a high taxol producing fungus by protoplast mutagenesis

The preparation, regeneration and mutagenesis of the taxol-producing fungus UV40-19 protoplasts were discussed in the experiment. Totally 42 strains displayed hygromycin resistance. Six strains were found to be positive mutants when screened on plate containing 90μg/mL hygromycin. One hereditarily stable strain UN05-6 was obtained, which raised the taxol yield from （376.38 ± 8.41）μg/L to （493.12 ±11.36） μg/L. The optimal conditions for the preparation, regeneration and mutagenesis of the taxol producing fungus UV40-19 were as follows： 1 ）enzymolysis in a solution containing 3% lywallzyme, 4% snailase, 1% lysozyme and 3% cellulose at 30~C water bath, pH5.5 - 6.0 for 5h; 2） The prepared protoplasts were regenerated by using bilayer plate culturing method; 3）To mutagenize the fungus UV40-19, the protoplast suspension was treated with 0.8mg/mL NTG for 15min, followed by UV irradiation （30W, 30cm distance）for 40s under magnetic stirring. The purified products of the fungus UN05-6 fermented extracts have significant inhibitive effects on SMMC-7721 cell.

Antibiotic resistance mechanisms of Myroides sp.

Bacteria of the genus Myroides （Myroides spp.） are rare opportunistic pathogens. Myroides sp. infections have been reported mainly in China. Myroides sp. is highly resistant to most available antibiotics, but the resistance mechanisms are not fully elucidated. Current strain identification methods based on biochemical traits are unable to identify strains accurately at the species level. While 16S ribosomal RNA （rRNA） gene sequencing can accurately achieve this, it fails to give information on the status and mechanisms of antibiotic resistance, because the 16S rRNA sequence contains no information on resistance genes, resistance islands or enzymes. We hypothesized that ob- taining the whole genome sequence of Myroides sp., using next generation sequencing methods, would help to clarify the mechanisms of pathogenesis and antibiotic resistance, and guide antibiotic selection to treat Myroides sp. infec- tions. As Myroides sp. can survive in hospitals and the environment, there is a risk of nosocomial infections and pandemics. For better management of Myroides sp. infections, it is imperative to apply next generation sequencing technologies to clarify the antibiotic resistance mechanisms in these bacteria.