The resistance of a newly bred line 4163 containing the rice blast resistance gene P/40 and another 11 monogenic lines to rice blast in natural disease nurseries was identified. The results showed that 4163 was resist...The resistance of a newly bred line 4163 containing the rice blast resistance gene P/40 and another 11 monogenic lines to rice blast in natural disease nurseries was identified. The results showed that 4163 was resistant to rice blast isolates from Yunnan, and the leaf blast resistance levels were at grades 0, 0 and 4 in Baoshan City, Yiliang City and Yuxi City respectively, while the ear blast resistance was at level 3. Meanwhile, the resistance spectrum of Pi40 to 49 representative rice blast isolates from Yunnan Province was studied by indoor inoculation and was compared with another 25 resistance genes. The results revealed that the resistance frequency of Pi40 to the 49 typical isolates reached 87.8%, showing a broader resistance spectrum, so it could be used as a new source of resistance genes in breeding of japonica rice in Yunnan plateau in the future.展开更多
[Objective] It is to clone broad-spectrum anti-disease gene NPR1 and to construct its protein expression vector.[Method] First to extract total RNA of Arabidopsis thaliana and design relevant primers,and then the meth...[Objective] It is to clone broad-spectrum anti-disease gene NPR1 and to construct its protein expression vector.[Method] First to extract total RNA of Arabidopsis thaliana and design relevant primers,and then the method of reverse transcription PCR was adopted to clone.With the method of enzyme digestion and ligation,this gene will be directed into protein expression vector.[Result] After relevant testing,NPR1 was inserted into vector pMXB10 to obtain pMXB10-NPR1 protein expression vector.[Conclusion] Protein expression vector including NPR1 was successfully constructed.展开更多
NPR1 is an intersection of different disease resistance pathways and plays a key role in systemic-acquired resistance and induced systemic resistance. NPR1 regulates constitutive resistance and disease resistance dete...NPR1 is an intersection of different disease resistance pathways and plays a key role in systemic-acquired resistance and induced systemic resistance. NPR1 regulates constitutive resistance and disease resistance determined by resistant genes. In this paper, the mechanism of disease resistance of NPR1 genes and its analogs were described. The research status and progress of NPR1 transgenic crops were systematically discussed. The application prospect of NPR1 transgenic crops was also forecasted.展开更多
Staphylococcus aureus is major human pathogen causing large variety of infections worldwide. This study carried out to isolate S. aureus from different clinical cases, also detection of MRSA prevalence and VRSA emerge...Staphylococcus aureus is major human pathogen causing large variety of infections worldwide. This study carried out to isolate S. aureus from different clinical cases, also detection of MRSA prevalence and VRSA emergence, in addition to shedding light on strains that have to be multidrug resistance against various antibiotics, The clinical samples were collected from AI-Jumhuory Teaching Hospital patients in Mosul, isolates identification were achieved by conventional procedures including biochemical and physiological tests, and the specific latex agglutination test. The sensitivity pattern achieved by using disk diffusion technique, for MRSA and VRSA detection oxacillin-disk （1 μg） and vancomycin-disk （30 μg） were used respectively. Results revealed, among 17 S. aureus isolates, 7 （41%） were mostly isolated from patients with wound and burn infections. Isolates had high resistance rate against ampicillin （100%） and cefotaxime （81%）, and lower resistance rate against several antibiotics. MRSA was 88% of total isolates, 93.3% of MRSA were multidrug resistance to 3-9 of antibiotics. Six isolates （40%） of MRSA were VRSA. It is concluded that antibiotics other than vancomycin can be used as anti-MRSA agents after a sensitivity test to prevent the prevalence of VRSA, the major cause of this chemotherapy problems maybe irrational and indiscriminate use of broad-spectrum antibiotics.展开更多
Recently microwave-induced chemical synthesis of curcumin-metformin adduct to enhance the efficacy of metformin in preventing the formation of Advanced Glycation End Products （AGEs） has been reported from authors＇ ...Recently microwave-induced chemical synthesis of curcumin-metformin adduct to enhance the efficacy of metformin in preventing the formation of Advanced Glycation End Products （AGEs） has been reported from authors＇ laboratory. The present studies describe microwave-induced chemical synthesis and mass spectral characterization of curcumin-phenformin adducts using LC-MS/MS. The mechanism of formation and its analytical data via Thin-Layer Chromatography （TLC） combined with MS/MS fragmentation revealed a major six membered ring adduct and a minor eight membered ring isomer. A facile chemical synthesis and identification of major and minor isomers presented in this study may offer novel therapeutic strategies for inhibiting AGEs as well as anti-cancer treatments.展开更多
Dengue virus(DENV)is the most common mosquito-borne flavivirus,and it affects millions of people globally every year.Currently,there are no approved drugs for the treatment of dengue infection.By screening a natural p...Dengue virus(DENV)is the most common mosquito-borne flavivirus,and it affects millions of people globally every year.Currently,there are no approved drugs for the treatment of dengue infection.By screening a natural product library,we identified a novel compound,cyclovirobuxine D(Cvb D),that displays anti-DENV activity.Cvb D inhibits DENV replication in vitro in a dose-dependent manner and protects suckling mice against lethal DENV infection.Mechanistically,Cvb D regulates the expression of genes related to the cellular cholesterol pathway.As a result,Cvb D increases cellular cholesterol synthesis and accumulation,activates mTOR,and inhibits viral-dependent autophagy.Cvb D does not suppress autophagy initiation but impedes the nuclear translocation of the lysosome transcription factor TFEB.In addition,Cvb D restricts the replication of other positive-strand RNA viruses such as Zika virus and Coxsackievirus B3.We speculate that Cvb D could be a broad-spectrum antiviral drug candidate for use against positive-strand RNA viruses that require autophagy for optimal replication.展开更多
基金Supported by Project of Basic Research for Application in Yunnan Province,China(2010ZC174)Science and Technology Planning Project of Yunnan Province,China(2015ZA003)~~
文摘The resistance of a newly bred line 4163 containing the rice blast resistance gene P/40 and another 11 monogenic lines to rice blast in natural disease nurseries was identified. The results showed that 4163 was resistant to rice blast isolates from Yunnan, and the leaf blast resistance levels were at grades 0, 0 and 4 in Baoshan City, Yiliang City and Yuxi City respectively, while the ear blast resistance was at level 3. Meanwhile, the resistance spectrum of Pi40 to 49 representative rice blast isolates from Yunnan Province was studied by indoor inoculation and was compared with another 25 resistance genes. The results revealed that the resistance frequency of Pi40 to the 49 typical isolates reached 87.8%, showing a broader resistance spectrum, so it could be used as a new source of resistance genes in breeding of japonica rice in Yunnan plateau in the future.
基金Supported by project of Beijing Municipal Education Commission(KM200910020014)Project of Sand Control Department,Beijing Municipal Landscape Greening Bureau(2008)~~
文摘[Objective] It is to clone broad-spectrum anti-disease gene NPR1 and to construct its protein expression vector.[Method] First to extract total RNA of Arabidopsis thaliana and design relevant primers,and then the method of reverse transcription PCR was adopted to clone.With the method of enzyme digestion and ligation,this gene will be directed into protein expression vector.[Result] After relevant testing,NPR1 was inserted into vector pMXB10 to obtain pMXB10-NPR1 protein expression vector.[Conclusion] Protein expression vector including NPR1 was successfully constructed.
基金Supported by Natural Science Foundation of Hebei Province(C2013301069)Financial Special Projects of Hebei Province(2012055004,2014055205,F16R2016024592)+2 种基金Key Technology Support Program of Hebei Province(16226303D-3)The Industry System of Hebei Province(HBCT2013030207)The State Key Laboratory of Cotton Biology(CB2016A19)~~
文摘NPR1 is an intersection of different disease resistance pathways and plays a key role in systemic-acquired resistance and induced systemic resistance. NPR1 regulates constitutive resistance and disease resistance determined by resistant genes. In this paper, the mechanism of disease resistance of NPR1 genes and its analogs were described. The research status and progress of NPR1 transgenic crops were systematically discussed. The application prospect of NPR1 transgenic crops was also forecasted.
文摘Staphylococcus aureus is major human pathogen causing large variety of infections worldwide. This study carried out to isolate S. aureus from different clinical cases, also detection of MRSA prevalence and VRSA emergence, in addition to shedding light on strains that have to be multidrug resistance against various antibiotics, The clinical samples were collected from AI-Jumhuory Teaching Hospital patients in Mosul, isolates identification were achieved by conventional procedures including biochemical and physiological tests, and the specific latex agglutination test. The sensitivity pattern achieved by using disk diffusion technique, for MRSA and VRSA detection oxacillin-disk （1 μg） and vancomycin-disk （30 μg） were used respectively. Results revealed, among 17 S. aureus isolates, 7 （41%） were mostly isolated from patients with wound and burn infections. Isolates had high resistance rate against ampicillin （100%） and cefotaxime （81%）, and lower resistance rate against several antibiotics. MRSA was 88% of total isolates, 93.3% of MRSA were multidrug resistance to 3-9 of antibiotics. Six isolates （40%） of MRSA were VRSA. It is concluded that antibiotics other than vancomycin can be used as anti-MRSA agents after a sensitivity test to prevent the prevalence of VRSA, the major cause of this chemotherapy problems maybe irrational and indiscriminate use of broad-spectrum antibiotics.
文摘Recently microwave-induced chemical synthesis of curcumin-metformin adduct to enhance the efficacy of metformin in preventing the formation of Advanced Glycation End Products （AGEs） has been reported from authors＇ laboratory. The present studies describe microwave-induced chemical synthesis and mass spectral characterization of curcumin-phenformin adducts using LC-MS/MS. The mechanism of formation and its analytical data via Thin-Layer Chromatography （TLC） combined with MS/MS fragmentation revealed a major six membered ring adduct and a minor eight membered ring isomer. A facile chemical synthesis and identification of major and minor isomers presented in this study may offer novel therapeutic strategies for inhibiting AGEs as well as anti-cancer treatments.
基金the Priority Academic Program Development of Jiangsu Higher Education InstitutionsProgram for Changjiang Scholars and Innovative Research Team in University(PCSIRT)+4 种基金the National Natural Science Foundation of China(31770933 and 81971917)the Natural Science Foundation of Colleges in Jiangsu Province(17KJA310005)the Open Project Fund from State Key Laboratory of Genetic Engineering,Fudan University(SKLGE1903)the Key Laboratory of Reproduction Regulation of NHC(KF2018-01)a grant from Soochow Securities。
文摘Dengue virus(DENV)is the most common mosquito-borne flavivirus,and it affects millions of people globally every year.Currently,there are no approved drugs for the treatment of dengue infection.By screening a natural product library,we identified a novel compound,cyclovirobuxine D(Cvb D),that displays anti-DENV activity.Cvb D inhibits DENV replication in vitro in a dose-dependent manner and protects suckling mice against lethal DENV infection.Mechanistically,Cvb D regulates the expression of genes related to the cellular cholesterol pathway.As a result,Cvb D increases cellular cholesterol synthesis and accumulation,activates mTOR,and inhibits viral-dependent autophagy.Cvb D does not suppress autophagy initiation but impedes the nuclear translocation of the lysosome transcription factor TFEB.In addition,Cvb D restricts the replication of other positive-strand RNA viruses such as Zika virus and Coxsackievirus B3.We speculate that Cvb D could be a broad-spectrum antiviral drug candidate for use against positive-strand RNA viruses that require autophagy for optimal replication.
