在目前执行的糖尿病分型,是依据ADA的建议,于1997年提出的糖尿病的病因学分类中,其中一项即胰腺外分泌疾病致糖尿病,包括胰腺肿瘤;在1985年版的分类中的胰源性糖尿病;明确提出胰腺癌,近年来关于胰腺癌和糖尿病的关系可能已经跳出胰源性...在目前执行的糖尿病分型,是依据ADA的建议,于1997年提出的糖尿病的病因学分类中,其中一项即胰腺外分泌疾病致糖尿病,包括胰腺肿瘤;在1985年版的分类中的胰源性糖尿病;明确提出胰腺癌,近年来关于胰腺癌和糖尿病的关系可能已经跳出胰源性糖尿病的概念。1胰腺癌（carcinoma of the pmcrease）主要指胰腺外分泌腺癌。约占消化道恶性肿瘤的10%,第4位,近年来发病率在明显上升,恶性程度高,是男性肿瘤患者死亡的第七大死因。展开更多
To get recombinant antigen (Is/et Cell Autoantigen 69)ICA69 which was expressed in Escherichia coli strains (E.coli) by means of the gene engineering technique so that it can be used for early diagnosis of and screeni...To get recombinant antigen (Is/et Cell Autoantigen 69)ICA69 which was expressed in Escherichia coli strains (E.coli) by means of the gene engineering technique so that it can be used for early diagnosis of and screening in type Ⅰ diabetes mellitus, the cDNA fragment of human ICA69 was amplified by PCR, and then cloned into pSPORT 1 vector. After DNA sequencing, it was inserted into pGEX-2T between the sites of EcoR Ⅰ and Sma Ⅰ, then recombinant plasmid p2T-ICA69 was constructed and introduced into E.coli. The GST-ICA69 fusion protein was expressed by the induction of IPTG. The recombinant ICA69 proteins were used to detect the antibodies against hICA69 in 100 healthy subjects and type Ⅰ diabetic serum by the use of indirect ELISA. The sequence analysis showed that the amplified fragments contained 1449 bp, encoded 483 amino acids, and had been correctly inserted into pGEX-2T vector. The recombinant proteins expressed in the prokaryotic cells had immunogenicity and could be used to detect antibodies against ICA69 in type Ⅰ diabetic serum. Finally it can be concluded in this paper that the expression products obtained by the method of gene engineering are recombinant ICA69 antigen and may be used to improve the forecast rate and the diagnostic rate of type Ⅰ diabetes in combination with other tests.展开更多
文摘在目前执行的糖尿病分型,是依据ADA的建议,于1997年提出的糖尿病的病因学分类中,其中一项即胰腺外分泌疾病致糖尿病,包括胰腺肿瘤;在1985年版的分类中的胰源性糖尿病;明确提出胰腺癌,近年来关于胰腺癌和糖尿病的关系可能已经跳出胰源性糖尿病的概念。1胰腺癌（carcinoma of the pmcrease）主要指胰腺外分泌腺癌。约占消化道恶性肿瘤的10%,第4位,近年来发病率在明显上升,恶性程度高,是男性肿瘤患者死亡的第七大死因。
文摘To get recombinant antigen (Is/et Cell Autoantigen 69)ICA69 which was expressed in Escherichia coli strains (E.coli) by means of the gene engineering technique so that it can be used for early diagnosis of and screening in type Ⅰ diabetes mellitus, the cDNA fragment of human ICA69 was amplified by PCR, and then cloned into pSPORT 1 vector. After DNA sequencing, it was inserted into pGEX-2T between the sites of EcoR Ⅰ and Sma Ⅰ, then recombinant plasmid p2T-ICA69 was constructed and introduced into E.coli. The GST-ICA69 fusion protein was expressed by the induction of IPTG. The recombinant ICA69 proteins were used to detect the antibodies against hICA69 in 100 healthy subjects and type Ⅰ diabetic serum by the use of indirect ELISA. The sequence analysis showed that the amplified fragments contained 1449 bp, encoded 483 amino acids, and had been correctly inserted into pGEX-2T vector. The recombinant proteins expressed in the prokaryotic cells had immunogenicity and could be used to detect antibodies against ICA69 in type Ⅰ diabetic serum. Finally it can be concluded in this paper that the expression products obtained by the method of gene engineering are recombinant ICA69 antigen and may be used to improve the forecast rate and the diagnostic rate of type Ⅰ diabetes in combination with other tests.
