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用棘尾虫衰老无性系进行的灵芝抗细胞衰老研究 被引量:4
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作者 史新柏 《哈尔滨师范大学自然科学学报》 CAS 1998年第2期73-79,共7页
用两种灵芝制剂和一种灵芝生药的浸出液培养棘尾虫衰老的无性系细胞,直至它们死亡为止。和对照组的它们的姊妹细胞对比,发现实验组的细胞比对照组的存活时间更久,分裂次数更多。根据纤毛原生动物无性系的寿命的定义,灵芝的内含物延... 用两种灵芝制剂和一种灵芝生药的浸出液培养棘尾虫衰老的无性系细胞,直至它们死亡为止。和对照组的它们的姊妹细胞对比,发现实验组的细胞比对照组的存活时间更久,分裂次数更多。根据纤毛原生动物无性系的寿命的定义,灵芝的内含物延长了棘尾虫无性系细胞的寿命。灵芝抵抗单细胞动物衰老的效能提示作者应该进一步试验灵芝对多细胞动物和人体的效果。 展开更多
关键词 棘尾虫 灵芝 抗细胞衰老 衰老 无性系
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中医药延缓细胞衰老研究进展 被引量:2
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作者 李洋 胡光凤 +4 位作者 陈可 查江沙 钱来旺 张慧 渠景连 《亚太传统医药》 2023年第5期251-255,共5页
当代社会,老年人口比例呈现不断上升的趋势,老龄化问题日益严峻,因此,如何有效延缓衰老不仅成为了世界医学研究的热点,也成为了全球亟待解决的问题。中医药在延缓衰老领域有着独特的理论和丰富的实践经验,探讨传统医学理论及方药结合现... 当代社会,老年人口比例呈现不断上升的趋势,老龄化问题日益严峻,因此,如何有效延缓衰老不仅成为了世界医学研究的热点,也成为了全球亟待解决的问题。中医药在延缓衰老领域有着独特的理论和丰富的实践经验,探讨传统医学理论及方药结合现代分子机制延缓细胞衰老的研究进展,有助于对衰老过程的理解,防治衰老相关疾病。通过整理近年来国内外中医药抗细胞衰老作用机制的若干文献,对补气、补脾、滋阴、补肾、活血类中药通过减轻氧化应激损伤及调节免疫功能以延缓细胞衰老的机制进行分析、归纳与总结,以期为该领域未来深层次的研究提供新的见解。 展开更多
关键词 中医药 抗细胞衰老 氧化应激损伤 免疫功能 延缓衰老 研究进展
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干细胞原位培植四维抗衰老美容整形的临床应用研究(一)——不同年龄组面部软组织厚度变化 被引量:4
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作者 陈忠存 杨加峰 +3 位作者 顾永进 常晓静 陈惠惠 陈倩倩 《中国烧伤创疡杂志》 2002年第3期137-141,共5页
目的 :分析研究面部软组织厚度随年龄变化的关系。方法 :选择经过体脂含量计算正常者 14 0人 ,按不同年龄段分布测量发际、眉间、鼻根、鼻背、上唇根部、人中、颏唇沟、颏隆凸、颏下、眉中央、眶下缘中点、下颌下缘 (咬肌前 )、颧弓上... 目的 :分析研究面部软组织厚度随年龄变化的关系。方法 :选择经过体脂含量计算正常者 14 0人 ,按不同年龄段分布测量发际、眉间、鼻根、鼻背、上唇根部、人中、颏唇沟、颏隆凸、颏下、眉中央、眶下缘中点、下颌下缘 (咬肌前 )、颧弓上缘、下颌升支 (咬肌中 )、下颌角共 15个点 ,并记录制备图表与曲线。结果 :体脂正常者不同年龄组面部软组织厚度不同 ,而且面部软组织不同部位的厚度随年龄变化的程度也不同。结论 :衰老在面部的表现不仅仅是组织的松垂及皮肤皱纹的增多 。 展开更多
关键词 细胞原位培植四维衰老美容整形 临床应用 年龄 软组织厚度 面部 皮肤衰老整形
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我国干细胞抗衰老行业市场探析
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作者 王培 王岩 杨美家 《社会科学前沿》 2017年第7期848-854,共7页
随着生物技术日新月异的发展,干细胞抗衰老行业市场新产品新服务层出不穷并表现出强大的发展潜力。本文将通过数据分析及文献查询,从目前我国“干细胞抗衰老”技术的发展现状,来分析探讨其未来的市场发展前景,并对干细胞抗衰老行业提出... 随着生物技术日新月异的发展,干细胞抗衰老行业市场新产品新服务层出不穷并表现出强大的发展潜力。本文将通过数据分析及文献查询,从目前我国“干细胞抗衰老”技术的发展现状,来分析探讨其未来的市场发展前景,并对干细胞抗衰老行业提出相应的营销策略建议。 展开更多
关键词 细胞衰老 整形美容 经济前景 营销策略
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中药黄芪抗衰老研究展望
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作者 吴修一 《活力》 2004年第7期212-212,共1页
尽管目前有关衰老机理的学说甚多,但人们普遍认为遗传因素在生物衰老过程中起主导作用,即衰老的原发变化是发生在基因组水平上,因为转录及其调控是基因表达的一个重要方面,因而研究衰老过程中转录及其调控有何变化,对于探索衰老的... 尽管目前有关衰老机理的学说甚多,但人们普遍认为遗传因素在生物衰老过程中起主导作用,即衰老的原发变化是发生在基因组水平上,因为转录及其调控是基因表达的一个重要方面,因而研究衰老过程中转录及其调控有何变化,对于探索衰老的机制具有重要意义,对于抗衰老研究亦然如此。 展开更多
关键词 黄芪 衰老中药 机理研究 衰老细胞 分子药理学 二倍体细胞 体外培养寿命 抗细胞衰老效应
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椎间盘退行性变的机制及治疗方案研究进展
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作者 靳级 孙红 +2 位作者 庄勇 宁旭 刘淼 《实用医学杂志》 CAS 北大核心 2024年第22期3268-3274,共7页
椎间盘退行性变(intervertebral disc degeneration,IDD)是多种慢性脊椎疾病的主要致病因素,其主要临床症状包括下腰痛和急性下肢神经根性疼痛。近年来,随着人口老龄化进展加速及生活方式改变,IDD的发病率显著上升且呈现年轻化趋势,严... 椎间盘退行性变(intervertebral disc degeneration,IDD)是多种慢性脊椎疾病的主要致病因素,其主要临床症状包括下腰痛和急性下肢神经根性疼痛。近年来,随着人口老龄化进展加速及生活方式改变,IDD的发病率显著上升且呈现年轻化趋势,严重影响了患者的生活质量,并给社会带来了巨大的经济和医疗负担。如何有效防治IDD已成为当前亟待解决的医学难题。现有研究表明,椎间盘细胞衰老在IDD的发生与进展中扮演着重要角色,靶向衰老椎间盘细胞的治疗策略,如抗细胞衰老药物、基因编辑及干细胞疗法等,正逐渐成为研究热点。该文综述了椎间盘细胞衰老的分子机制,阐述了针对衰老细胞的多种治疗策略及最新进展,旨在为IDD的防治提供新的研究思路和治疗靶点,并为后续研究提供参考。 展开更多
关键词 椎间盘退行性变 椎间盘细胞衰老 细胞衰老通路 衰老细胞药物
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基于UPLC-triple-TOF-MS/MS比较桑树桑黄和瓦尼桑黄发酵产物的抗氧化活性 被引量:3
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作者 吕国英 宋婷婷 +2 位作者 张洋洋 蔡为明 张作法 《菌物学报》 CAS CSCD 北大核心 2023年第4期939-948,共10页
桑黄Sanghuangporus spp.是一类具有广泛的药理活性的大型真菌,其卓越的抗氧化活性备受关注。本研究比较了桑树桑黄Sanghuangporus sanghuang(桑黄)和瓦尼桑黄S.vaninii(杨黄)发酵产物乙醇提取物的抗氧化活性,并分析它们化学成分的差异... 桑黄Sanghuangporus spp.是一类具有广泛的药理活性的大型真菌,其卓越的抗氧化活性备受关注。本研究比较了桑树桑黄Sanghuangporus sanghuang(桑黄)和瓦尼桑黄S.vaninii(杨黄)发酵产物乙醇提取物的抗氧化活性,并分析它们化学成分的差异。实验以1,1-二苯基-2-三硝基苯肼(DPPH)清除率、羟基自由基引起的DNA损伤的保护作用和L929细胞抗衰老实验作为指标比较其抗氧化活性差异。结果表明,桑树桑黄和瓦尼桑黄发酵产物提取物均表现出良好的抗氧化性,而且桑树桑黄发酵物的抗氧化能力显著高于瓦尼桑黄。通过超高效液相色谱串联三重四级杆飞行时间质谱法(UPLC-triple-TOF-MS)从桑黄中共鉴定出11种多酚类物质,瓦尼桑黄发酵产物成分相对简单。 展开更多
关键词 桑黄 发酵产物 DNA保护 细胞衰老
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瓦尼桑黄多酚类化合物纯化及抗氧化活性 被引量:5
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作者 张洋洋 张作法 +2 位作者 宋婷婷 蔡为明 吕国英 《菌物学报》 CAS CSCD 北大核心 2023年第4期973-983,共11页
瓦尼桑黄中含有较多的酚类化合物,多酚类化合物具有较高的抗氧化活性。本研究采用深共熔溶剂(deep eutectic solvent,DES)提取多酚类化合物,通过单因素试验确定大孔树脂纯化桑黄多酚的最佳工艺参数。实验结果表明:HPD-100大孔树脂纯化... 瓦尼桑黄中含有较多的酚类化合物,多酚类化合物具有较高的抗氧化活性。本研究采用深共熔溶剂(deep eutectic solvent,DES)提取多酚类化合物,通过单因素试验确定大孔树脂纯化桑黄多酚的最佳工艺参数。实验结果表明:HPD-100大孔树脂纯化桑黄多酚的效果最好,其静态吸附-解吸最佳参数为:吸附时间为4 h,上样浓度10 mg/mL、上样液pH 4.0、解吸乙醇浓度为70%;动态吸附-解吸最佳参数为:上样量100mL、洗脱量110mL。在最佳条件下桑黄子实体多酚的纯度从14.56%提高到33.81%,纯化后的多酚具有良好的抗氧化活性能力。DPPH和ABTS自由基清除能力分别为92.75%和93.03%,对牛血清蛋白氧化损伤保护效果良好,能有效抑制L929细胞的衰老。 展开更多
关键词 瓦尼桑黄 大孔树脂 多酚 氧化活性 蛋白氧化损伤 细胞衰老
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Related gene expressions in anti-keratinocyte aging induced by Ganoderma lucidum polysaccharides 被引量:7
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作者 Xie Shaoqiong Liao Wanqing +1 位作者 Yao Zhirong Wang Zhidong 《Journal of Medical Colleges of PLA(China)》 CAS 2008年第3期167-175,共9页
Objective:To examine the level of expression of anti skin aging gene of Ganoderma lucidum polysacchayides and clarify its mechanism with anti aging of this ancient Chinese medicine.Methods:HacaT cell of keratinocytes ... Objective:To examine the level of expression of anti skin aging gene of Ganoderma lucidum polysacchayides and clarify its mechanism with anti aging of this ancient Chinese medicine.Methods:HacaT cell of keratinocytes lines were cultured and treated with the polysaccharides.The total RNA was extracted with Trizol reagent and cDNA was synthesized by reverse thanscription.The obtained cDNAs were then fluorescently labeled with cy3 and cy5 respectively and hybridized with gene expressing pedigree cDNA chip.The images were scanned and analyzed with special software.The scan data were analyzed with software and checked by real time PCR.Results:Among total 18 346 human genes,the expression of 103 ones was up-regulated and 378 ones down-regulated.It was demonstrated evidently that Ganoderma lucidum polysaccharides affected the expression of genes of anti skin aging.Two ways are anastomotic.Conclusion:it is concluded by analysis of function of these up-regulation and down-regulation genes that Ganoderma lucidum polysaccharides may play an important role in boosting cell growth and against skin aging.It shows that the results of gene array reliable by real time PCR. 展开更多
关键词 Ganoderma lucidum polysaccharides Skin aging Gene chip KERATINOCYTE
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Targeting of AUF1 to vascular endothelial cells as a novel anti-aging therapy
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作者 Jian HE Ya-Feng JIANG +7 位作者 Liu LIANG Du-jin WANG Wen-Xin WEI Pan-Pan JI Yao-Chan HUANG Hui SONG Xiao-Ling LU Yong-Xiang ZHAO 《Journal of Geriatric Cardiology》 SCIE CAS CSCD 2017年第8期515-523,共9页
Background Inhibition of aging of vascular endothelial cells (VECs) may delay aging and prolong life. The goal of this study was to prepare anti-CD31 monoclonal antibody conjugated PEG-modified liposomes containing ... Background Inhibition of aging of vascular endothelial cells (VECs) may delay aging and prolong life. The goal of this study was to prepare anti-CD31 monoclonal antibody conjugated PEG-modified liposomes containing the AU-rich region connecting factor 1 (AUF1) gene (CD31-PILs-AUF1) and to explore the effects of targeting CD31-PILs-AUF1 to aging VECs. Methods The mean particle sizes of various PEGylated immunoliposomes (PILs) were measured using a Zetasizer Nano ZS. Gel retardation assay was used to confirm whether PILs had encapsulated the AUF1 plasmid successfully. Fluorescence microscopy and flow cytometry were used to quantify binding of CD31-PILs-AUF1 to target cells. Flow cytometry was also used to analyze the cell cycles of aging bEnd3 cells treated with CD31-PILs-AUF1. We also developed an aging mouse model by treating mice with D-galactose. Enzyme-linked immunosorbent assay (ELISA) was used to evaluate the levels of interleuldn-6 (IL-6) and tumor necrosis factor-or (TNF-ct). The malondialdehyde (MDA) and the superoxide dismutase (SOD) levels were detected by commercial kits. Hematoxylin-eosin (HE) staining was used to determine whether treatment with CD31-PILs-AUF 1 was toxic to the mice. Results CD31-PILs-AUF 1 specifically could targeted bEnd3 VECs and increased the percentage of cells in the S and G2/M phases of aging bEnd3 cells. ELISA showed that content of the IL-6 and TNF-ct decreased in CD31-PILs-AUF1 group. The level of SOD increased, whereas MDA decreased in the CD31-PILs-AUF1 group. Additionally, CD31-PILs-AUF 1 was not toxic to the mice. Conclusion CD31-PILs-AUF 1 targets VECs and may delay their senescence. 展开更多
关键词 Aging AU-rich region connecting factor 1 PEGylated immunoliposomes Vascular endothelial ceils
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精液有延长寿命的功效
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《生活与健康》 2010年第7期51-51,共1页
奥地利格拉茨大学的研究人员认为,人类精液中有种化合物——亚精胺,可以减少体内自由基,具有抗细胞衰老的作用,对延长寿命有神奇的功效。格拉茨大学的生化学家弗兰克·马德欧和托拜·厄斯森伯格共同领导一个团队,该团队的... 奥地利格拉茨大学的研究人员认为,人类精液中有种化合物——亚精胺,可以减少体内自由基,具有抗细胞衰老的作用,对延长寿命有神奇的功效。格拉茨大学的生化学家弗兰克·马德欧和托拜·厄斯森伯格共同领导一个团队,该团队的宗旨是来测试一种叫做亚精胺物质的影响。 展开更多
关键词 人类精液 功效 寿命 抗细胞衰老 研究人员 亚精胺 奥地利 化合物
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Anti-senescence effect and molecular mechanism of the major royal jelly proteins on human embryonic lung fibroblast(HFL-I) cell line 被引量:6
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作者 Chen-min JIANG Xin LIU +4 位作者 Chun-xue LI Hao-cheng QIAN Di CHEN Chao-qiang LAI Li-rong SHEN 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2018年第12期960-972,共13页
Royal jelly (R J)from honeybee has been widely used as a health promotion supplement.The major royal jelly proteins (MRJPs)have been identified as the functional component of RJ.However,the question of whether MRJPs h... Royal jelly (R J)from honeybee has been widely used as a health promotion supplement.The major royal jelly proteins (MRJPs)have been identified as the functional component of RJ.However,the question of whether MRJPs have anti-senescence activity for human cells remains.Human embryonic lung fibroblast (HFL-I)cells were cultured in media containing no MRJPs (A),MRJPs at 0.1mg/ml (B),0.2mg/ml (C),or 0.3mg/ml (D),or bovine serum albumin (BSA)at 0.2mg/ml (E).The mean population doubling levels of cells in media B,C,D,and E were increased by 12.4%,31.2%,24.0%,and 10.4%,respectively,compared with that in medium A.The cells in medium C also exhibited the highest relative proliferation activity,the lowest senescence,and the longest telomeres.Moreover, MRJPs up-regulated the expression of superoxide dismutase-1(SOD1)and down-regulated the expression of mammalian target of rapamycin (MTOR),catenin beta like-1(CTNNB1),and tumor protein p53(TP53).Raman spectra analysis showed that there were two unique bands related to DNA synthesis materials,amide carbonyl group vibrations and aromatic hydrogens.These results suggest that MRJPs possess anti-senescence activity for the HFL-I cell line,and provide new knowledge illustrating the molecular mechanism of MRJPs as anti-senescence factors. 展开更多
关键词 Major royal jelly protein Human embryonic lung flbroblast (HFL-I)cell line Anti-senescence Relative proliferation activity Telomere length Molecular mechanism
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Effects of mild-warming moxibustion on Bcl-2 and PKC expressions of peripheral blood in elderly people 被引量:3
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作者 施茵 崔云华 +7 位作者 吴焕淦 张卫 赵琛 刘慧荣 国兰琴 吴蓓玲 余爱珍 张英英 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2012年第1期45-51,共7页
OBJECTIVE:To explore the anti-aging effects of mild-warming moxibustion on Bcl-2 and PKC expression in peripheral blood and general symptoms in elderly people.METHODS:A total of 61 elderly people and 30 non-elderly pe... OBJECTIVE:To explore the anti-aging effects of mild-warming moxibustion on Bcl-2 and PKC expression in peripheral blood and general symptoms in elderly people.METHODS:A total of 61 elderly people and 30 non-elderly people were enrolled.The total effective rate of mild-warming moxibustion was assessed by symptom scores,and Bcl-2 and PKC expression in peripheral blood was detected by flow cytometry.RESULTS:The total effective rate in the mild-warming moxibustion group was significantly higher than in the blank control group(P<0.01).Bcl-2 and PKC expression rates in peripheral blood in the blank control group were lower than in the normal control group(P<0.01),but higher after mild-warming moxibustion(P<0.01).CONCLUSION:The anti-aging effects of mild-warm-ing moxibustion may be due to increased Bcl-2 and PKC expression in peripheral blood in aged people. 展开更多
关键词 Mild-warming moxibustion Elderly BCL-2 PKC Clinical study
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Porcine placental peptides improve neuroblast proliferation and differentiation via enhancement of TrkB and BDNF levels in D-glatacose-induced mouse aging model
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作者 Xu Quan Caihong Gu +5 位作者 Feng Yan Yaoyao Gao Lin Chen Rong Wei Bingchun Yan Rong Hul 《Journal of Chinese Pharmaceutical Sciences》 CAS CSCD 2019年第10期739-748,共10页
Aging affects the nervous system as well as other organs.In our study,we aimed to study the pharmacological effects and mechanism of porcine placental peptides(PPP)in aging process,and to observe the changes of neurob... Aging affects the nervous system as well as other organs.In our study,we aimed to study the pharmacological effects and mechanism of porcine placental peptides(PPP)in aging process,and to observe the changes of neuroblast proliferation and differentiation as well as partial gene expression in hippocampus of D-galactose-induced aged mouse.Based on the analysis of experimental results,it was confirmed that PPP significantly improved neurobalst proliferation and differentiation in the mouse hippocampal DG by ki-67 and DCX immunohistochemistry.This result showed that PPP had anti-aging effects on D-galactoseinduced aging mouse model.Moreover,we observed up-regulated expressions of BDNF and TrkB proteins and down-regulated expressions of Caspase 3,8,and 9 proteins in the PPP-treated mouse hippocampus.Therefore,our results showed that PPP obviously improved neuroblast proliferation and differentiation,and its anti-aging effect might berelated to down-regulation of apoptosis-related proteins,including Caspase 3,8,and 9,via BDNF/TrkB pathway.Our findings provided valuable evidence for its applications in the health and medicine sectors. 展开更多
关键词 Porcine placenta peptide ANTI-AGING Neuroblast proliferation and differentiation Apoptosis-related proteins BDNF/TrkB pathway
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