期刊文献+
共找到2篇文章
< 1 >
每页显示 20 50 100
抗胃癌细胞系GC 79-11单克隆抗体的研制及鉴定
1
作者 孙亚萍 周红 +3 位作者 吴晰莹 颜江华 杨善民 陈美玉 《单克隆抗体通讯》 CSCD 1993年第4期51-52,共2页
近十年来,已相继研制成功多株胃癌单克隆抗体,但有关胃癌单克隆抗体在临床上的应用价值仍在探索中。因此,选择不同类型的胃癌细胞作为免疫原,制备针对不同的胃癌相关抗原的单克隆抗体,对于胃癌的诊断和治疗具有重要的意义。
关键词 抗胃癌细胞系 GC79-11细胞 研制
下载PDF
Activation of STAT3 signaling in human stomach adenocarcinoma drug-resistant cell line and its relationship with expression of vascular endothelial growth factor 被引量:20
2
作者 Li-FenYu YingCheng Min-MinQiao Yong-PingZhang Yun-LinWu 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第6期875-879,共5页
AIM: To investigate the difference in activation of STAT3 signaling between two human stomach adenocarcinoma cell lines: 5-fluorouracil resistant cell line and its parental cell line, and to evaluate its relationship ... AIM: To investigate the difference in activation of STAT3 signaling between two human stomach adenocarcinoma cell lines: 5-fluorouracil resistant cell line and its parental cell line, and to evaluate its relationship with the expression of vascular endothelial growth factor (VEGF). METHODS: Western blot and electrophoretic mobility shift assay (EMSA) were used to detect the expression of phospho-STAT3 protein and constitutive activation of STAT3 in two human stomach adenocarcinoma cell lines, 5-fluorouracil resistant cell line SGC7901/R and its parental cell line SGC7901, respectively. The mRNA expression of VEGF was analysed by semi-quantitative RT-PCR. The expressive intensity of VEGF protein was measured by immunocytochemistry. RESULTS: The expressions of phospho-STATS protein and constitutive activation of STAT3 between two human stomach adenocarcinoma cell lines were different. Compared with the parental cell line SGC7901, the STAT3DNA binding activity and the expressive intensity of phospho-STAT3 protein were lower in the drug-resistant cell line SGC7901/R. The expression levels of VEGF mRNA and its encoded protein were also decreased in drugresistant cell line. CONCLUSION: Over-expression of VEGF may be correlated with elevated STAT3 activation in parental cell line. Lower VEGF expression may be correlated with decreased STAT3 activation in resistant cell line, which may have resulted from negative feedback regulation of STAT signaling. 展开更多
关键词 Stomach adenocarcinoma Vascular endothelial growth factor STAT3 protein Antineoplastic Drug Resistance
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部