耐热多抗紫长茄淄茄一号的丰产及抗病试验

鲁中地区春淡季茄子供应来源于南部山旱地区。近年来,茄子越夏栽培成熟期长,以黄萎病和绵疫病等为主的病害严重影响和制约了茄子产量。因此,选用优质抗病茄子品种成为解决地区茄子产量及品质的关键措施。淄博市农业科学研究院2009年育成的审定品种淄茄一号通过山东省区域及生产试验、抗病试验,表现杂交优势明显,对黄萎病、绵疫病及褐纹病等病害表现多抗,综合性状优良。

转基因抗病毒烟草的田间抗病性试验

烟草花叶病是烟草的重要病害之一，利用黄瓜花叶病毒（ＣＭＶ）和烟草花叶病毒（ＴＭＶ）的外壳蛋白（ＣＰ）基因培育抗病转基因烟草已获得成功，并已在生产上应用，而利用病毒编码的运动蛋白（ＭＰ）基因，构建转基因植物，则是一种新的抗病毒策略。我们与中国农业大学等...

水稻生产应用激活蛋白抗病效果试验初报

稻瘟病、稻曲病和立枯病是影响本地水稻生产的三大主要病害，对稻米的产量和品质造成不同程度影响。为探索传统农药的更新换代产品．试验激活蛋白对这三类病害的防治效果．我们通过和中国农科院植保所及同昕绿源（北京）生物科技股份有限公司合作，于2010年在本区进行多点激活蛋白防治痛害效果试验，从防病效看，该生物制剂对提高水稻免疫力具有很好的防治和治疗效果。

设施草莓生产底施五种有益微生物菌种的试验研究

为了掌握有益微生物菌种对设施草莓生产的增产效果,试验研究了底施有益微生物菌种对设施草莓产量、生长情况及抗病性的影响。试验结果表明,以复合EM菌种(有效活菌数≥1000亿/g)1 kg/667 m^(2)、枯草芽孢杆菌+地衣芽孢杆菌(有效活菌数≥200亿/g)复合菌种1 kg/667 m^(2)、多粘类芽孢杆菌(有效活菌数≥100亿/g)2 kg/667 m^(2)、枯草芽孢杆菌(有效活菌数≥1000亿/g)1 kg/667 m^(2),底施均可增强草莓的抗病性,具有一定的增产效果。其中以底施复合EM菌种(有效活菌数≥1000亿/g)1 kg/667 m^(2),产量最高,增产12.28%;枯草芽孢杆菌+地衣芽孢杆菌(有效活菌数≥200亿/g)复合菌种1 kg/667 m^(2)、多粘类芽孢杆菌(有效活菌数≥100亿/g)2 kg/667 m^(2)、解淀粉芽孢杆菌(有效活菌数≥1000亿/g)1 kg/667 m^(2)、枯草芽孢杆菌(有效活菌数≥1000亿/g)1 kg/667 m^(2),分别增产11.84%、10.38%、8.96%、8.52%。底施不同有益微生物菌种均使草莓增强抗病性,均未发生根腐病和端头病等病害,未发现死苗烂棵现象,而A6(CK)根腐病、断头病发病率分别为12%、5%,死苗率为15%,5种有益微生物菌种均可在设施草莓生产中推广使用。

百合种质资源对枯萎病的抗性鉴定

采用鳞片接种法对收集、保存的40个百合种质资源进行镰刀菌枯萎病的抗性鉴定.结果表明:高抗品种(种)有12个,中抗品种(种)17个;中感品种(种)7个,高感品种(种)4个.鳞片接种法可作为百合枯萎病抗性鉴定的基本方法.

Serological survey on canine coronavirus antibodies in giant pandas by virus neutralization test

In order to survey the infectious situation of canine coronavirus (CCV) in giant panda population, a virus neutralization test detecting specific antibodies against CCV in giant panda抯 sera was established by using two-fold dilutions of serum and 100 TCID50 of the virus. The 62 sera samples of giant pandas, which were gathered from zoos and reserve region of Sichuan Province, China were detected. The neutralization antibody titer of 1:4 was recognized as the positive criterion, 8 sera samples were detected to be positive, and the positive rate was 12.9%. The titers of neutralizing antibody ranged from 1:8 to 1:32. It was the first comprehensive investigation on neutralization antibodies against CCV in giant panda population in China. The results of study showed that the infection of CCV in giant panda population was universal, which has posed a threat to the health of giant panda. Therefore, it is incumbent on us to study safe and effective vaccines to protect giant panda against CCV infection.

Inhibition of hepatitis B virus replication by pokeweed antiviral protein in vitro

AIM: To explore the inhibitory effects of pokeweed antiviral protein seed (PAP-S) and PAP encoded by a eukaryotic expression plasmid on hepatitis B virus (HBV) replication in vitro. METHODS: HepG2 2.2.15 cells in cultured medium were treated with different concentrations of PAP-S. HBsAg, HBeAg and HBV DNA in supernatants were determined by ELISA and fluorescent quantitative PCR respectively. MTT method was used to assay for cytotoxicity. HepG2 were cotransfected with various amounts of PAP encoded by a eukaryotic expression plasmid and replication competent wild-type HBV 1.3 fold over- length plasmid. On d 3 after transfection, HBsAg and HBeAg were determined by using ELISA. Levels of HBV core-associated DNA and RNA were detected by using Southern and Northern blot, respectively. RESULTS: The inhibitory effects of PAP-S on HBsAg, HBeAg and HBV DNA were gradually enhanced with the increase of PAP concentration. When the concentration of PAP-S was 10 μg/mL, the inhibition rates of HBsAg, HBeAg and HBV DNA were 20.9%, 30.2% and 50%, respectively. After transfection of 1.0 μg and 2.0 μg plasmid pXF3H-PAP, the levels of HBV nucleocapside- associated DNA were reduced by 38.0% and 74.0% respectively, the levels of HBsAg in the media by 76.8% and 99.7% respectively, and the levels of HBeAg by 72.7% and 99.3% respectively as compared with controls. Transfection with 2 μg plasmid pXF3H-PAP reduced the levels of HBV nucleocapside-associated RNA by 69.0%.CONCLUSION: Both PAP-S and PAP encoded by a eukaryotic expression plasmid could effectively inhibit HBV replication and antigen expression in vitro, and the inhibitory effects were dose-dependent.

Could quantitative liver function tests gain wide acceptance among hepatologists?

It has been emphasized that the assessment of residual liver function is of paramount importance to determine the following： severity of acute or chronic liver diseases independent of etiology; long-term prognosis; step-bystep disease progression; surgical risk; and efficacy of antiviral treatment. The most frequently used tools are the galactose elimination capacity to asses hepatocyte cytosol activity, plasma clearance of indocyanine green to assess excretory function, and antipyrine clearance to estimate microsomal activity. However, a widely accepted liver test （not necessarily a laboratory one） to assess quantitative functional hepatic reserve still needs to be established, although there have been various proposals. Furthermore, who are the operators that should order these tests？ Advances in analytic methods are expected to allow quantitative liver function tests to be used in clinical practice.

Comparative Experiments of Potato Resistance to Potato Late Bright in Zhaotong City

A comparative test was conducted on 6 potato varieties in Songshan Vil- lage in Zhaoyang District during 3 years, and PB06, a new variety, was selected, which provides references for selecting highly-yielding, highly-qualified and late blight- tolerant potatoes.

港口工程设计中桩基冰荷载计算探讨

在透空式的高桩码头以及墩式码头在运行的过程中,冰荷载会对整个结构产生非常大的负面影响,在很多港口工程设计的过程中,冰荷载通常是控制整个工程荷载以及冰荷载对码头桩基影响分析的一个非常重要的部分,因此我们需要对其予以高度的重视。主要分析了港口工程设计中桩基冰荷载计算,以供参考和借鉴。

Development of Novel Therapeutics for Chronic Hepatitis B

Chronic infection of hepatitis B virus (HBV) presents one of the serious public health challenges worldwide. Current treatment of chronic hepatitis B (CHB) is limited, and is composed of interferon and nucleoside/nucleotide reverse transcriptase inhibitors (NRTI). Interferon is poorly tolerated and is only responsive in a small fraction of CHB patients and NRTIs often face the problem of emergence of drug resistance during long-term treatment. The current treatment of CHB can be improved in several ways including genotyping mutations associated with drug resistance before treatment to guide the choice of NRTIs and suitable combinations among NRTIs and interferon. It is important to continue research in the identification of novel therapeutic targets in the life cycle of HBV or in the host immune system to stimulate the development of new antiviral agents and immunotherapies. Several antiviral agents targeting HBV entry, cccDNA, capsid formation, viral morphogenesis and virion secretion, as well as two therapeutic vaccines are currently being evaluated in preclinical studies or in clinical trials to assess their anti-HBV efficacy.

Performance of Chinese vegetable—Sweet Rape Flower 31 in Ethiopia

This paper describes the yield performance, plant characters, and insect pest and disease resistances of Chinese vegetable—Sweet Rape Flower 31 under Ethiopia conditions.