HIV/AIDS is one of the most serious public health challenges globally. Despite the great efforts that are being devoted to prevent,treat and to better understand the disease,it is one of the main causes of morbidity a...HIV/AIDS is one of the most serious public health challenges globally. Despite the great efforts that are being devoted to prevent,treat and to better understand the disease,it is one of the main causes of morbidity and mortality worldwide. Currently,there are 30 drugs or combinations of drugs approved by FDA. Because of the side-effects,price and drug resistance,it is essential to discover new targets,to develop new technology and to find new anti-HIV drugs. This review summarizes the major targets and assays currently used in anti-HIV drug screening.展开更多
文摘目的：建立UPLC-MS/MS方法检查中药制剂中非法添加抗病毒化学成分阿昔洛韦、金刚烷胺及利巴韦林。方法：色谱柱为ACQUITY UPLC BEH-C_（18）（2.1mm×50mm,1.7μm）,梯度洗脱：流动相A为10mmol/L的乙酸铵溶液,流动相B为甲醇,流速为0.3 m L/min。正离子扫描,扫描方式采用全扫描一级质谱、全扫描二级质谱,质荷比范围50~500。结果：发现一种中药掺有阿昔洛韦。结论：本法简便、快速、准确,可用灵敏的检出中药中添加阿昔洛韦、金刚烷胺及利巴韦林等化学成分。
文摘HIV/AIDS is one of the most serious public health challenges globally. Despite the great efforts that are being devoted to prevent,treat and to better understand the disease,it is one of the main causes of morbidity and mortality worldwide. Currently,there are 30 drugs or combinations of drugs approved by FDA. Because of the side-effects,price and drug resistance,it is essential to discover new targets,to develop new technology and to find new anti-HIV drugs. This review summarizes the major targets and assays currently used in anti-HIV drug screening.
